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Citations for GE Life Sciences :
51 - 100 of 863 citations for Toscana Virus Glycoprotein 2 Gc Human Heterodimeric Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Fc-Fusion proteins were isolated using HiTrap protein-A (GE Healthcare) affinity columns.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare Life Sciences) and 1% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS) (GE Healthcare Life Sciences), 10 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (v/v) foetal calf serum (FCS, Gibco or GE Healthcare) and 100 U/ml penicillin and 100 µg/ml streptomycin (P/S ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal calf serum (FCS, GE Healthcare and Eurobio), pyruvate sodium 1mM (Life Technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal His6 tag was removed by digestion with thrombin (GE Healthcare) by adding the enzyme at a concentration of 10 Units per mg of KsgA protein during overnight dialysis against PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Antigen B was further purified from Fc using rProteinA Sepharose (GE Healthcare), followed by deglycosylation with endoH (New England Biolabs) ...
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bioRxiv - Genetics 2021Quote: SARS-CoV-2 receptor binding domain binding to human extracellular ACE2 were analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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bioRxiv - Cell Biology 2024Quote: Human HeLa (ATCC® CCL-2™) were cultured in DMEM (PanEco) supplemented with 10% fetal bovine serum (FBS; HyClone/GE Healthcare) and penicillin/streptomycin ...
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bioRxiv - Immunology 2022Quote: ... Tags were removed by Enterokinase treatment and separated by HiTrap SP HP (GE Healthcare).
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bioRxiv - Immunology 2019Quote: ... Chimeras bearing tandem affinity purification tags were captured using streptavidin-sepharose beads (GE Healthcare). SDS-PAGE gels were transferred to either Immobilon-P (EMD Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Molecular Biology 2022Quote: ... The CobB protein was separated from His tag on His-Trap column (GE Healthcare), concentrated at 4 °C to 6 mg ml-1 ...
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bioRxiv - Biochemistry 2019Quote: ... The tag-free proteins were further purified by anion exchange chromatography (MonoQ; GE Healthcare). Peak fractions were collected ...
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bioRxiv - Immunology 2021Quote: ... and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare), followed by size-exclusion chromatography on TSKgel G4000SWXL (TOSOH ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was removed using PreScission protease (GE Healthcare Life Sciences (now Cytiva)) ...
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bioRxiv - Biochemistry 2023Quote: ... The GST-tag was removed by an on-column cleavage using thrombin (GE Healthcare) at a final concentration of 1 unit/mL ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS; GE Healthcare Bio-Sciences, Chicago, IL), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fused ACE2 proteins were purified on HiTrap Protein A column (GE Healthcare) and eluted with 50 mM Tris pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
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bioRxiv - Microbiology 2022Quote: ... Virus stocks were cleared of residual EdC using a PD-10 desalting column (GE Healthcare, Mississauga, ON) utilizing the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The virus-inactivated RPC eluate was loaded on to 0.10 L Macrocap SP Sepharose (GE Healthcare, USA) column to remove RPC buffers and rhEPO aggregates followed by reconstitution into pre-formulation buffer ...
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bioRxiv - Biophysics 2019Quote: ... The 12xHis-tag was removed by overnight digestion with 3C protease (GE Healthcare Life Sciences) followed by a purification step with Ni-NTA agarose (QIAGEN ...
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bioRxiv - Cell Biology 2020Quote: ... The tag-free protein was reapplied onto a HisTrap HP 5 ml column (GE Healthcare) to separate the cleaved product from the respective tags and potentially uncleaved protein ...
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bioRxiv - Bioengineering 2021Quote: ... and purified by his tag affinity to a nickel column (Histrap, GE healthcare, Chicago, Illinois) via fast protein liquid chromatography (FPLC) ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tag was removed upon incubation of the target protein with thrombin (GE Healthcare) at room temperature for 16 h ...
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bioRxiv - Microbiology 2020Quote: ... The KLKs were isolated via the 6 × His tag using nickel resin (GE Healthcare, Poland) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... The tag-removed protein was loaded to a Resource Q anion-exchange column (GE Healthcare) and eluted with a linear NaCl gradient (10 mM to 300 mM) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was cleaved from the GST tag by incubation with PreScission protease (GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Tag-removed proteins were further purified by size-exclusion chromatography (Superdex 75; GE Healthcare Lifesciences). Two functional variants of SR proteins used in our experiments were the RNA binding domain (RBD ...
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bioRxiv - Immunology 2023Quote: ... that had encoded an N-terminal His6-tag followed by a PreScission protease (GE Healthcare) cleavage site ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Microbiology 2020Quote: ... lymphocytes from the lungs of influenza virus infected mice were enriched using Ficoll-Hypaque (GE Healthcare Life Sciences) density gradient and stained with H-2Db restricted tetramers conjugated to fluorophore R-phycoerythrin (PE ...
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bioRxiv - Immunology 2019Quote: ... His10-tag free CD80 ectodomain was purified with a StrepTrap HP column (GE Healthcare, catalog 28907547) in 100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The GST tag was cleaved overnight at 4°C by addition of PreScission Protease (GE Healthcare), and the BRPF1 bromodomain was eluted with wash buffer ...
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bioRxiv - Neuroscience 2021Quote: ... was cloned into the pGEX-6P-1 vector with an N-terminal GST tag (GE Healthcare).