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Citations for GE Life Sciences :
451 - 500 of 661 citations for Yellow Fever virus lysate 17D strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... The proteins from the cleared cell lysate were captured in a HisTrap HP 5 ml column (GE Healthcare) in His buffers (20 mM MOPS ...
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bioRxiv - Biochemistry 2020Quote: ... and the crude lysates were applied to 5 ml HiTrap Capto Q column (GE Healthcare Life Sciences, USA) equilibrated with 5 volumes of buffer A (20 mM Tris (pH 8.0) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell lysate was clarified by centrifugation and loaded onto a HisTrap FF column (GE Healthcare, Piscataway, NJ) that had been pre-equilibrated with 10 column volumes of binding buffer (50 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2021Quote: ... The cleared lysate was filtered through a 0.45µm filter and loaded on a HisTrap HP column (GE Healthcare). The full length Clr4 was enriched from the cleared lysate by ammonium sulfate fractionation with 40% NH4SO4 ...
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bioRxiv - Immunology 2020Quote: Cell lysates (10μg protein) were resolved by electrophoresis and transferred to a polyvinylidene fluoride membrane (GE Healthcare, UK). Membranes were blocked for 1 hour in 5% skimmed milk/TBS/0.1% Tween-20 and incubated with primary antibodies (1:1000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... HemO lysate was applied to a Q-Sepharose Fast Flow column (2.5 cm × 6 cm) (GE Life Sciences) equilibrated with 20 mM Tris (pH 8.0 at 4°C) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The lysate obtained was loaded onto the pre-equilibrated nickel affinity column (HisTrap FF, 5 mL, GE Healthcare) with loading buffer (50 mM KPi ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were run on SDS-polyacrylamide gel and transferred to nitrocellulose membranes Protran BA 85 (GE Healthcare). The membranes were incubated with the antibodies indicated above and visualized with horseradish peroxidase–conjugated secondary antibodies using the ECL Western blotting system (Thermo Scientific).
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bioRxiv - Physiology 2019Quote: ... The vector was purified from the clarified lysate by affinity chromatography over a HiTrap heparin column (GE Healthcare), and ultracentrifuged overnight before resuspension in sterile physiological Ringer’s solution ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared lysate was filtered (0.8 µM) and applied to 5 or 10 ml HisTrap FF (GE Healthcare) equilibrated in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Systems Biology 2019Quote: ... Harvested cells were sonicated for lysis and clarified lysate was loaded onto a HisTrap FF column (GE Healthcare). The His tag was cleaved by overnight incubation at 4 °C with SUMO protease (Invitrogen) ...
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bioRxiv - Synthetic Biology 2019Quote: The clean lysate was next loaded onto a HisTrap HP column (HisTrap FF crude 1 ml, GE Healthcare) at 4°C and flow rate of 0.5 ml min−1 ...
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bioRxiv - Genetics 2019Quote: The clarified lysate containing mZCWPW1 was then loaded onto a 5 mL His-Trap HP column (GE Healthcare) using NGCTM chromatography system (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... The clarified lysate was first affinity-purified on an ÄKTA Purifier using His-Trap HP columns (GE Healthcare) and eluted with elution buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
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bioRxiv - Cell Biology 2022Quote: ... Clear lysate was incubated with 0.5 ml packed beads IgG Sepharose 6 Fast-Flow (GE Healthcare, Chicago, IL) for 2-3 hours at 4°C on a tube roller ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were incubated with mouse monoclonal anti-MYC-antibody (Covance) and protein G-agarose beads (GE Healthcare). The precipitates were then washed with a lysis buffer ...
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bioRxiv - Physiology 2023Quote: ... The vector was purified from the clarified lysate by affinity chromatography using a HiTrap heparin column (GE Healthcare), ultracentrifuged overnight and resuspended in sterile Ringer’s solution ...
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bioRxiv - Bioengineering 2023Quote: ... The ErCas12a protein in clarified lysate was sequentially purified using immobilized metal affinity chromatography (HisTrap HP, GE Healthcare) and heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Microbiology 2023Quote: ... The cell-free lysate was applied to a 5 ml His Trap™ HP column (GE Healthcare, USA) equilibrated in the same buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered lysate was applied to a 1-ml His-Trap (all FPLC columns were obtained from GE Healthcare) column using an ÄKTA FPLC ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was cleared by centrifugation and loaded on a Ni-NTA agarose column (HisTrap HP; GE Healthcare). After rigorous washing with 100 mM Tris-HCl pH 8.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Lysate samples were separated by SDS-PAGE or LDS-NuPAGE and transferred to 0.2μm nitrocellulose membrane (GE Healthcare) via tank immersion ...
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bioRxiv - Neuroscience 2020Quote: ... This was expressed in E .coli strain NiCo21 (DE3) using conditions previously described(96) and purified by IMAC using NiHP (GE Healthcare Life Sciences) and AEC using QHP (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2019Quote: Recombinant GST and GST-HIT proteins were produced by IPTG induction of transformed BL21 Rosetta strains and purified on 100 μl of Glutathione Sepharose 4B beads (17075601, GE Healthcare Life Sciences), for 4-5 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was incubated with Glutathione Sepharose beads (20 μL for one group, 17-0756-01, GE healthcare, Sweden) at 4 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... the clarified lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare). Proteins were step-eluted with elution buffer (50 mM Tris-HCl pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... XPG and XPA containing lysate was applied onto GE HisTrap HP 5 mL (GE Healthcare, Little Chalfont, United Kingdom) equilibrated in lysis buffer (in case of XPA all downstream steps were performed in the presence of 5 mM β-mercaptoethanol and 10 μM ZnCl2 instead of 1 mM DTT) ...
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bioRxiv - Molecular Biology 2019Quote: ... Lysate was pre-incubated with 200 μL of rehydrated Protein A Sepharose CL-4B beads (GE Healthcare, Life Sciences) for 15 minutes at 4°C with rocking ...
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bioRxiv - Biophysics 2019Quote: ... Lysates were cleared by centrifugation (10000 rpm) and incubated with Glutathione Sepharose 4B beads (GE Life Sciences, Freiburg, Germany) on a rotating platform at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were centrifuged and the supernatants used for the purification on HisTrap HP columns (1 mL; GE Healthcare) connected to an AKTA Explorer system (GE Healthcare) ...
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bioRxiv - Neuroscience 2021Quote: ... The lysate was centrifuged and the clarified supernatant loaded onto a 5ml HisTrap HP nickel affinity column (GE Healthcare). dCry protein was eluted with 100 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Plant Biology 2020Quote: ... the clarified lysate was applied to a Ni2+-NTA column connected to an AKTA Xpress purification system (GE Healthcare). Proteins were step-eluted with elution buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was clarified by centrifugation at 40,000 rpm for 45 minutes and loaded onto a HisTrap column (GE Healthcare) equilibrated in nickel buffer A (50 mM HEPES (pH 7.6) ...
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bioRxiv - Cancer Biology 2021Quote: ... The soluble fraction of the cell lysate was purified on HiTrap IMAC HP columns (GE Healthcare # 17-0920-05) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were diluted with PBS to 10 ml and incubated with 200 μl of GST-Sepharose (GE Healthcare) for 1 hour on ice under agitation ...
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bioRxiv - Microbiology 2021Quote: ... and the filtered lysate was passed sequentially through HisTrap Excel nickel and HiTrap CP cation exchange columns (GE Healthcare) to purify the tagged TOP2α protein before incubation overnight with His-tagged TEV protease ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified cell lysate produced as for D2-I was loaded onto a HisTrap HP column (GE Healthcare Life Sciences). Using an imidazole gradient ...
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bioRxiv - Biophysics 2019Quote: ... Cell lysates containing Hb were initially purified using a Zn2+ charged chelating Sepharose Fast Flow resin column (GE healthcare) because Hb surfaces are rich with His residues that can bind Zn2+ (also see (42)) ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate is incubated with 100ul of equilibrated IgG slurry (IgG SepharoseTM 6 Fast Flow from GE Healthcare) overnight in rotator wheel at 4°C with slow rotation ...
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bioRxiv - Biophysics 2020Quote: ... The protein was purified from the supernatant of the lysate using a 5 ml HisTrap HP column (GE Healthcare). The Smt3 tag was cleaved off before further purification by a HiLoad 16/600 Superdex 75 size exclusion column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... In order to purify MIF the lysate was first passed over a 5-mL CM-sepharose column (GE Healthcare), and after washing with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by centrifugation and SUMO-tagged IMPDH chromatographically purified with HisTrap FF columns (GE Healthcare Life Sciences) and an Äkta Start chromatography system ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was filtered (0.45 µm) and then loaded onto a 5 ml HisTrap FF Crude column (GE Healthcare) equilibrated with wash buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were clarified by centrifugation and incubated with 20 mM imidazole on Ni Sepharose High Performance Resin (GE Healthcare) at 4°C for 4 hours ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was pelleted and cell lysate was filtered with a 0.2μm filter and loaded onto a HisTrap Ni column (GE Healthcare) at 4°C ...