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Citations for GE Life Sciences :
1 - 50 of 691 citations for TGFB3 Human Mouse Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse or rat IgG was purchased from GE Healthcare.
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The secondary antibodies were HRP-linked antibodies against rat or mouse IgG (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Horseradish peroxidase-coupled secondary antibodies (anti-mouse and anti-rabbit, GE Healthcare; anti-rat, Jackson Immunoresearch) in combination with Clarity Western ECL Substrate (Biorad ...
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bioRxiv - Microbiology 2020Quote: ... a CM5 sensor chip was immobilized with a mouse anti-human IgG CH2 mAb using reagents and instructions supplied with the Human Antibody Capture Kit (GE Healthcare) in order to capture RBD-Fc or ACE2-Fc ...
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bioRxiv - Neuroscience 2019Quote: ... full-length cDNAs (Human SOX5, clone ID 30343519; mouse Rarb, clone ID 30608242; mouse Rxrg, clone ID 5707723; all purchased from GE Healthcare) were inserted into pCAGIG vector (Addgene plasmid # 11159) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBST and incubated with HRP-conjugated anti-rat or anti-mouse (1:2000; GE healthcare) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary HRP-conjugated antibodies were purchased from Promega or GE Healthcare (anti-mouse Cat. No. W4021, Promega; anti-rabbit Cat. No. W4011, Promega; anti-rat Cat. No. NA935V, GE Healthcare). All secondary antibodies were diluted in 5% milk-PBS with 0.3% Tween 20 ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Neuroscience 2020Quote: ... HepG2 and HEK293 cells were cultured in Dulbecco’s high glucose modified Eagle medium (DMEM, GE Healthcare) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Pathology 2023Quote: ... the membranes were incubated with secondary antibody coupled with Horseradish Peroxidase (anti-rabbit, anti-rat or anti-mouse, Amersham, GE Healthcare, 1/3000) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti-rat IgG-HRP (GE Healthcare, UK), anti-rat IgG-HRP (Cell signaling ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Immunology 2021Quote: ... Human and mouse GSDMD were further purified by gel filtration chromatography using a Superdex200 (16/60 GL) column (GE Healthcare) in a running buffer containing 20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... each well in the plate was further incubated with the HRP-sheep anti-mouse or anti-human secondary antibody (1:5000, GE Healthcare) for 1 hour at 37°C ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: ... Biacore Series S CM5 sensor chip was immobilized with a mixture of two monoclonal mouse anti-Fab antibodies (Human Fab capture kit from GE Healthcare) to capture antibodies for binding measurements ...
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bioRxiv - Cell Biology 2022Quote: ... Bound proteins were visualized using rat anti-HA antibody and detected using peroxidase-labeled anti-rat IgG antibody and ECL Plus (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... was cloned into mouse IgG1 or human IgG1 Fc backbone in pKN293E expression vectors and transiently transfected into HEK293 cells followed by media collection and purification using MabSelect SuRe antibody purification resin (Cat: 29-0491-04, GE Healthcare). SEC-HPLC and SDS-PAGE were used to check the size and purity of these recombinant proteins.
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bioRxiv - Biochemistry 2020Quote: The recombinant catalytic domains of MMP-2 and -9 were expressed in HEK293 cells stably transfected with respective constructs and purified from serum-free culture medium using Gelatin Sepharose 4B (GE Healthcare) as described in [35 ...
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bioRxiv - Immunology 2020Quote: IgGs were expressed by transient transfection in HEK293-6E cells and purified from cell supernatants using protein A or G (GE Healthcare) as previously described (39 ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were washed three times with 1xTBST before incubating with horseradish peroxidase (HRP)-conjugated secondary antibodies (Goat Anti-Rabbit – Cell Signaling Technology, Danvers, MA, USA; Goat Anti-Mouse – Sigma-Aldrich, St. Louis, MO, USA; Goat Anti-Rat – GE Healthcare Life Sciences, Little Chalfont, UK) for 1hr at 24°C with agitation ...
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bioRxiv - Biochemistry 2021Quote: ... anti-rat IgG and anti-rabbit IgG antibodies (GE Healthcare). Blots were detected using ECL Prime Western Blotting Detection Reagents (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-linked goat anti-rat IgG pAb (#NA935V, GE Healthcare) and HRP-linked rabbit anti-goat IgG pAb (DAKO #P0449 ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: Human ileal enteroids were transduced with GIPZ lentiviral shRNA kit for human Coronin 1a (GE Healthcare Dharmacon) based on protocol adapted from Heijmans et al.17 ...
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bioRxiv - Bioengineering 2021Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated from fresh human peripheral blood using Ficoll (GE Healthcare) density gradient centrifugation ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit or anti-rat IgG-linked HRP secondary antibodies (GE Healthcare) followed by ECL (Advansta).
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Acinar-to-ductal metaplasia in the pancreas requires a glycolytic switch and functional mitochondriabioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-HRP (GE_NA934V) and anti-rat-HRP (GE_NA935V) (both GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Human peripheral blood mononuclear cells (PBMC) were separated from healthy human blood samples using Ficoll-Paque (GE healthcare) or Lymphoprep (Stem Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... human ERBB4 siRNA pool and human ERBB2 siRNA pool were purchased (Dharmacon GE Healthcare, now Horizon Discovery Ltd.). miRNA mimics ...
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Immunology 2022Quote: ... and HEK293 cell lines were obtained from Royan Institute Cell Bank and cultured in Dulbecco’s Modified Eagle Medium (DMEM, GE Healthcare Life Sciences, Pittsburgh, PA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Neuroscience 2023Quote: ... at 1:1000 and anti-rat IgG HRP secondary at 1:2500 (GE Healthcare, NA935).
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bioRxiv - Microbiology 2019Quote: ... Anti-mouse (GE Healthcare) or rabbit-HRP (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...