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Citations for GE Life Sciences :
1 - 50 of 521 citations for WY 45494 hydrochloride CAS 93413 90 2 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... 2 mM EDTA) for 90 minutes at 4° Celsius and then scanned and examined by Typhoon trio+ (GE Healthcare) scanner using Cy5 channel.
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bioRxiv - Neuroscience 2023Quote: ... 11.5 mL of 90% Percoll (GE Healthcare) diluted in 10X PBS (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and ethanolamine hydrochloride (EA) were obtained from GE Healthcare/Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... and ethanolamine hydrochloride (EA) were obtained from GE Healthcare/Cytiva (Marlborough ...
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bioRxiv - Biophysics 2024Quote: ... Samples were run for 2 h at 90 V and then scanned using a Typhoon FLA 9500 laser scanner (GE Healthcare) at a pixel size of 50 µm ...
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bioRxiv - Microbiology 2021Quote: ... and the pellet re-suspended in 90% Percoll (GE Healthcare) and overlaid with a 70% ...
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bioRxiv - Immunology 2024Quote: ... + 3 ml of isotonic Percoll (ISP; 90% Percoll (GE Healthcare) + 10% 1 M HBSS (Gibco)) ...
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bioRxiv - Plant Biology 2024Quote: ... Biotinylated proteins were pulled-down using Streptavidin Mag SepharoseTM (Cytiva 28-9857-99; GE Healthcare) and then submitted to on-bead digestion ...
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bioRxiv - Microbiology 2022Quote: ... For StrepII IP 25 μl Streptactin Sepharose® High Performance beads (GE Healthcare, GE28-9355-99) and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ fetal liver cells were viably stored in 90% FBS (GE Healthcare) and 10% DMSO (FisherScientific ...
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bioRxiv - Microbiology 2024Quote: ... The cell suspension was mixed with 90% Percoll (GE HealthCare, Pittsburg, PA) and centrifuged at 4° C for 15 minutes at 550 x g ...
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bioRxiv - Neuroscience 2021Quote: ... The chip was deactivated by 1 M Ethanolamine hydrochloride-NaOH (GE Healthcare Life Sciences) at a flow rate of 10 μL/min for 420 s ...
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bioRxiv - Microbiology 2020Quote: ... followed by size exclusion chromatography (Superdex 200 16/90; GE Healthcare, Chicago, IL) and washed with 0.5 M Arginine in Dulbecco’s PBS pH 7.4 (DPBS ...
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bioRxiv - Neuroscience 2020Quote: ... A 100% Percoll solution was prepared consisting of 90% Percoll (GE Healthcare, UK) and 10% 10x HBSS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and 90% fetal bovine serum (FBS; HyClone, GE Healthcare Life Sciences, Illinois, USA).
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bioRxiv - Developmental Biology 2022Quote: ... using a discontinuous 45/90% Percoll® gradient (GE Healthcare Biosciences, Uppsala, Sweden), sperm capacitation of frozen-thawed straws from a known fertile bull was performed ...
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bioRxiv - Biochemistry 2020Quote: ... 90 µl sample were injected onto a Superdeep 10/300 200pg column (GE Healthcare) coupled to a Prominence HPLC system (Shimadzu ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 90% heat inactivated HyClone™ Fetal Bovine Serum (FBS; GE Healthcare, Germany) and stored in liquid nitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... and 6 M guanidine hydrochloride (GdmCl) was carried out on PD10 desalting columns (GE Life Sciences #17085101). Reduced protein solutions were incubated with 2-fold molar excess of C5 maleimide Alexa Fluor 488 (AL488 ...
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bioRxiv - Biophysics 2021Quote: ... The resulting samples were loaded onto a Superdex 200 16:90 SEC column (GE Healthcare) pre-equilibrated with 20 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatants obtained from the previous step were incubated with equilibriated Strep-Tactin Sepharose beads (28-9355-99, GE Healthcare) for 45 min at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... then 3ml of 10:90 v/v of PBSX10:Percoll (GE Healthcare Bio-Sciences; Sigma-Aldrich) was added and the tube was gently mixed ...
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bioRxiv - Neuroscience 2020Quote: ... The cell suspension was carefully layered on top of 4 ml of 90% Percoll (GE Healthcare, Chicago ...
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bioRxiv - Biochemistry 2024Quote: ... 30μM of His-crIFT172968-C or 30μM His-TEV protease were immobilized on 50μL of TALON beads (GE healthcare #28-9574-99) by incubation at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: Subjects underwent a 90 min PET scan on a 3T Signa PET-MR scanner (GE Healthcare, Waukesha), initiated simultaneously with the start of a [11C]raclopride bolus-infusion protocol (total amount of radioactivity mean±SD 379±75 MBq ...
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bioRxiv - Immunology 2020Quote: ... hematopoietic cells were enriched by density gradient centrifugation with 40/90 Percoll gradient (GE Healthcare Life Sciences) for 30 min at 2,500 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 µg of lyophilized Aβ was resuspended in 700 µl GE buffer pH 8.5 (6 M guanidinium hydrochloride, 20 mM sodium phosphate) and loaded onto a Superdex 75 SEC column (GE Healthcare) equilibrated with aggregation buffer (20mM sodium phosphate ...
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bioRxiv - Neuroscience 2023Quote: ... The collected cell suspension was mixed with isotonic Percoll (GE Healthcare, 90% Percoll 10% 10x PBS, pH 7.2) at a final concentration of 22.5% and centrifuged at 800 g for 20 min without break at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant containing the cells was filtered through 70-μm strainers into a tube containing 90% isotonic Percoll (GE Healthcare, #17-0891-01 ...
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bioRxiv - Microbiology 2020Quote: ... RBD with AviTag was biotinylated as described previously [32] and purified by size exclusion chromatography on a Hiload 16/90 Superdex 200 column (GE Healthcare) in 20 mM Tris-HCl pH 7.4 and 150 mM NaCl.
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bioRxiv - Biophysics 2024Quote: ... and their complexes (∼ 90 µM) in apo and holo conditions were resolved on Superdex 75 (10/300) column (Wipro GE Healthcare) and absorption was recorded at 280 nm ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... pieces of equal sizes of two post-embryonic roots and two embryonic roots from two-week-old maize plants were placed next to each other onto a moist filter paper (diameter 90 mm, GE Healthcare, UK) embedded into a petri plate (90 mm diameter ...
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bioRxiv - Systems Biology 2024Quote: ... by SDS-PAGE (10 %, 15 %, 90 µg) and transferred onto nitrocellulose membranes (0.2 µm) (GE Healthcare Life Sciences, Chalfont St Giles, UK) using the semi-dry TransBlot® Turbo™ transfer system (Bio-Rad Laboratories ...
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bioRxiv - Genomics 2024Quote: ... The supernatant from the second and third digests were combined and loaded onto a 30%/35%/45%/50% Percoll discontinuous gradient consisting of 90% Percoll (GE Healthcare, #17089101) mixed with 25 mM HEPES in HBSS in the appropriate concentrations ...
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bioRxiv - Developmental Biology 2021Quote: ... at a concentration of 1 × 106 spermatozoa/mL was used following separation of spermatozoa over a discontinuous Percoll gradient (45% and 90%; (GE Healthcare, Chicago, US)) ...
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bioRxiv - Neuroscience 2024Quote: ... Thoroughly washed cells were subjected to an isotonic Percoll gradient (90% Percoll, Catalog No. 17-0891-01, GE Healthcare Life Sciences, Chicago, IL) by suspending cells in 4 mL of 1.03g/mL Percoll in GKN buffer/0.2%BSA and overlaying cell suspension on 4 mL of 1.095 g/mL Percoll in PBS/0.2%BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were separated via 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis for 90 min and transferred to polyvinylidene fluoride blotting membranes (0.2 µm pore size; GE Healthcare, Chalfont St. Giles, UK) for 2 h ...
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bioRxiv - Microbiology 2023Quote: HeLa cells grown to 90% confluency were transfected with ON-TARGETplus human Skp1 SMARTpool siRNA or non-targeting siRNA (GE Healthcare Dharmacon, Inc., Lafayette, CO) at a final concentration appropriate to the vessel size ...
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bioRxiv - Genetics 2024Quote: ... In vitro fertilization was performed with frozen-thawed semen from a Holstein-Friesian bull (Bos taurus) after selection over a discontinuous 45/90% Percoll® gradient (GE Healthcare Biosciences, Uppsala, Sweden). The mature oocytes were fertilized by incubating them with spermatozoa at a concentration of 1 × 106 spermatozoa/mL in IVF–TALP medium enriched with BSA (Sigma A8806 ...
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bioRxiv - Microbiology 2024Quote: ... 2 µMnt ssDNA (Ovio 45) and 2 mM ATP (GE Healthcare) in a 40 µL reaction solution containing 10 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... diluted 1:10,000 in 2% TBST with 2% Blocking (GE Healthcare), was applied for 1 h.
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bioRxiv - Neuroscience 2020Quote: ... pGEX-4T-2 (GE Healthcare), pGIPZ control shRNA ...
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bioRxiv - Plant Biology 2021Quote: ... 2 M thiourea (GE Healthcare), 1% DTT ...
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bioRxiv - Plant Biology 2020Quote: ... pGEX6p-2 (GE healthcare, IL) and pBE2113-GFP (Yoshida et al. ...
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bioRxiv - Cell Biology 2023Quote: ... 2% horse serum (GE Healthcare), 0.5% penicillin-streptomycin (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM UltraGlutamine (GE Healthcare, UK), BME vitamins (Sigma-Aldrich ...