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Citations for GE Life Sciences :
151 - 200 of 212 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... βSyn or γSyn cDNA was inserted into modified pGEX-KG vectors (GE Healthcare), containing an N-terminal TEV protease recognition site ...
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bioRxiv - Molecular Biology 2021Quote: ... RT cDNA products were then purified (Illustra G-50 microspin columns, GE Healthcare).
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bioRxiv - Biochemistry 2022Quote: ... RT cDNA products were then purified (Illustra G-50 microspin columns, GE Healthcare).
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bioRxiv - Developmental Biology 2023Quote: ... Amplified cDNA was purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare) resuspended in a buffer consisting of 10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was generated using Illustra RT-PCR ready-to-go beads (GE Healthcare) in a two-step reaction ...
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bioRxiv - Microbiology 2020Quote: ... The tissue solution was passed through two cell sieves (100µm then 70µm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... Selected fractions containing the N protein were concentrated and loaded on two connected Superdex 200 10/300 GL size exclusion columns (GE Healthcare Life Sciences) pre-equilibrated with 20 mM HEPES ...
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bioRxiv - Bioengineering 2020Quote: ... MRI scan acquired using a Dixon imaging sequence (Table II) acquired between two and four weeks before surgery on a clinical 3T MRI (Discovery MR750, GE Healthcare, Waukesha, WI). Regions of interest (ROIs ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatants were applied to two connected Strep-Trap HP 5 ml columns packed with Strep-Tactin ligand immobilized in an agarose matrix (GE Healthcare Life Sciences) previously equilibrated with equilibration buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Pathology 2020Quote: ... The tissue solution was passed through two cell sieves (100μm then 70μm) and then layered with Ficoll®-Paque Premium (GE Healthcare, Hatfield, United Kingdom). Density gradient centrifugation was carried out at 400g for 30 min on dissociated tissue and on whole blood ...
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bioRxiv - Genomics 2023Quote: ... At least two dried blood spot (DBS) samples (50 µL per spot) were collected on Whatman 3MM filter paper (GE Healthcare Life Sciences). DBS samples were placed in plastic bags and stored at 4°C until processing ...
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bioRxiv - Molecular Biology 2021Quote: ... or digested from dCTCF or CP190 cDNA and subcloned into pGEX-4T1 (GE Healthcare) or into a vector derived from pACYC and pET28a(+ ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... SET8 and α-tubulin cDNAs were cloned into the pGEX-5X-1 (GE Healthcare) or pMalC4X (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Plant Biology 2019Quote: ... Full-length FC1 cDNA was ligated into pGEX-2T vector (GE Healthcare, Buckinghamshire, UK) using the In-Fusion HD Cloning Kit (Takara Bio ...
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bioRxiv - Molecular Biology 2020Quote: ... or digested from Pita or CP190 cDNA and subcloned into pGEX-4T1 (GE Healthcare) or into a vector derived from pACYC and pET28a(+ ...
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bioRxiv - Microbiology 2023Quote: ... cDNA of the sample and an equal amount of Cy-3-labelled (GE Healthcare) cDNA of the reference pool were then hybridized together on customized microarray chip using commercially available hybridization platform (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Plant Biology 2020Quote: ... Suspension was gently load onto two prepared falcon tubes containing equal volumes of 2X isolation buffer and Percoll™ (GE Healthcare-17-0891-02). Samples were centrifuged in a swing bucket centrifuge at 1200Xg for 10 minutes with brakes off ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified in two steps by immobilized metal chelate affinity chromatography (IMAC) using 5 mL HisTrap FF columns (GE Healthcare, Chicago, Illinois, USA) as previously described ...
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bioRxiv - Biochemistry 2022Quote: ... The resultant library was injected onto a Superose 6 10/300 column (GE Healthcare) equilibrated in TSG buffer on an AKTA FPLC ...
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bioRxiv - Immunology 2019Quote: ... Dual-indexed library molecules were then purified using Sera-Mag beads (GE Life Sciences) to select for DNA fragments larger than 150bp ...
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bioRxiv - Neuroscience 2021Quote: ... and proteins were transferred to an activated (100% Ethanol, 1 min; followed by two washing steps with water) PVDF membrane (GE Healthcare, Buckinghamshire, UK; Cat# 10600023) at 20V for 45 min ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted protein was captured by two round of batch absorption (BA) with 4 mL/L of pre-equilibrated Ni Sepharose beads (GE Healthcare, Cat #17-5318-01). The bound beads were transferred to gravity flow column and sequentially washed with 30 CVs Wash Buffer (50 mM HEPES 7.5 ...
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bioRxiv - Bioengineering 2023Quote: ... heated to 60°C to remove aggregates and further purified on two coupled IEX columns 1 mL HiTrap Q FF and 1 mL HiTrap SP FF (GE Healthcare Life Sciences, NSW, Australia) by collecting the flow-through ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was labelled individually with both Cy-3 and Cy-5 dyes (GE Healthcare) using Superscript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... The amplified cDNA was purified using 1x volume of hydrophobic Sera-Mag SpeedBeads (GE Healthcare) resuspended in a buffer consisting of 10 mM Tris ...
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bioRxiv - Systems Biology 2020Quote: ... The amplified cDNA was purified using 1x volume of hydrophobic Sera-Mag SpeedBeads (GE Healthcare) resuspended in a buffer consisting of 10 mM Tris ...
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bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
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bioRxiv - Neuroscience 2021Quote: ... was generated by subcloning the carboxy-terminal domain of CPE (aa 194-319 with two mutations Y306W and S313H) via the EcoRI and SalI restriction sites of the pGEX-4T1 (GE Healthcare, cat. #28-945-9545-49) plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
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bioRxiv - Immunology 2021Quote: ... The amplified cDNA was purified using 1x volume of Sera-Mag SpeedBeads (GE Healthcare, South Logan, USA) resuspended in a buffer consisting of 10 mM Tris (Applichem ...
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bioRxiv - Biochemistry 2021Quote: ... cDNAs were separated by 8% TBE-urea gels and visualized by Typhoon™ FLA 9000 (GE Healthcare). Sequencing ladders were made using the Sequenase 2.0 DNA Sequencing Kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Template cDNAs were described in previous publications (15,18,19) or purchased from Open Biosystems (Dharmacon/GE Life Sciences) as part of the Mammalian Gene Collection.
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bioRxiv - Biochemistry 2022Quote: The cDNA of human full-length SMN and truncations were inserted in pGEX-6P-1 (GE Healthcare) using BamHI and XhoI ...
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bioRxiv - Microbiology 2023Quote: ... Cy5-labeled cDNAs were detected by fluorescent mode using a Typhoon FLA 9500 (GE Healthcare Life Sciences) Gel Scanner and LPR Ch.2 filter and 635 nm laser.
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bioRxiv - Microbiology 2021Quote: ... The amplified wild type and mutant PDE12 cDNAs were cloned into plasmid pGEX-6P-1 (GE Healthcare, USA) at the EcoRI and SalI restriction site ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant containing cDNA-linker-VHH complex was purified using His Mag Sepharose Ni beads (GE Healthcare, USA). The supernatant was added to 20 μL of the beads and incubated at 25°C for 30 min ...
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bioRxiv - Bioengineering 2019Quote: ... cDNA was synthesized from the purified RNA (kit Ready-To-Go You-Prime First-Strand Beads (GE Healthcare®)).The synthesized cDNA ...
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bioRxiv - Microbiology 2019Quote: ... The resulting RNA and cDNA quality was evaluated by the absorbance ratio (A260/A280 ratio) (NanoVue Spectrophotometer, GE Healthcare Bio-Sciences ...
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bioRxiv - Molecular Biology 2022Quote: cDNAs were PCR-amplified using corresponding primers (Supplementary Table S3) and cloned into a modified pGEX4T1 vector (GE Healthcare) encoding the TEV protease cleavage site after GST and into the vector derived from pACYC and pET28a(+ ...
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
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bioRxiv - Cell Biology 2019Quote: ... was used for cDNA synthesis using the Ready-to-Go You-Prime It First-Strand beads and random primers (GE Healthcare). RT-PCR reactions were conducted using the GoTaq qPCR Master Mix (A6001 ...
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bioRxiv - Plant Biology 2020Quote: ... The solution was neutralized by adding 1 M HCl and the cDNA was purified with the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare). The 5’-3’ junction sequence was amplified by PCR with cox1 specific primers Atcox1-5’(−176..-196 ...
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bioRxiv - Microbiology 2020Quote: ... Sequence verified human (clone IMAGE:5309813) and mouse (clone IMAGE:30060666) cDNAs for PDGFRβ were obtained from the Dharmacon Mammalian Gene Collection (GE Healthcare) for creation of surface-expressed GFP fusion receptor constructs ...
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bioRxiv - Cell Biology 2021Quote: ... Full-length human αSyn cDNA containing an N-terminal myc epitope-tag was inserted into a modified pGEX-KG vector (GE Healthcare), after a TEV protease recognition site to allow cleavage from GST (myc-αSyn contains an extra N-terminal glycine after cleavage with TEV protease) ...
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bioRxiv - Genomics 2019Quote: ... was used for first-strand cDNA synthesis by reverse transcription with oligo(dT)18 using Ready-To-Go You-Prime First-Strand Beads (GE Healthcare) in a final volume of 20 μL ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Biochemistry 2022Quote: ... were obtained as synthetic cDNA codon optimized for Escherichia coli expression and cloned into the pGil-MBP [38] and pGex-6P3 (GE Healthcare) as previously described [30] ...