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Citations for GE Life Sciences :
251 - 300 of 1860 citations for Streptavidin ZAP Antibody Kit goat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 0.15 M NaCl and 0.05% Tween 20 (TNT) at 46 °C for 1 h followed by a streptavidin-Cy5 (GE Healthcare) detection step ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotinylated proteins were isolated from cleared lysates by incubating with 70 µl of streptavidin beads (17-5113-01, GE Healthcare) at 4°C for 3h ...
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bioRxiv - Cell Biology 2020Quote: ... Goat anti-mouse-HRP (GE Life Sciences #NA931, 1:5000) Ten micrograms of each sample were electrophoresed through a 12-well ...
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bioRxiv - Microbiology 2020Quote: ... Goat anti-mouse and anti-rabbit IgG-HRP (GE Healthcare) were used at 1:10000 dilution ...
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bioRxiv - Genetics 2022Quote: ... or goat-developed anti-mouse immunoglobulin G (GE Healthcare, NA931). Membranes were developed with ECL+ and signals were obtained using ChemiDoc XRS+ and analyzed by Image Lab software (Bio-Rad).
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bioRxiv - Cell Biology 2019Quote: ... and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare, NA934VS), and developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... ECL Plex goat anti-mouse IgG-Cy3 (PA43009, GE Healthcare), HRP-conjugated goat anti-rabbit antibodies (#31460 ...
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bioRxiv - Cell Biology 2022Quote: ... HRP-linked goat anti-rat IgG pAb (#NA935V, GE Healthcare) and HRP-linked rabbit anti-goat IgG pAb (DAKO #P0449 ...
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bioRxiv - Cell Biology 2023Quote: ... and HRP-linked goat anti-rabbit (1:10,000, GE Healthcare). Antibodies were incubated in 5% non-fat milk PBST (PBS with 0.1% Triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a tube containing 30 µl of pre-washed packed streptavidin beads (GE Healthcare, 17-5113-01). Streptavidin pulldown was done for 3 hours at 4°C ...
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bioRxiv - Biophysics 2019Quote: 500 resonance units (RU) of Biotinylated DCAF15-DDB1-DDA1 was immobilized to a streptavidin SA Sensor Chip (GE Healthcare Life Sciences) in a running buffer consisting of 50 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: Conversion beads were constituted of porous streptavidin-conjugated sepharose hydrogel with 34 μm average particle size (GE healthcare: GE17-5113-01) functionalized with biotinylated DNA constructs ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Neuroscience 2021Quote: ... to a final volume of 300 μl and incubated with 10 μl of medium slurry of streptavidin-coated magnetic beads (GE Healthcare) for 2 hours at room temperature under continuous rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The SPR analyses were carried out using the BIAcore 8K system with a streptavidin-coated (SA) chip (#BR-1005-30, GE Healthcare) at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... isoform 1 with N-terminal MRGS(H)8 and C-terminal Avi tag (Hismyosin VI (aa 992-1031)Avi) was immobilized on either MyOne T1 streptavidin-coated beads (Pierce) or Sera-Mag neutravidin-coated beads (GE Healthcare). The use of the type of beads was alternated during selection rounds ...
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bioRxiv - Molecular Biology 2023Quote: ... was immobilised to approximately 2100 response units (RU) on a flow cell of a Streptavidin coated (SA) sensor chip (GE healthcare) according to manufacturer’s directions while another flow cell was left blank for reference subtraction and monitoring any non-specific bindings ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Neuroscience 2023Quote: ... (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K, Na, or Li) was injected over the streptavidin-coated surface of a sensor chip SA (GE Healthcare). The amount of immobilized RNA was as follows ...
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bioRxiv - Biochemistry 2024Quote: ... or biotinylated CDO1 (bio-CDO1) was immobilized on a streptavidin chip using a Biacore T200 or a Biacore 8K (GE Healthcare). To determine the kinetics-affinity of the small molecules for proteins ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody conjugated with horseradish peroxidase was detected with ECL plus chemiluminescence kit (Amersham Biosciences/GE Healthcare, Piscataway NJ). The images were acquired with an HP Scanjet and densitometry determined using NIH ImageJ ...
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bioRxiv - Biochemistry 2022Quote: ... Bound secondary antibodies detected by chemiluminescence using the ECL kit according to the manufacturer’s instructions (GE Healthcare, Uppsala, Sweden). Finally ...
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bioRxiv - Immunology 2023Quote: ... Detection of HRP-linked antibodies was performed using chemiluminescence detection kits (ECL™ or ECL Plus™, GE Healthcare) according to the manufacturer’s instructions and visualized using ChemiDoc™ Touch Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide was diluted to 50 nM in running buffer (HBS-EP) and immobilized on a streptavidin sensor chip (sensor chip SA, GE Healthcare, USA). The analytes KEAP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with HRP-conjugated goat-anti-rabbit (GE Healthcare) and chemiluminescence imaging using LAS-4000 system (Fuji Film) ...
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bioRxiv - Microbiology 2022Quote: ... and goat anti-mouse or anti-rabbit HRP conjugates (GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... Cy3-labelled goat-anti-mouse mAb (PA43009, GE Healthcare, 1:3,000) was subsequently used as a secondary antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Genetics 2022Quote: ... and then incubated with goat-developed anti-rabbit (GE Healthcare, NA934) or goat-developed anti-mouse immunoglobulin G (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 000 dilution and HRP Goat anti-rabbit IgG (GE Healthcare, NA934) at 1:10,000 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... and HRP-labelled anti-mouse secondary antibody before bands being detected using an enhanced chemiluminescence kit (GE Healthcare, Buckingham, UK). β-actin (Sigma ...
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bioRxiv - Microbiology 2019Quote: ... secondary antibodies conjugated with horseradish peroxidase were used for detection by chemiluminescence using the Amersham ECL Prime Kit (GE Healthcare). Biochemical fractionation of infected cells was performed as previously described [19].
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or medium alone for 30 min at room temperature and added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... and further purified by affinity chromatography using resin attached with streptavidin or Ni-NTA and size-exclusion chromatography (SEC) using a Superdex 200 column (GE Healthcare Life Sciences) with the buffer containing 20 mM Tris pH 8.0 and 200 mM NaCl.
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of supernatant from each sample were mixed with 30 μl of pre-washed streptavidin-sepharose beads (GE Healthcare, 17-5113-01) and incubated for 3 hours at 4°C on a nutator ...
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bioRxiv - Neuroscience 2020Quote: ... antibody with respective secondary antibodies (1:5000, GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... goat polyclonal anti-GST (GE healthcare, 27-4577-01; 1:2000, IB); rabbit polyclonal anti-Cap1 [1 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and (HRP)-coupled goat anti-rabbit IgG (GE Healthcare, NA934VS; 1/10000), and developed with Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were detected with secondary antibodies conjugated to horseradish peroxidase (RPN4201 and RPN4301) and the ECL detection kit (both GE Healthcare). Visualization of protein bands was realized by LAS-4000 mini (Fujifilm) ...
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bioRxiv - Neuroscience 2020Quote: ... membranes were treated with peroxidase-conjugated secondary antibodies for 2h at room temperature and revealed using a chemiluminescence detection kit (ECL, GE Healthcare).