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Citations for GE Life Sciences :
1 - 50 of 675 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Pek1 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pGEX-4T-1 (GE Healthcare) or pMAL-c2x (New England Biolabs) ...
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bioRxiv - Cell Biology 2020Quote: Yeast Cka1 and Tda1 genes were amplified by PCR from yeast genomic DNA and then cloned into pGEX4T-1 (GE healthcare) vector ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant of yeast lysate was incubated with calmodulin resin (GE Healthcare) overnight at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant of yeast lysate was incubated with calmodulin resin (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: Expression vector pGEX-6P1 (GE Healthcare) –based recombinant synthesis of CPK21 and CPK23 constructs carrying a C-terminal polyhistidine-tag and N-terminal GST-tag has been described previously (Geiger et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The yeast knockout strain collection was originally purchased from GE Healthcare (cat. # YSC1053).
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bioRxiv - Cell Biology 2023Quote: ... The yeast knockout strain collection was originally purchased from GE Healthcare (cat. # YSC1053).
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bioRxiv - Cell Biology 2020Quote: ... The two fragments were fused by PCR to obtain a product corresponding to the 783 bp coding sequence of TMEM70 transcript variant 1 that was cloned into pMOSblue vector using the Blunt-Ended PCR Cloning Kit (GE Healthcare Life Sciences) to generate the pMOS-TMEM70 plasmid ...
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bioRxiv - Microbiology 2022Quote: ... using the pGEX4T1 expression vector (GE Healthcare). Protein expression was induced at 37°C for 3 h using 0.5 mM isopropyl-D-thiogalactopyranoside ...
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bioRxiv - Biochemistry 2020Quote: ... The yeast TET-promoters Hughes Collection strains were purchased from Dharmacon (GE Healthcare, Lafayette, CO). Tetracycline-inducible strains were transformed with GPD-DED1 ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 5 μg of PCR products were transformed into the haploid yeast strain BY4741 (GE Healthcare Dharmacon Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... The viability was quantified by staining the yeast cells in 0.1% Trypan blue staining (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cancer Biology 2019Quote: ... cloned into the expression vector pGEX-2T (GE Healthcare), were expressed in E ...
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bioRxiv - Biochemistry 2019Quote: Yeast were imaged at 30 °C in an Environmental Chamber with a DeltaVision Spectris (Applied Precision Ltd.) comprising a wide-field inverted epifluorescence microscope (IX70 ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast images were acquired using a Deltavision RT microscope system running softWoRx 3.5.1 software (Applied Precision, LLC), using an Olympus 100× ...
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bioRxiv - Molecular Biology 2023Quote: ... Yeast images were acquired using a Deltavision RT microscope system running softWoRx 3.5.1 software (Applied Precision, LLC), using an Olympus 100× ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μg Cas9 expression plasmid (GE Healthcare #U-005100-120), and 10 μg repair plasmid (Genscript ...
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bioRxiv - Microbiology 2023Quote: ... and cloning it into the EcoRI and SalI sites of pGEX-4T-1(GE Healthcare) in frame with glutathione S-transferase (GST ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Bioengineering 2020Quote: ... for IgG expression and 5 ml kappa-select column (GE Healthcare) for Fab purification ...
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bioRxiv - Molecular Biology 2019Quote: ... coli cells were transformed with pGEX6 bacterial expression constructs (GE Healthcare) harboring GST-SBP (streptavidin binding peptide)-RGG or the two KH domains from mouse FMRP ...
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bioRxiv - Microbiology 2020Quote: ... coli Rosetta 2 from pGEX-6P1 expression vector (GE Healthcare, USA). To improve the solubility of the full-length protein ...
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bioRxiv - Genomics 2023Quote: ... Yeast cells were harvested by filtration with 0.45 µM Whatman cellulose nitrate membrane filters (GE Life Sciences #7184-004). Ribosome profiling was conducted as detailed in (McGlincy and Ingolia ...
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bioRxiv - Cell Biology 2020Quote: All yeast live-cell epifluorescent microscopy was performed on a Nikon Eclipse E600 or a Delta Vision microscope (Applied Precision) equipped with a Coolsnap HQ camera (Photometrics) ...
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bioRxiv - Cell Biology 2022Quote: A dual expression vector was created on a pGEX2T backbone (GE Healthcare) and two plasmids were generated for co-expression of IFT25 and IFT27 or its mutants (IFT27S79L and IFT27S30N ...
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bioRxiv - Cell Biology 2022Quote: ... and was cloned in the expression vector pGEX-4T-1 (GE Healthcare). Recombinant CD47 was expressed as a fusion protein with glutathione S-transferase (GST ...
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bioRxiv - Cancer Biology 2022Quote: ... WT and mutant Ankrd26 N-Ank modules were generated by cloning into pGEX-6P-1 (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... generated by cloning of corresponding ORFs between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare), expressed PhoPN ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare). The target protein was further purified using MonoQ and Superdex G75 columns (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: The distance between two GFP foci on chromosome IV on a single focal plane in a intact yeast cell taken by the fluorescence microscope system (Delta vision, Applied Precision) was measured by NIH image J program.
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bioRxiv - Biochemistry 2022Quote: ... Lysates from 8 L of yeast treated cells were then subjected to a 5 ml Nickel affinity purification using an AKTA purifier system (GE Healthcare) equilibrated in the urea buffer ...
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bioRxiv - Molecular Biology 2023Quote: The binding of anti-RBD antibody in post-immunization mice sera to the yeast purified RBD was analysed by SPR using a Biacore T200 system (GE Healthcare) at 25°C ...
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bioRxiv - Biochemistry 2023Quote: The DNA sequences encoding the DNA binding domains of Pdr1p (residues 1-207) and Pdr3p (residues 1-184) were amplified by PCR from the Yeast Genomic Tiling Collection (GE Healthcare YSC4613). The PCR products were inserted into the pMCSG7 vector by ligation independent cloning ...
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced by adding (isopropyl β-D-1-thiogalactopyranoside, IPTG; GE Healthcare) to a final concentration of 0.3 mM ...
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bioRxiv - Microbiology 2021Quote: ... was engineered into the MCS of the pGEX4T2 expression vector (GE Healthcare Life Sciences) using BamHI and XmaI restriction sites ...
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bioRxiv - Plant Biology 2021Quote: ... using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (IDT ...
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bioRxiv - Neuroscience 2023Quote: ... Protein expression was detected after 3 min incubation with a chemoluminescent substrate (GE Healthcare RPN2106 for GAPDH ...
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bioRxiv - Cell Biology 2020Quote: ... Localization of mCherry- and GFP-tagged fusion proteins was performed by fluorescence microscopy of live yeast cells using a Visitron spinning disk CSU-W1 (Visitron Systems, Puchheim Germany) or a DeltaVision deconvolution microscope (Applied Precision, Issaquah, WA). The Visitron spinning disk CSU-W1 consisted of a Nikon Ti-E inverted microscope ...
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bioRxiv - Microbiology 2022Quote: ... brasiliensis by co-cultivation with yeasts at a ratio of 1:10 in sterile Hybond N filters (GE Healthcare Life Science, Pittsburgh, PA) in IM solid medium (induction medium ...
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bioRxiv - Microbiology 2021Quote: ... Protein expression induction and purification was carried out similarly with Glutathione Sepharose 4B (GE Healthcare) beads ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein expression was visualized with an Amersham ECL Western Blot System (GE Healthcare Life Sciences). β-Actin served as a loading control ...
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bioRxiv - Neuroscience 2024Quote: ... Protein expression levels were visualized with ECL Plus Western Blotting Detection Reagents (GE Healthcare Biosciences).
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bioRxiv - Plant Biology 2022Quote: ... The genes were inserted in a modified version of the pGEX-3X expression vector (GE Healthcare) using the Golden Gate DNA shuffling method of Engler et al.,29 downstream of a ‘GST–factor Xa cleavage site’ coding sequence.30 The GST tag was removed by cleavage with bovine factor Xa ...
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bioRxiv - Plant Biology 2021Quote: ... G-blocks were inserted in a modified version of the pGEX-3X expression vector (GE Healthcare) using the Golden Gate DNA shuffling method of Engler et al ...
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bioRxiv - Cell Biology 2022Quote: ... and protein expression was visualized using an enhanced chemiluminescence detection system (GE Healthcare, Little Chalfont, UK) as described before88 ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified cpxA31-163 was cloned into BamHI/EcoRI-digested pGEX-6P-1 expression vector (GE Healthcare), which encodes for an N-terminal glutathione S-transferase (GST ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lc3b was constructed by cloning Lc3b into the BamHI and SalI sites of pGEX-4T-1 (GE Healthcare, IL, USA). The pac sequence was amplified from pBApo-EF1α Pur and cloned into pBApo-EF1α Neo ...