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Citations for GE Life Sciences :
451 - 500 of 2076 citations for Streptavidin ZAP Antibody Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... washed three times with 0.1 M cacodylate and then incubated for 3-h at RT in streptavidin HRP conjugate (1:200; GE Healthcare). Sections were visualized after 1-min and 30-sec DAB (Sigma-Aldrich)/glucose oxidase reaction ...
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bioRxiv - Immunology 2020Quote: ... Lysates were centrifuged at 15,000xg for 5 min and supernatants were incubated with 50 μL of Streptavidin Sepharose High Performance beads (GE Healthcare) for 1 h rotating ...
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bioRxiv - Neuroscience 2021Quote: ... free-floating sections were washed in PBS and incubated overnight in Cy3-conjugated streptavidin (1:1000 dilution; PA43001, GE Healthcare) in “Triton-PBS” (PBS containing 0.3% v/v Triton X-100 [Sigma]) ...
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bioRxiv - Biochemistry 2019Quote: ... Elution was performed with the lysis buffer containing 250 mM imidazol pH 8.0 and eluted proteins were directly attached on a 10 ml Streptavidin Sepharose (GE Healthcare). Proteins were extensively washed with Strep buffer (20 mM Hepes ...
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bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Biotinylated proteins were captured from lysates (500 µg per replicate) using 10 μg streptavidin sepharose (GE healthcare, Waukesha, Wisconsin, USA) for 8 h ...
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bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were isolated from cleared lysates by incubating with 70 µl of streptavidin beads (17-5113-01, GE Healthcare) at 4°C for 3h ...
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bioRxiv - Cell Biology 2022Quote: ... and membranes were incubated with secondary antibodies conjugated to horseradish peroxidase (anti-mouse or anti-rabbit; GE Healthcare 17097199 and 16951542, 1:5000 dilution) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... incubated for 1 h at room temperature with 1:10,000 dilutions of anti-rabbit or anti-mouse secondary antibodies (GE Healthcare Life Sciences, Pittsburgh, PA), washed 3X with TBS-T ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a tube containing 30 µl of pre-washed packed streptavidin beads (GE Healthcare, 17-5113-01). Streptavidin pulldown was done for 3 hours at 4°C ...
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bioRxiv - Biophysics 2019Quote: 500 resonance units (RU) of Biotinylated DCAF15-DDB1-DDA1 was immobilized to a streptavidin SA Sensor Chip (GE Healthcare Life Sciences) in a running buffer consisting of 50 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: Conversion beads were constituted of porous streptavidin-conjugated sepharose hydrogel with 34 μm average particle size (GE healthcare: GE17-5113-01) functionalized with biotinylated DNA constructs ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Neuroscience 2021Quote: ... to a final volume of 300 μl and incubated with 10 μl of medium slurry of streptavidin-coated magnetic beads (GE Healthcare) for 2 hours at room temperature under continuous rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The SPR analyses were carried out using the BIAcore 8K system with a streptavidin-coated (SA) chip (#BR-1005-30, GE Healthcare) at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... isoform 1 with N-terminal MRGS(H)8 and C-terminal Avi tag (Hismyosin VI (aa 992-1031)Avi) was immobilized on either MyOne T1 streptavidin-coated beads (Pierce) or Sera-Mag neutravidin-coated beads (GE Healthcare). The use of the type of beads was alternated during selection rounds ...
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bioRxiv - Molecular Biology 2023Quote: ... was immobilised to approximately 2100 response units (RU) on a flow cell of a Streptavidin coated (SA) sensor chip (GE healthcare) according to manufacturer’s directions while another flow cell was left blank for reference subtraction and monitoring any non-specific bindings ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Neuroscience 2023Quote: ... (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K, Na, or Li) was injected over the streptavidin-coated surface of a sensor chip SA (GE Healthcare). The amount of immobilized RNA was as follows ...
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bioRxiv - Biochemistry 2024Quote: ... or biotinylated CDO1 (bio-CDO1) was immobilized on a streptavidin chip using a Biacore T200 or a Biacore 8K (GE Healthcare). To determine the kinetics-affinity of the small molecules for proteins ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Developmental Biology 2019Quote: ... normal mouse IgG (GE Healthcare)
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG (GE Healthcare) or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... anti-mouse IgG (GE Healthcare, distributed by VWR in Ismaning ...
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bioRxiv - Developmental Biology 2020Quote: ... or anti-mouse (GE Healthcare) antibodies linked with horseradish peroxidase (HRP ...
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bioRxiv - Developmental Biology 2020Quote: ... Membranes were washed with PBST and incubated with alkaline phosphatase-conjugated secondary antibodies (anti-rabbit IgG and anti-mouse IgG, GE healthcare life sciences, 1:10000) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... the membranes were incubated for 90 minutes at room temperature with secondary antibodies (donkey anti-rabbit HRP-IgG, GE Healthacare, cat#NA934V, 1:5000 or goat anti-mouse HRP-IgG, GE Healthcare, cat#NA931V, 1:2000) diluted in blocking solution and washed as before ...
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bioRxiv - Biophysics 2019Quote: ... The membrane was rinsed three more times in TBS-Tw and incubated for 30 min with secondary antibody (HRP-conjugated sheep anti-mouse IgG, GE Healthcare Life Sciences; Freiburg, Germany) diluted 1:20,000 in TBS-Tw + 1% milk ...
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bioRxiv - Cancer Biology 2022Quote: ... 1:100 Dako #M7248) (overnight, 4 °C) and biotinylated secondary antibodies (HRP anti-mouse and HRP anti-rabbit, 1:200, GE Healthcare (2h at room temperature). Target proteins were detected with the ABC Kit and the DAB kit (Vector Laboratories).
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before incubation for 30 min at room temperature with secondary antibodies coupled to horseradish peroxidase at 1:10000 dilution in 5% milk/PBST: donkey anti-mouse (GE Healthcare NA931-1ML, Chicago, IL), donkey anti-rabbit (GE Healthcare NA934-1ML) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by an incubation with horseradish peroxidase (HRP) secondary antibodies (Mouse IgG HRP Linked Whole Ab-GE Healthcare, #NA931, 1:5000; Rabbit IgG HRP Linked Whole Ab-GE Healthcare, #NA934, 1:5000) in 3% milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... a CM5 sensor chip was covalently modified by an anti-GST polyclonal goat antibody (GST capture Kit, GE Healthcare). The surface of flow cell 1 and 2 was activated for 60 s with 50 mM NaOH (10 μL/min) ...
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bioRxiv - Biochemistry 2022Quote: ... Bound secondary antibodies detected by chemiluminescence using the ECL kit according to the manufacturer’s instructions (GE Healthcare, Uppsala, Sweden). Finally ...
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bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2019Quote: ... or anti-phospho hTERT mouse mAb (TpMab-1) and anti-mouse IgG HRP (GE Healthcare) were used for IB to detect phosphorylated hTERT proteins.
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bioRxiv - Epidemiology 2021Quote: ... CM5 chips for surface plasmon resonance (SPR) assay and Cytometric Bead Array (CBA) Mouse Immunoglobulin Isotyping Kit were purchased from GE HealthCare (Boston, USA). Ninety-six-well MultiScreen HTS plates were purchased from Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide was diluted to 50 nM in running buffer (HBS-EP) and immobilized on a streptavidin sensor chip (sensor chip SA, GE Healthcare, USA). The analytes KEAP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Microbiology 2019Quote: ... secondary antibodies conjugated with horseradish peroxidase were used for detection by chemiluminescence using the Amersham ECL Prime Kit (GE Healthcare). Biochemical fractionation of infected cells was performed as previously described [19].
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bioRxiv - Cell Biology 2022Quote: ... or anti-mouse (NA931V, GE Healthcare). These were incubated for 1 hr at room temperature and blots were developed using Enhanced Chemiluminescence (ECL prime ...
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bioRxiv - Immunology 2019Quote: ... donkey anti-mouse-HRP (GE Healthcare), donkey anti-rabbit-HRP (GE Healthcare) ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-mouse-HRP (GE healthcare, NXA931) 1:5000 and HRP Protein A (BD Biosciences ...