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Citations for GE Life Sciences :
1 - 50 of 1339 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Co-immunoprecipitation: GammaBind G Sepharose beads (50ul/sample; #17-0885-01, GE Healthcare) were washed in 1xPBS (pH7.4 ...
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bioRxiv - Biophysics 2022Quote: ... the sample was first incubated with cobalt beads (GE Healthcare) to remove BirA and SrtA which both have an N-terminal His-tag ...
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bioRxiv - Pathology 2020Quote: ... free TPEN and free Zn were further removed by gel filtration on PD MidiTrap G-10 (GE Healthcare) equilibrated with 40 mM of the metal-free HEPES-KOH ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was centrifuged at 2,000 g and 4 °C for 10 min and eventually filtered with 0.43 μm organic phase medium (GE Healthcare, 6789-0404). The column was a 100×2 mm Phenomenex Luna 3 μm NH2 (Catalog No. ...
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bioRxiv - Biophysics 2024Quote: ... to remove metal ions and polysulfane sulfur before proteins were subjected to anaerobic size exclusion chromatography (SEC) (HiLoad 16/600 Superdex; GE Healthcare) on an Äkta Purifier system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... primary antibodies were used together with horseradish peroxidase-conjugated α-mouse immunoglobulin G (1:5000, GE Healthcare) as secondary antibody ...
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bioRxiv - Molecular Biology 2020Quote: ... Free azide-PEG3-biotin was removed with MicroSpin G-25 Columns (GE Healthcare). The flow-through fraction was mixed with 300 µl of Dynabeads M280 streptavidin (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... chromatography followed by TALON Superflow (GE Healthcare, a cobalt-based IMAC resin) affinity chromatography.
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bioRxiv - Microbiology 2023Quote: ... 20 µg of anti-HA monoclonal antibodies crosslinked to protein G MicroBeads (GE Healthcare) were added to the cleared lysate and incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was loaded onto a cobalt-Talon affinity agarose resin (GE Healthcare). The target protein was eluted with lysis buffer supplemented with 250 mM imidazole and 0.5 mM EDTA ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Microbiology 2021Quote: ... The excess DBCO-NHS was removed using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The DBCO-labeled lectin product (100 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Free nucleotides were removed with a spin column (Illustra MicroSpin G-25 Columns, GE Healthcare) at 750 × g for 2 min ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded onto a 5 ml HisTrap HP cobalt column (GE Healthcare) equilibrated in buffer A ...
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bioRxiv - Physiology 2021Quote: ... The immunostaining was detected using horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (GE Healthcare, Buckinghamshire, UK; dilution: 1:5000) or anti-mouse IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... Free iodine and iodinated growth factor were separated on Sephadex G-25 columns (PD10; GE Healthcare). For column equilibrium and elution ...
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bioRxiv - Genomics 2021Quote: ... The excess DBCO-NHS was also removed by using Sephadex G-25 desalting columns (GE Healthcare Japan Co., Tokyo, Japan). The resulting DBCO-labeled lectin was then incubated at 4 °C with ten-times the molar amount of 5’-azide-modified DNA oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... The His-tagged proteins were purified by cobalt affinity chromatography using Chelating Sepharose (GE Healthcare). The proteins were eluted in buffer containing 25 mM Hepes-KOH (pH 7.4) ...
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bioRxiv - Biophysics 2020Quote: ... the free dyes were removed by centrifugation in MICROSPIN G-50 column (GE Healthcare, 27-5330-01). The labeled proteins were stored at −80°C ...
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bioRxiv - Bioengineering 2020Quote: ... Transmembrane stem cell factor (tmSCF) was then isolated using cobalt chelating column (Chelating column; GE Healthcare), and the buffer was exchanged in 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... S9.6 antibodies were purified according to standard procedures (Stork et al., 2016) by using a HiTrap Protein G HP column (GE Healthcare), followed by extensive washing with 20 column volumes of PBS and elution with five column volumes of elution buffer (0.1 M glycine-HCl ...
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bioRxiv - Cell Biology 2020Quote: ... The labeled antibody was separated from free Cy7 using a PD-mini G-25 desalting column (GE Healthcare), pre-equilibrated with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2024Quote: ... The end-labelled DNA probe was purified from free label by Sephadex G-50 spin columns (GE Healthcare), and incubated with increasing amounts of purified PhoP in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2022Quote: ... elution from the HisPur™ Cobalt Resin was applied to a 5 ml streptactin column (GE Healthcare) and washed with HBS ...
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bioRxiv - Microbiology 2023Quote: ... the labelled oligonucleotides were separated from free [γ-32P] ATP using a custom Sephadex G-25 column (GE Healthcare). Labelled nucleic acids were incubated with nucleases (Rnase A ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then applied to a HiTrap cobalt-chelating TALON crude column (GE Healthcare Bio-Sciences AB). Enzyme purification was performed according to the manufacturer’s instructions using an Äkta FPLC system ...
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bioRxiv - Immunology 2021Quote: ... Finkelman (University of Cincinnati, OH) (20) and purified from culture filtrates by standard protein G column chromatography (Amersham Biosciences/GE Healthcare, Piscataway, NJ). The hybridoma cells were grown in RPMI-1640 medium (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... anti-rabbit (1:5000, GE Healthcare) or anti-rat horseradish peroxidase–conjugated secondary antibodies (1:1000 ...
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bioRxiv - Molecular Biology 2022Quote: ... anti-rabbit (1:5000, GE Healthcare), or anti-rat horseradish peroxidase–conjugated secondary antibodies (1:1000 ...
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bioRxiv - Genetics 2020Quote: Adult male mdx5cv mice (4-6 months old) were injected in their TA skeletal muscles with cardiotoxin (10 µM) and siRNA pooled oligos (10 µg; GE Healthcare Dharmacon Inc; Lafayette, CO) containing either scrambled siRNAs or siRNAs targeting mouse Dock3 mRNA following a protocol previously established54 ...
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bioRxiv - Biochemistry 2021Quote: ... with purification of labelled protein away from free label using a desalting Superdex G-25 (PD-10) column (GE Healthcare). FRET was examined using 495 excitation for FITC-HU-A ...
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bioRxiv - Microbiology 2021Quote: ... The eluted protein was buffered exchanged into endotoxin-free phosphate buffered saline (PBS) using a sterile PD MidiTrap G-25 column (GE Healthcare). Finally ...
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bioRxiv - Biophysics 2022Quote: ... After that the labeled protein was purified from the excess of free dye by a PD SpinTrap G-25 column (GE Healthcare), divided into aliquots and stored at -80 °C.
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... The click-reaction was performed by incubation for 30 min at 20 °C in the dark with vigorous intermittent shaking and the free biotin was removed from the clicked extracts using PD MiniTrap G-25 column (GE Healthcare) equilibrated with IPD buffer ...
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bioRxiv - Microbiology 2022Quote: ... end-labelled with [γ-32P ATP] (1000 Ci nmol-1) using T4 polynucleotide kinase and purified from free label by Sephadex G-50 spin columns (GE Healthcare). Increasing amounts of purified regulators were incubated with appropriately end-labelled DNA probes in a total volume of 10 µl binding mix (50 mM Tris-HCl ...
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bioRxiv - Biophysics 2022Quote: ... Broken cells then underwent ultra-centrifugation at 142,000 x g for 60 minutes before collecting the cell free extract and incubating it with pre-equilibrated HisTrap Ni2+ resin (GE Healthcare) in a 3:1 volume ratio for 2 hours under gentle shaking ...
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bioRxiv - Bioengineering 2020Quote: ... using standard Ficoll (GE Healthcare) gradient centrifugation separation ...
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bioRxiv - Cell Biology 2020Quote: ... and Cy5 (GE Healthcare Co., Ltd) NHS esters were dissolved in Dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... anti-His (1:5000, mouse; GE Healthcare) and anti-GST (1:5000 ...
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bioRxiv - Plant Biology 2024Quote: ... α-rat-HRP (GE HealthCare, NA935V, 1:5000) in 5% skimmed milk for 1h at room temperature on a shaking device ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled protein was separated from free fluorescent dye using a gel filtration column (GE Healthcare illustra NAP-10 Columns Sephadex G-25), followed by ESI-MS analysis (Agilent 6230 TOF LC/MS ...
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bioRxiv - Biophysics 2024Quote: ... The filtered supernatant was then incubated at 4 ºC with gentle shaking at 5 rpm for 1 hour in the presence of 5 mL of HisPur Cobalt Resin (GE Healthcare). After multiple rounds of resin cleaning using Extraction Buffer supplemented with 5 mM imidazole ...
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bioRxiv - Biochemistry 2024Quote: ... DsbA-RbmB was purified from the cleared lysate by loading the supernatant onto a cobalt-charged 5 mL His-trap HF (GE Healthcare) equilibrated with high-salt TBS buffer (20 mM Tris pH 7.6 ...
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bioRxiv - Plant Biology 2020Quote: ... RDR2 and protein standards (GE Healthcare) were subjected to FPLC using a Superdex 200 10/300 GL column at 4°C using 50 mM HEPES-KOH pH7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... standard enhanced chemiluminescence reagents (GE Healthcare) were used ...
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bioRxiv - Cell Biology 2020Quote: ... HRP-anti rabbit secondary (GE Healthcare; 1:5000) and HRP-anti mouse secondary (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... and donkey anti-rabbit (GE healthcare; 1:5000).
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bioRxiv - Physiology 2021Quote: ... or anti-mouse IgG (GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... GST (goat, GE Healthcare, 27457701V, WB 1:5000), MYC (mouse ...