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Citations for GE Life Sciences :
251 - 300 of 876 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed using a French press and recombinant proteins were purified by nickel ion affinity chromatography (HisTrap HP, 1ml - GE Healthcare). The His6-SUMO tag was removed using Ulp1 protease (Li and Hochstrasser ...
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bioRxiv - Microbiology 2020Quote: ... the recovered recombinant proteins were further purified by size exclusion chromatography using a Sephacryl S-400 16/60 column (GE Healthcare). Fractions containing the protein were pulled ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
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bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
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bioRxiv - Microbiology 2019Quote: ... Blots were UV cross-linked and baked at 80°C for 2 h prior to pre-hybridization at 42°C for 1 h in Rabid-Hyb Buffer (GE Healthcare Life Sciences). Hybridization with α-32P-ATP end-labeled DNA oligo probes was performed at 42°C for 2-16 h ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from donkey) (1:10,000 in 3% skim milk/TBST, NA9340, GE Healthcare) Mouse TrueBlot ULTRA ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from sheep) (1:10,000 in 3% skim milk/TBST, NA9310, GE Healthcare) ECL anti-rabbit IgG ...
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bioRxiv - Biochemistry 2019Quote: ... Crosslinked peptide fragments were enriched by SEC using a Superdex Peptide PC 3.2/30 column on an AKTA pure system (GE Healthcare) at a flow rate of 50 μL/min ...
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bioRxiv - Biophysics 2019Quote: ... the modified f(Ab)1 fragment was purified and separated from unbound dye through a gel filtration column (GE Healthcare illustra NAP Columns from Fisher ...
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bioRxiv - Biophysics 2020Quote: The binding affinities of the Fab antibody fragment Fab705 for full-length MgtE were measured using MicroCal iTC200 (GE Healthcare). The full-length MgtE proteins were purified by a similar procedure to that described above but with SEC buffer [buffer B containing 0.05% (w/v ...
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bioRxiv - Microbiology 2023Quote: ... The fragments were separated on 0.8% agarose gel and transferred by capillarity to the Amersham Hybond N+ membrane (GE Healthcare). DNA hybridization was performed with the Amersham AlkPhos Direct Hybridization Buffer ...
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bioRxiv - Microbiology 2022Quote: ... were collected by vacuum filtration onto glass microfiber filters (grade GF/C, 47 mm in diameter, pre-combusted at 460°C for >8 h, GE Healthcare Whatman, Buckinghamshire, UK), immediately plunged into liquid nitrogen ...
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bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant Mt RnhC was concentrated and further purified by size exclusion chromatography using a 16/600Superdex® 200 pg columns (GE Healthcare). Mt RnhC eluted in a single peak and the fractions were pooled ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
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bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
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bioRxiv - Biophysics 2021Quote: ... at 4°C using AKTA Pure HPLC system (GE Healthcare). The column was washed with 20 mM NaPi (pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: ... Gels were transferred onto Hybond-C nitrocellulose membrane (GE Healthcare). Membranes were blocked in 4% milk for 1 hour and incubated overnight at 4°C with shaking in the appropriate primary antibody against Nischarin (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... proteins were transferred to Nitrocellulose membrane (Hybond-C, GE healthcare) in transfer buffer using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 20°C without break in a 30% Percoll (GE Healthcare) solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Hybond-C Extra membrane (RPN203E) was purchased from GE healthcare. ECL Prime (RPN2236 ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C using a Deltavision core microscope (Applied Precision) equipped with a CoolSnap HQ2 CCD camera ...
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bioRxiv - Physiology 2023Quote: ... transferred to a nitrocellulose membrane Hybond-C Extra (GE Healthcare) in transfer medium (0.025MTris ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
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bioRxiv - Genomics 2020Quote: ... Ribosomes were treated with 60 U TruSeq Ribo Profile Nuclease to generate ribosome-protected fragments and isolated via size-exclusion with an Illustra MicroSpin S-400 HR column (GE healthcare). Ribosomal RNA was depleted from both ribosome-protected fragments and RNA-seq libraries using the Illumina Ribo-Zero Gold Kit (Human/Mouse/Rat ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid pGEX-dosR expressing DosR with an N-terminal GST-tag was generated by cloning PCR-derived dosR ORF fragment between BamHI and XhoI sites of pGEX 4T-1 (GE Healthcare) as described for pGEX-phoP (49) ...
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bioRxiv - Microbiology 2019Quote: ... sidI was amplified using primer pairs SidIBamHI-F/SidINotI-F and cloned as a BamHI/NotI fragment into pGEX-6P-1 (GE Healthcare) and pT7HMT (55) ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were treated with RNase I (5U/OD260) and ribosome-protected fragments were isolated via centrifugation through Microspin S-400 HR columns (GE Healthcare) and purified using the RNA Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2020Quote: ... HSF2BP and ARM proteins were loaded in the presence and absence of the BRCA2 fragment F15X on a Superdex 200 10/300 GL (GE Healthcare) using a HPLC Shimadzu system coupled to MALS/QELS/UV/RI (Wyatt Technology) ...
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bioRxiv - Immunology 2022Quote: ... the flow-through containing Fab fragments was buffer exchanged to TBS using Vivaspin filters with a 10 kDa molecular weight cutoff (GE Healthcare).
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Developmental Biology 2022Quote: ... Obtained antisera were purified by affinity chromatography on a column with purified Nid2a or Hmcn1 fragment coupled to CNBr-activated Sepharose (GE Healthcare). Specific antibodies were eluted with 3M KSCN ...
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bioRxiv - Biochemistry 2021Quote: ... a PCR fragment containing ubiquitin gene and a PCR fragment containing rat parkin residues 77-465 were introduced into the BamHI/XhoI sites of pGEX-6P1 (GE Healthcare). The serine (S77 ...
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bioRxiv - Cell Biology 2020Quote: ... A probe was generated from a PCR fragment homologous to the 3’ sequence just outside of the targeted region using the AlkPhos direct labelling kit (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length nsp1 was synthesized as a gene fragment from Integrated DNA technologies (IDT) and cloned into the EcoR1 restriction site of pGEX-6P-2 (GE healthcare) using in-fusion cloning (TakaraBio) ...
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bioRxiv - Cell Biology 2023Quote: ... were used at a dilution of 1:10,000 and the HRP-linked sheep F(ab’)2 fragment to mouse IgG (GE Healthcare, NA9310) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Microbiology 2023Quote: ... In separate reactions the resulting PCR fragments were coupled with an alkaline phosphatase enzyme using the Amersham Gene Images AlkPhos Direct Labelling and Detection System (GE Healthcare), according to the manufacturers protocol ...
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bioRxiv - Genomics 2023Quote: ... The digested DNA fragments were separated by electrophoresis using 1% agarose gels and transferred overnight to Hybond-N+ membranes (GE Healthcare) in 20 × SSC ...
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bioRxiv - Cell Biology 2023Quote: ... was used at a dilution of 1:10,000 and the HRP-linked donkey F(ab’)2 fragment to rabbit IgG (GE Healthcare, NA9340) was used as the secondary antibody at a 1:10,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... and ion exchange of the excised fragment by HiTrap DEAE (147-bp ‘601’, 185-bp ‘601’, and MMTV; Cytiva) or mono-Q (NLE- trimer ‘601’; GE Healthcare) according to established protocols(Dyer et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Pek1 and Atf1 were generated by PCR of the corresponding gene fragments from yeast genomic DNA or cDNA and cloned in frame into expression vectors pGEX-4T-1 (GE Healthcare) or pMAL-c2x (New England Biolabs) ...
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bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
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bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...