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Citations for GE Life Sciences :
201 - 238 of 238 citations for Streptavidin FITC Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: Experimental “affinity” between β-CD monomers and protein-conjugates were measured experimentally through surface plasmon resonance (SPR) with a Biacore X100 system (GE Healthcare Bio-Sciences, Pittsburgh, PA) according to previous protocols 31,32 ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were gently agitated at 4°C overnight prior to removal of unreacted FITC by size exclusion chromatography using PD-10 columns (GE Healthcare). Elution was performed using PBS ...
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bioRxiv - Immunology 2019Quote: ... washed with 1% BSA/PBS and incubated with Cy3-conjugated or FITC-conjugated goat anti-rabbit IgG (10 mg/ml; GE Healthcare) for 45 min respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was transferred to a tube containing 30 µl of pre-washed packed streptavidin beads (GE Healthcare, 17-5113-01). Streptavidin pulldown was done for 3 hours at 4°C ...
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bioRxiv - Biophysics 2019Quote: 500 resonance units (RU) of Biotinylated DCAF15-DDB1-DDA1 was immobilized to a streptavidin SA Sensor Chip (GE Healthcare Life Sciences) in a running buffer consisting of 50 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: Conversion beads were constituted of porous streptavidin-conjugated sepharose hydrogel with 34 μm average particle size (GE healthcare: GE17-5113-01) functionalized with biotinylated DNA constructs ...
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bioRxiv - Bioengineering 2022Quote: ... were immobilized on individual flow cells at 5 μl/min using a streptavidin-coated sensor chip (Series S Sensor chip SA, GE Healthcare). Three-fold serial dilutions of recombinant DLL4 proteins (N-EGF5 ...
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bioRxiv - Neuroscience 2021Quote: ... to a final volume of 300 μl and incubated with 10 μl of medium slurry of streptavidin-coated magnetic beads (GE Healthcare) for 2 hours at room temperature under continuous rotation ...
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bioRxiv - Plant Biology 2019Quote: ... Slides were rinsed as before and incubated with a 1/100 dilution (in PBS/BSA) of biotinylated/streptavidin/horseradish peroxidase complex (GE Healthcare) before a final PBS/BSA rinse ...
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bioRxiv - Microbiology 2021Quote: ... between 400 to 600 RU of biotinylated anti-IFX monoclonal antibodies were immobilised on a streptavidin-coated sensor chip prepared using the Biotin CAPture kit (GE Healthcare); a biotinylated mouse monoclonal antibody (OX68 ...
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bioRxiv - Physiology 2020Quote: ... and incubated lysate equivalent to 2 mg of protein with 50 μL Streptavidin Sepharose High-Performance beads (GE Healthcare, IL, USA) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: The SPR analyses were carried out using the BIAcore 8K system with a streptavidin-coated (SA) chip (#BR-1005-30, GE Healthcare) at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2% Triton X-100) and incubated 1 hour at RT or 16 hours at 4°C with HRP-coupled streptavidin (RPN1231VS, GE Healthcare) diluted 1:20000 in BSA blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... isoform 1 with N-terminal MRGS(H)8 and C-terminal Avi tag (Hismyosin VI (aa 992-1031)Avi) was immobilized on either MyOne T1 streptavidin-coated beads (Pierce) or Sera-Mag neutravidin-coated beads (GE Healthcare). The use of the type of beads was alternated during selection rounds ...
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bioRxiv - Molecular Biology 2023Quote: ... was immobilised to approximately 2100 response units (RU) on a flow cell of a Streptavidin coated (SA) sensor chip (GE healthcare) according to manufacturer’s directions while another flow cell was left blank for reference subtraction and monitoring any non-specific bindings ...
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Neuroscience 2023Quote: ... (G4C2)4 RNAs biotinylated at the 5′ end in 10 mM HEPES pH 6.8 and 500 mM MCl (M = K, Na, or Li) was injected over the streptavidin-coated surface of a sensor chip SA (GE Healthcare). The amount of immobilized RNA was as follows ...
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bioRxiv - Biochemistry 2024Quote: ... or biotinylated CDO1 (bio-CDO1) was immobilized on a streptavidin chip using a Biacore T200 or a Biacore 8K (GE Healthcare). To determine the kinetics-affinity of the small molecules for proteins ...
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bioRxiv - Immunology 2023Quote: ... The S proteins were then separated by chromatography using resin attached with streptavidin and further purified by size exclusion chromatography using a Superose 6 10/300(GE Healthcare) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was rotated for 1 h at 4 °C in presence of 50 µL of streptavidin sepharose (50% slurry, GE Healthcare). Beads were washed three times with NET buffer ...
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bioRxiv - Bioengineering 2021Quote: ... These cells were then visualised using FITC (495/519 nm) and DAPI (358/451 nm) channel using IN Cell Analyzer 6000 (GE Life Sciences) at 40x magnification.
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bioRxiv - Microbiology 2022Quote: ... The FITC-TB_KKG6K bound to specific phosphoinositides was detected fluorometrically with a Typhoon FLA 9500 biomolecular imager (GE Healthcare, Chicago, IL, USA) equipped with a 473 nm laser and filters for excitation/emission wavelengths of 494/520 nm ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified lysate obtained after centrifugation (30 min at 20000 × g, 4°C) was first run on a streptavidin affinity column (StrepTrap® HP, GE Healthcare) equilibrated with septin 1 buffer (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cell Biology 2022Quote: ... The peptide was diluted to 50 nM in running buffer (HBS-EP) and immobilized on a streptavidin sensor chip (sensor chip SA, GE Healthcare, USA). The analytes KEAP1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then lysed in lysis buffer and equal amounts of their proteins were subjected to Streptavidin beads-based affinity isolation (GE Healthcare, USA). After 30 minutes incubation at 4 degrees ...
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bioRxiv - Biochemistry 2020Quote: ... cleared lysates from ARL15A86L expressing Flp-In™ T-REx™ HeLa cells were incubated with streptavidin beads (17-5113-01, 5 ml, GE Healthcare). Trypsin digestion on beads was performed and peptides were collected in water ...
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bioRxiv - Synthetic Biology 2022Quote: CM3 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or medium alone for 30 min at room temperature and added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: CM5 sensor chips were coupled with streptavidin to near saturation (typically between 4000 and 7000 RU) using the amine coupling kit (GE Healthcare Life Sciences). After streptavidin was coupled ...
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bioRxiv - Microbiology 2022Quote: ... and further purified by affinity chromatography using resin attached with streptavidin or Ni-NTA and size-exclusion chromatography (SEC) using a Superdex 200 column (GE Healthcare Life Sciences) with the buffer containing 20 mM Tris pH 8.0 and 200 mM NaCl.
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bioRxiv - Molecular Biology 2023Quote: ... nitrocellulose membranes were blocked in 3 % BSA in TBST and stained with HRP-conjugated streptavidin (GE Healthcare Life Science, Cat# RPN1231vs,1:2500) in blocking solution ...
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bioRxiv - Cell Biology 2023Quote: ... equal amounts of supernatant from each sample were mixed with 30 μl of pre-washed streptavidin-sepharose beads (GE Healthcare, 17-5113-01) and incubated for 3 hours at 4°C on a nutator ...
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bioRxiv - Cancer Biology 2021Quote: ... Cellular lysates were cleared by centrifugation and 300 μg of total protein were added to 30 μL of pre-equilibrated streptavidin-sepharose matrix (GE Healthcare, Chicago, IL, USA). Following 1 h at 4 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... following the manufacturer’s protocol using 15 µl of PCR product and 2 µl Streptavidin Sepharose High Performance beads (GE Healthcare, cat. GE17-5113-01).
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bioRxiv - Systems Biology 2021Quote: ... Samples were centrifuged at 16,000 x g for 20 minutes and the supernatant used for affinity purification of biotinylated proteins using 25 µl of washed streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301) for 16 hours with rotation at 4℃ ...
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bioRxiv - Systems Biology 2020Quote: ... Samples were centrifuged at 15,000 × g for 15 minutes and the supernatant was used for biotinylated protein capture using 15 μl of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After 6 hours ...
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bioRxiv - Molecular Biology 2023Quote: Fetal bovine serum and cosmic calf serum were mixed in a 1:1 ratio and incubated with pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). Briefly ...