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Citations for GE Life Sciences :
351 - 400 of 4289 citations for Yellow Fever Virus Envelope Protein Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Protein G sepharose beads (GE Healthcare) were washed three times in lysis buffer to remove any residual ethanol from the storage buffer ...
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bioRxiv - Cell Biology 2020Quote: ... and Protein G Sepharose (GE Healthcare) pre-blocked in 1% BSA-PBS were used to immunoprecipitate (IP ...
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bioRxiv - Cell Biology 2020Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Molecular Biology 2021Quote: ... A Protein-G column (GE Healthcare) was used for affinity purification of antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-G sepharose beads (GE Healthcare) were spun at 13000X g for 1 minute ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G-agarose beads (GE Healthcare) were added to the lysate–antibody mix and left to rock at 4°C for 2 h ...
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bioRxiv - Plant Biology 2020Quote: ... RDR2 and protein standards (GE Healthcare) were subjected to FPLC using a Superdex 200 10/300 GL column at 4°C using 50 mM HEPES-KOH pH7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... protein G Sepharose beads (GE Healthcare) were blocked overnight with 0.5 mg BSA ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare) was added to Expi293 supernatant for 2h at room temperature or overnight at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... Protein A sepharose beads (GE Healthcare) were washed twice and resuspended 1:1 with lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... and Protein G sepharose (GE Healthcare) respectively ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G-Sepharose beads (GE Healthcare) were added to the supernatant and incubated at 4°C for 3 h ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G–agarose beads (GE Healthcare) or magnetic beads were washed twice before immunoprecipitation.
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bioRxiv - Neuroscience 2023Quote: ... Protein-G Sepharose beads (GE Healthcare) were blocked in 0.1% BSA for 1 hour at room temperature ...
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bioRxiv - Genetics 2023Quote: ... and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... with protein A column (GE Healthcare). MARV GP (Uganda strain ...
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bioRxiv - Biochemistry 2023Quote: ... KTA Protein Purification Systems (GE Healthcare) and UNICORN Control Software version 5.11 (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins and SP3 beads (GE healthcare) were mixed in a 1:10 weight ratio in 80 % ethanol for 5 minutes at RT ...
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bioRxiv - Molecular Biology 2024Quote: Protein A-Sepharose beads (GE Healthcare) were incubated with 2 µg of antibody (anti-V5 ...
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Immunology 2022Quote: ... followed by horseradish peroxidase (HRP)-labeled secondary antibody goat-anti-mouse IgG Fc (Product #: 31433) (Pierce Antibodies) or donkey-anti-rabbit IgG (Product #: NA934V) (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Immunology 2019Quote: ... followed by horseradish peroxidase (HRP)-labelled secondary antibody goat-anti mouse IgG Fc (Pierce Antibodies) or donkey-anti-rabbit IgG (GE Life Sciences). HRP conjugates bound to antigen were detected and visualized by using an ECL detection kit (GE Life Sciences).
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bioRxiv - Molecular Biology 2019Quote: ... fused to triple epitope tags were prepared by directionally cloning in-frame epitope tags into the BamHI and EcoRI restriction sites of pGEX-4T-1 (GE Healthcare Life Sciences #28954549). PCR amplicons were prepared from a PAGE-purified oligonucleotide template with forward primers containing a BglII restriction site and reverse primers containing HindIII–BamHI–EcoRI sites (Supplementary Table S1) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified via a two-step chromatography process involving nickel-affinity chromatography via the encoded C-terminal hexa-histidine tag (HisTrap™ Ni2+-NTA column; GE Healthcare Life Sciences), and size-exclusion chromatography (Superdex S200 10/300 GL column ...
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bioRxiv - Molecular Biology 2020Quote: ... 4.5 µL of Cy5 Mono-Reactive NHS Ester Fluorescent Dye (ChIP samples) or Cy3 Mono-Reactive NHS Ester Fluorescent Dye (control samples Input DNA or no-tag ChIP) (GE Healthcare, PA25001 and PA23001) were added ...
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bioRxiv - Neuroscience 2023Quote: ... The remaining tag and protease were removed by reverse Ni-NTA purification in batch mode (Ni-Sepharose High Performance; GE Healthcare, 17-5268-02). Final polishing of the target proteins was done through size exclusion on a HiLoad 26/600 Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... was pre-incubated with 50 μl Protein A and Protein G Sepharose (GE Healthcare 17513801 and 17061801) mix for 3~4 h and then incubated with WCE overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The protein-protein complex was then passed through a size-exclusion column (Superdex75, 26/60, GE Healthcare) to remove unbound proteins ...
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bioRxiv - Microbiology 2021Quote: ... Secreted proteins were purified from the culture supernatants using a protein A+G affinity column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... except that antibody-bound protein complexes were isolated with protein A sepharose beads (GE Healthcare Life Sciences) washed with RIPA buffer (140mM NaCl ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
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bioRxiv - Microbiology 2020Quote: Binding kinetics and affinities for ACE2.Fc were assessed using surface plasmon resonance technology on a Biacore T200 instrument (GE Healthcare, Marlborough, MA) using a Series S CM5 sensor chip in filtered and degassed HBS-EP running buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... IgG proteins were expressed in transiently-transfected Expi293F cells and purified by protein A affinity chromatography (GE Healthcare) followed by SEC as described21,47.
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... Protein purity was estimated as >95% by SDS-PAGE and protein concentration was measured spectrophotometrically (NanoVue, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Biophysics 2020Quote: ... thrombin cleavage of the His6-tag overnight and final ion exchange chromatography with a MonoQ or MonoS column (Äkta purifying system, GE Healthcare, Little Chalfont, United Kingdom). Synaptobrevin 2 49-96 S79C was labeled with Atto488 maleimide ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatants were filtrated and subjected to affinity chromatography using a 5 mL HisTrap HP His-tag purification column (GE healthcare, cat. # GE29-0510-21) operated by a peristaltic pump (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... the removal of SUMO protease and SUMO tag from the cleavage reaction was performed with affinity chromatography on a HisTrap HP column (GE Healthcare, Code#:17-5248-01). The SUMO protease and SUMO tag will stay in the HisTrap HP column and the cleaved native protein will be in the flow-through fractions ...
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bioRxiv - Biochemistry 2022Quote: ... The cDNAs of full-length TPR-CHAT were synthesized using the bacterial expression vector pGEX-6P-1 (GE Healthcare, with an N-terminal GST tag). Point mutations were introduced using Fast Site-Directed Mutagenesis Kit in the Sb-gRAMP gene and verified by DNA sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... and Protein G Mag Sepharose (GE healthcare). The DIG-labeled 3’UTR of Emi2 and cyclin B1 RNA probes were synthesized with a DIG-RNA-labeling kit (Roche Molecular Biochemicals) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein A-agarose beads (GE Healthcare, USA) were incubated with the supernatant at 4 °C for 2 h ...
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bioRxiv - Molecular Biology 2019Quote: The beads (G protein Sepharose, GE Healthcare) were washed twice in IP buffer and centrifuged for 2 min at 2’700 x g before blocking with 1 mg/ml BSA in IP buffer for 1 h ...
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bioRxiv - Physiology 2021Quote: ... Protein G Sepharose was from GE Healthcare. Rhodocytin and collagen related peptide (CRP ...
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bioRxiv - Biochemistry 2022Quote: ... and protein A affinity chromatography (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... together with protein A Sepharose (GE Healthcare), respectively ...
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bioRxiv - Immunology 2022Quote: ... Protein A Sepharose (GE Healthcare cat.# 45002982) and Protein G Sepharose (GE Healthcare cat.# 45000118 ...