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Citations for GE Life Sciences :
151 - 200 of 751 citations for Total Phenols Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Quantification of total and individual subline cell numbers was performed either using custom protocols in Developer Toolbox 1.7 software (GE Healthcare) or CellProfiler (Carpenter et al. ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins were separated in a 4–15% SDS–PAGE gradient and transferred to nitrocellulose membrane (GE Healthcare Life Science). Membranes were blocked with 5% (m/v ...
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bioRxiv - Plant Biology 2021Quote: ... Approximately 1 mg of total cytosolic proteins was injected onto a Superdex® 200 10/300 GL column (GE Healthcare). The SEC elution was performed with the mobile phase [50 mM Hepes/KOH ...
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bioRxiv - Plant Biology 2023Quote: ... first-strand cDNA was synthesized from 2 µg of total RNA using Ready-to-Go RT-PCR beads (GE healthcare) and an oligo(dT ...
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bioRxiv - Molecular Biology 2023Quote: 10 μg of total RNA were separated on denaturing 1.2% agarose gel and then transferred to Hybond-XL nylon membrane (GE Healthcare). 32P-labelled oligonucleotides (listed in Table S4 ...
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bioRxiv - Neuroscience 2023Quote: ... Unbound iron was separated from the total complex using PD midiTrap-G25 columns following manufacturer’s instructions (GE Healthcare Bio-Sciences).
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bioRxiv - Molecular Biology 2023Quote: ... 23S rRNA was purified from total RNA by size-exclusion chromatography using a Superose 6 10/300 column (GE Healthcare) eluted at 0.4 mL/min with 300 mM ammonium acetate ...
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bioRxiv - Biochemistry 2024Quote: ... The eluate was concentrated to a total volume of 2.5 mL and desalted using PD-10 desalting columns (GE Healthcare) and desalting buffer (25 mM Tricine-NaOH ...
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bioRxiv - Cell Biology 2020Quote: ... GST kit (GE Healthcare, Chicago, IL) was used for purifying GST-Rheb following manufactures instructions and eluted with reduced glutathione ...
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bioRxiv - Cell Biology 2019Quote: ... ECL chemiluminescent kit (RPN2132, GE Healthcare) was used for detection according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... A His-capture kit (GE Healthcare) was used to immobilize anti-His antibodies on CM5 chips (as recommended by the manufacturer) ...
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bioRxiv - Cancer Biology 2019Quote: ... The His SpinTrap Kit (GE Healthcare) was used according to manufacturer’s recommendations to purify the recombinant CELF1 proteins ...
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bioRxiv - Cell Biology 2019Quote: ... The ECL Prime Kit (GE Healthcare) was used for HRP detection.
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bioRxiv - Microbiology 2019Quote: ... then immediately rebuffered in 3.5 mL assay buffer (sodium citrate buffer 50 mM, pH 6) in a PD-10 gel filtration column (GE Healthcare) and concentrated to a final volume of 500 µL in a 3 kDa MWCO Vivaspin centrifugal filtration unit (Sartorius) ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation assays were performed using nProtein A Sepharose 4 Fast Flow or nProtein G Sepharose 4 Fast Flow (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: The recombinant proteins purified as described above were subjected to gel filtration assay (Superdex 200 Increase10/300 GL; GE Healthcare) in buffer containing 20 mM Tris (pH 8.0) ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration in the lysates was determined by Bradford assay against BSA and 1-3mg protein-lysate equivalent was loaded onto 10μL of bead slurry (Dynabeads, GE healthcare) prewashed twice with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: Proteins (30 to 50μg of total cell lysates) were separated in SDS-PAGE gels and transferred to PVDF membranes (Amersham Hybond-P, GE Healthcare). Membranes were then blocked in PBS-T (PBS – 0.1% Tween 20 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 10 μg total RNA was separated on 8 % PAGE/7 M Urea and electroblotted to Hybond N+ nylon membranes (GE Healthcare). After UV crosslinking ...
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bioRxiv - Genetics 2020Quote: ... The cap-enriched mRNA was prepared from 100 μg of total RNA with GST-eIF4EK119A protein and glutathione sepharose 4B (GE Healthcare), as described previously (Bilska et al. ...
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bioRxiv - Plant Biology 2021Quote: Total RNA (10 µg) was electrophoresed on a 1% formaldehyde denaturing gel and transferred onto the Hybond N+ membrane (GE Healthcare). The BrGypsy ...
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bioRxiv - Immunology 2020Quote: ... washed 4 times in PBS and imaged in PBS with total internal reflection fluorescence (TIRF) mode in DeltaVision OMX Imaging System (GE Healthcare). TIRF images of cortical actin and pTyr were processed with ImageJ macro using B220 and bright-field channels to discriminate between attached WT or MIM-KO cells ...
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bioRxiv - Cell Biology 2021Quote: ... The lysates with equal total protein amount were incubated with 50 μl pre-washed Glutathione Sepharose 4B (GE Healthcare Life Sciences) in 100 μl GST pull-down buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: ... total protein (50 μg) was subjected to SDS-PAGE followed by transfer to polyvinylidene difluoride membrane (GE Healthcare Life Sciences, Europe) using a semidry trans-blotter (BioRad ...
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bioRxiv - Genomics 2019Quote: 10 μg of total RNA were loaded on denaturing 1.2% agarose gel and transferred to Hybond™-XL nylon membrane (GE Healthcare). 32P-labelled oligonucleotides (see Table S4 ...
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bioRxiv - Neuroscience 2022Quote: ... 1 mg of total protein was pre-cleared for 3h at 4°C by incubation with glutathione-coupled sepharose beads (GE Healthcare) previously equilibrated in Wash buffer 1 (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... 6 μg of total RNAs were resolved on a 1 % (w/v) agarose gel then transferred onto Amersham Hybond-N+ Membrane (GE Healthcare) by capillary transfer ...
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bioRxiv - Cell Biology 2022Quote: ... A total of 15 raw images (five phases, three rotations) per plane were collected and reconstructed by using SoftWoRx software (Applied Precision), processed in ImageJ/Fiji (Schindelin et al. ...
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bioRxiv - Cell Biology 2022Quote: ... were acquired every 1–3 min over a total time period of 4–6 h and deconvolved using the softWoRx software (Applied Precision). In addition ...
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bioRxiv - Physiology 2022Quote: ... Equal amounts (30 μg) of total protein were separated by 10 % SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto nitrocellulose membranes (GE Healthcare). After blocking with Tris-Buffered Saline (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were seeded at ∼ 7.93 × 108 total viable cells (around 7.93 × 105 viable cells/mL) in a chemically defined media (ActiPro media; GE Healthcare, USA). The cultures were maintained with setpoints for pH at 7.2±0.05 ...
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bioRxiv - Biochemistry 2022Quote: ... injected (5 mL total) into HiLoad 16/600 Superdex 75pg (Cytiva) connected to an AKTA FPLC system (GE Healthcare Life Science). Gel filtration was performed using the same buffer to purify Nurr1-RXRα LBD heterodimer for analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Erythroblasts were isolated when reaching 60-70% of the total cell population by gradient centrifugation at 1000 x g for 20 minutes at room temperature over Percoll (GE Healthcare). Isolated erythroblasts were frozen in FBS containing 10% DMSO at -80°C ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 35 μg protein from each sample was resolved by SDS-PAGE and transferred to a nitrocellulose membrane (GE Healthcare). The following antibodies were used at the indicated dilutions ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was extracted and from cell monolayers using a magnetic bead-based protocol using Sera-Mag SpeedBeads™ (GE Healthcare). Isolated RNA was DNase-treated with RQ1 DNase (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... 200 μg of total protein extracts were fractionated in a 10 % SDS-PAGE followed by a wet transfer to a PVDF membrane (GE Healthcare) [78] ...
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bioRxiv - Molecular Biology 2024Quote: ... Concentrated FhES (10 mg total protein) was fractionated (2 mL/fraction) by SEC on a Sephacryl S-200 HR 26/60 column (GE Healthcare) connected to an AKTA Start automated liquid chromatography system (GE Healthcare) ...
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bioRxiv - Biochemistry 2023Quote: ... After staining with Ethidium bromide to visualize total DNA and gel transfer on a Nylon N+ membrane (GE Healthcare Ref RPN203B) (Qbiogen ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was extracted using the kit Illustrative Bacteria Genomic Mini Spin Kit (GE Life Sciences, Switzerland) according to manufacturer’s recommendations ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We developed an in vitro assay using various masses and distributions of inert glass microfiber discs (GE Healthcare, Life Sciences, USA) to assess the decision-making power of Physarum polycephalum ...
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bioRxiv - Bioengineering 2019Quote: Product quality assays were performed on samples purified by Protein A chromatography using MabSelect SuRe LX resin (GE Healthcare, Amersham, UK). The column was equilibrated in a citrate/sodium chloride buffer at pH 7 ...
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A GID E3 ligase assembly ubiquitinates an Rsp5 E3 adaptor and regulates plasma membrane transportersbioRxiv - Cell Biology 2021Quote: ... The outcome of the activity assay was visualized with a fluorescent scan of an SDS-PAGE gel with Typhoon imager (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... For the in vitro ATPase activity assay and pull-down assay the buffer of the proteins was exchanged via a PD SpinTrap G-25 spin columns (GE Healthcare) to activity assay buffer (25mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The elution fractions showing the highest protein concentration in a Bradford-assay (Bradford, 1976) were pooled and purified with size-exclusion chromatography using PD10 desalting columns (GE Healthcare) and buffer B (100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... was incubated 2 h at a dilution of 1:10,000 and revealed by ECL Prime colorimetric assay (GE Healthcare, Chicago, USA).
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bioRxiv - Immunology 2022Quote: For in vitro assays PBMCs were isolated using density gradient centrifugation with Ficoll-Paque PLUS (GE Healthcare Bio-Sciences AB, Sweden). Heparinized blood was diluted with phosphate-buffered saline (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... The percent monomer was determined by dividing the estimated area under the monomer peak (elution volume of ∼15.4 to 17 mL) with the total area (monomer + dimer) using UNICORN software (default software of AKTA FPLC, GE Healthcare Life Science), followed by multiplication by 100.
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bioRxiv - Immunology 2020Quote: Total IgG1 antibodies were captured from 2 µL of serum using Protein G Sepharose 4 Fast Flow beads (GE Healthcare, Uppsala, Sweden) in a 96-well filter plate (Millipore Multiscreen ...
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Cell Biology 2022Quote: ... The intensity of each band was quantified as a percentage of total lane signal using Image Quant TL 8.1 software (GE Healthcare Life Sciences). Oligonucleotides used are listed in Sup ...