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Citations for GE Life Sciences :
51 - 100 of 140 citations for UDP β L Arabinofuranose since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The sample was diluted 3-fold with dilution buffer (50 mM Tris pH 7.5, 10 mM β-mercaptoethanol) before loading onto a MonoS 5/50 GL column (GE Healthcare) with buffer A (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2023Quote: ... The proteins were induced in Escherichia coli BL21 (Gold) cells with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) for 5 hour and purified by glutathione sepharose (GE healthcare) followed by on-column TEV cleavage and finally by size-exclusion chromatography on Hiload 16/60 Superdex 75 coloumn (GE healthcare).
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bioRxiv - Molecular Biology 2023Quote: ... Pol II buffer was exchanged to CB buffer (25mM HEPES pH 7.9, 100mM NaCl, 1mM MgCl2 and 1mM β-mercaptoethanol) and concentrated with Vivaspin 50kDa MWCO (GE Healthcare). Pol II was aliquoted and snap-frozen in liquid nitrogen until the day of the experiment.
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bioRxiv - Biochemistry 2023Quote: ... the supernatant clarified from the cell lysate after overnight induction by 0.5mM isopropylthio-β-D-1-galactopyranoside (IPTG) at 20 °C was applied to pre-equilibrated 5Lml HisTrap column (GE Healthcare). After thorough wash with binding buffer (20LmM Tris–HCl pHL7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... Knockdowns of CDK1 was performed by using SMARTpool reagents (L-003224-00-0005; GE Healthcare) and ON-TARGETplus nontargeting siRNA (GE Healthcare ...
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bioRxiv - Bioengineering 2020Quote: ... CHO supernatants were flowed through columns packed with Protein L or Protein A (GE Healthcare) in 50mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was loaded on a His-Trap FF column (GE Healthcare, Chicago, I L) equilibrated with lysis buffer and washed with 3 CV lysis buffer containing 20 mM imidazole and washed again with 3 CV lysis buffer containing 50 mM imidazole ...
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bioRxiv - Cell Biology 2019Quote: ... 150 μL GIT1-CC or β-Pix-CC protein sample at 50 μM was loaded into a Superose 12 10/300 GL column (GE Healthcare) pre-equilibrated with 50 mM Tris ...
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bioRxiv - Biochemistry 2019Quote: ... and 5 mg of β-galactosidase G5635-5KU were independently applied to a Superdex 200 10/300 GL analytical gel filtration column (GE Healthcare) equilibrated in 20 mM HEPES KOH ...
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bioRxiv - Cell Biology 2022Quote: ... The Glo3 BoCCS and GRM regions and β’-COP (residues 300-604) were ligated into BamHI/ NotI sites of pGEX-6P-1 (GE Healthcare), resulting in an N-terminal GST-tagged proteins with a 3C-protease cleavage site ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was stopped by addition of 6 mM β-mercaptoethanol and mixtures were applied to a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Microbiology 2021Quote: ... Incubation using the secondary antibody α-mouse IgG (H&L)-HRP (1:10,000) (GE healthcare #NXA931) diluted in blocking buffer was performed for two hours ...
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bioRxiv - Cell Biology 2022Quote: ... and Donkey anti-rabbit IgG(H+L) antibody conjugated with HRP (GE Healthcare, NA934V, 1:10,000). Antibody signals were detected using SuperSignal West Pico Plus Chemiluminescent Substrate (Thermofisher ...
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bioRxiv - Bioengineering 2023Quote: ... The elute from Q Sepharose was loaded on to 0.20 L source 15RPC (GE Healthcare, USA) column to separate non-glycosylated and glycosylated rhEPO ...
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bioRxiv - Cancer Biology 2023Quote: ... when cells were transfected with either 100 nmol/L siRNA targeting FADS1 (siFADS1, GE Healthcare Dharmacon), or the respective negative controls (non-targeting small interfering RNA ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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bioRxiv - Biochemistry 2020Quote: ... The 2.5 ml of R-β-actin sample was desalted to remove DTT leftovers by using PD-10 (Prepacked Disposable PD-10 Columns, GE Healthcare #17085101) column pre-equilibrated with G-buffer without DTT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... PCR products were cleaned with Sephadex G-50 fine medium (70g/L; GE Healthcare, Chicago, Illinois, USA), centrifuged through a 96-well filter plate (Phenix Research ...
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bioRxiv - Physiology 2019Quote: ... and subsequently with secondary anti-mouse/ -rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Bioengineering 2023Quote: ... Three consecutives’ batches were performed in 5 L bioreactor using ReadyToProcess WAVE 25 system (GE Healthcare, USA). A single-use 10 L Cell bag™ bioreactor (working volume ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Physiology 2021Quote: ... Results were normalized to the expression of β-actin (cat#: A3854, source: mouse, 1:80,000, Sigma-Aldrich; anti-mouse IgG (GE Healthcare; dilution: 1:25,000). Immunoreactive bands were visualized with an enhanced chemiluminescence detection system (ChemiDoc Imaging System ...
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bioRxiv - Immunology 2021Quote: ... Fab and partially cleaved IgG were applied on 1-mL HiTrap Protein L column (GE Healthcare Life Science). After loading ...
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bioRxiv - Biophysics 2020Quote: The transducin heterotrimer was isolated from the rod outer segment of bovine retina (W L Lawson Company) and Gβ1γ1 was separated from Gαt with Blue Sepharose 6 Fast Flow (GE Healthcare) as described(21) ...
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bioRxiv - Biophysics 2022Quote: ... These cells were cultured in RPMI 1640 with 2.05mM L-glutamine (GE Healthcare Bio-Sciences Corp., MA, USA) supplemented by 5% v/v fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine, GE Healthcare Life Sciences SH30065.02) supplemented with 50 U/ml Penicillin-Streptomycin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were grown at 37°C and 5% CO2 in high-glucose (4.5 g/L) DMEM (GE Healthcare) supplemented with 10% fetal calf serum (Biochrom ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superdex 200 Increase 3.2/300 column (GE Healthcare) equilibrated in 40 mM HEPES pH 7.4 (4 °C) ...
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bioRxiv - Biochemistry 2021Quote: The LASV L-Cstrep protein was first injected onto a Superose 6 Increase 3.2/300 column (GE Healthcare) equilibrated at 4°C in 40 mM HEPES pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... or a Chemi-Lumi One L system (Nacalai Tesque, 07880) with a bioanalyzer (LAS4000 mini; GE Healthcare Biosciences).
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bioRxiv - Physiology 2023Quote: ... and subsequently probed with secondary anti-mouse/-rabbit IgG (H+L)-HRP conjugates (1:6000, GE Healthcare, USA). Quantification was carried out on original scanned images using Image Lab software (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Biophysics 2021Quote: ... Samples were concentrated L using a 3 kDa Vivaspin™ 20 filter column (GE Healthcare, Mississauga, ON, μ Canada). The conformational mixtures were purified via size exclusion chromatography (SEC ...
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bioRxiv - Cancer Biology 2019Quote: STAT3 siRNA knockdown utilized the ON-TARGETplus Human STAT3 siRNA kit from GEHealthcare (L-003544-00-0005, GE Healthcare). This SMARTpool siRNA contains four pooled siRNAs ...
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bioRxiv - Neuroscience 2019Quote: ... L cells (ATCC® CRL-2648) were cultured in high glucose Dulbecco’s modified Eagle medium (GE Healthcare, Piscataway, NJ, USA) plus 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... siRTN4 (L-010721-00-0005) and non-targeting Control siRNA (Cat#: D-001810-01-50) were from GE Life Sciences. 16% paraformaldehyde (Cat# ...
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bioRxiv - Microbiology 2021Quote: ... Cell debris was removed by centrifugation at 35,000g for 20 min and the supernatant from 1 l of cell culture was incubated with 2.5 ml of Glutathione Sepharose beads (GE Healthcare) at 4°C for 6 h The Glutathione Sepharose beads were then collected in a PD10 column and washed with 50 ml of PBS ...
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bioRxiv - Neuroscience 2020Quote: Protein samples concentrated to ∼1.0 g/L were injected into an HPLC-driven SEC column (Superdex 200 10/30 column, GE Healthcare) equilibrated with HBS-C buffer ...
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bioRxiv - Immunology 2019Quote: ... RPMI 1640 with L-glutamine and 25 mM HEPES (cat. # SH30255.01) and ECL reagent (cat. # RPN2106) were from GE Healthcare Life Sciences (Logan ...
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bioRxiv - Developmental Biology 2020Quote: ... Minced mammary tissues were then digested in phenol-red free Dulbecco’s Modified Eagle Medium (DMEM) (Nacalai Tesque) containing 2mM L-glutamine (GE healthcare), 1mg/ml collagenase (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed using M110-L microfluidizer (Microfluidics) and the protein was isolated using 1-mL HisTrap column (GE Healthcare). Protein was concentrated with Amicon Ultracel-10K (Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... and the monomers were isolated in 20 mmol L-1 sodium phosphate pH 8.0 with 0.2 mmol L-1 EDTA by a Superdex 30 column 26/600 (GE Healthcare, UK). The concentration of monomeric Aβ42 was calculated with using an extinction coefficient of 1 424 M-1 cm-1 for (A280−A300) ...
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bioRxiv - Bioengineering 2021Quote: HEK293A cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 2 mM L-glutamine (GE Healthcare Life Sciences), 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... ON-TARGETplus SMART Pool Non-targeting (D-001810-10) and CHMP2A (L-020247-01) siRNAs were obtained from GE Healthcare Dharmacon ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred to Amersham™ Protran® Western blotting membranes, nitrocellulose (pore size 0.2 μm, roll W × L 300 mm × 4 m, pkg of 1 ea) (GE healthcare) overnight 4 °C at 15 V ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μL at 5 mg/mL of recombinant SMCHD1 protein sample was each loaded onto a Superdex-200 5/150 (GE Healthcare) pre-equilibrated in purification buffer [200 mM NaCl ...