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Citations for GE Life Sciences :
1 - 50 of 1648 citations for SpectraDye Antibody Labeling Kit 650 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... followed by [α-32P]dCTP-labeling by random priming (Multiprime DNA labeling kit; GE Healthcare).
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bioRxiv - Microbiology 2021Quote: ... The probe was produced with a Rediprime II labeling kit (GE Healthcare). Hybridization was performed as described by Dienst et al ...
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bioRxiv - Cell Biology 2023Quote: ... Labeling was performed using an enhanced chemiluminescence (ECL) select kit (GE Healthcare Bio-Sciences). The chemiluminescence signals were detected in an ImageQuant LAS4000 mini (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2020Quote: ... Probe labeling for detection was performed using AlkPhos Direct Labeling and Detection System (GE Healthcare) according to the manufacturer’s description ...
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bioRxiv - Plant Biology 2020Quote: ... α [32P] dCTP-labeled probes were generated by random priming (Megaprime DNA labeling kit; GE Healthcare). Hybridization was performed in Church Buffer (1% [w/v] BSA ...
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bioRxiv - Cell Biology 2020Quote: ... following labeling with Cy3 dye (GE Healthcare). Following incubation in the dark at 27°C for 20 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling with Cy5-NHS ester (GE Healthcare, PA15101) yielded 54–70% labeling efficiency ...
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bioRxiv - Plant Biology 2021Quote: ... Amersham Alkphos Direct labeling reagents (GE Healthcare, Buckinghamshire, UK) were used for probe labeling and subsequent hybridization ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction was quenched with ∼650 μM β-mercaptoethanol and desalted using PD-10 columns (GE healthcare). The extent of reaction was checked using 1D intact weight ESI mass spectrometry (SIRCAMs ...
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bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% dextran sulfate) at 65°C probed with 32P-labeled CLB3 DNA probes prepared via Amersham Megaprime DNA labeling kit (GE Healthcare) and Illustra ProbeQuant columns (GE Healthcare) ...
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bioRxiv - Microbiology 2019Quote: ... PCR fragments were then labelled using Megaprime DNA Labeling Systems (GE Healthcare). Primers used are listed in Table 1.
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bioRxiv - Microbiology 2020Quote: ... using the Amersham ECL Direct Labeling and Detection System® (GE Healthcare). Fungal RNA was isolated using TRIzol reagent (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare). Immobilization levels ranged from 7,800-9,000 response units (RU ...
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bioRxiv - Biochemistry 2019Quote: ... The excess labeling agent was eliminated by using Hitarp desalting column (GE Healthcare). The labeled peptides were further purified and separated from the unlabeled peptides by RP-HPLC using a Jupiter 300 Å C5 column (Phenomenex) ...
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bioRxiv - Biochemistry 2020Quote: ... Confirmation of protein labeling was verified using a Typhoon imaging system (GE Healthcare). The labeled protein was aliquoted ...
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bioRxiv - Bioengineering 2024Quote: ... Radioactive probes were prepared by random-primed 32P labeling (GE Healthcare, Chicago, IL) of gel-purified DNA fragments of the following genes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Probes were labled with 32P-dCTP (Hartmann Analytic, Braunschweig) by the use of the Rediprime II DNA Labeling Kit (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... in which aliquots (68 μL) are automatically withdrawn from the large reaction vessel (650 μL) and loaded into a Resource Q column (GE Healthcare) without the need for prior quenching of the reaction ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were then resuspended in 650 µL of dialysis buffer and 100 U of Thrombin (GE Healthcare, #GE27-0846-01), to a final volume of 1 mL ...
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bioRxiv - Plant Biology 2020Quote: ... radioactively labelled with α32P[CTP] using the Megaprime DNA Labeling System (GE Healthcare Life Sciences), and hybridized at 65 °C.
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bioRxiv - Cell Biology 2020Quote: Polyclonal anti-GST antibodies (GST Capture Kit, GE Healthcare) were amine coupled on to a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were detected using the ECL detection kit (GE Healthcare). Prior to re-probing with different antibodies ...
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bioRxiv - Immunology 2020Quote: ... Antibodies were detected using the ECL detection kit (GE Healthcare). Prior to re-probing with different antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Amersham Rediprime II Random Prime Labeling System (RPN 1633; GE Healthcare, Chalfont St Giles, UK). Membranes were hybridized (250 mM Na2HPO4 ...
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bioRxiv - Microbiology 2022Quote: Southern blots were accomplished following the specifications of ECL Direct Nucleic Acid Labeling and Detection System (GE Healthcare, UK). Briefly ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-reactive bands were detected with chemiluminescence substrate ECL kit (GE Healthcare) using ChemiDoc™ MP Systems (Bio-Rad ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Molecular Biology 2023Quote: ... Labeling of Spt7 and residual dye depletion were confirmed by in-gel fluorescence imaging on a Typhoon imager (GE Healthcare). In vitro transcription activity of the nuclear extract was verified by the in vitro transcription assay described below.
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bioRxiv - Neuroscience 2021Quote: ... HRP-conjugated secondary antibodies signal was revealed using ECL Prime kit (GE Healthcare) and captured using the ChemiDoc imager (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Horseradish peroxidase conjugated secondary antibodies and enhanced chemiluminescence reagents (ECL kit) (GE Healthcare) were used for detection ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Labeled proteins were separated from the free fluorophore using a NAP-5 Sephadex G-25 desalting columns in labeling buffer (GE Healthcare). The labeling efficiency was determined using an extinction coefficient of 5400 M-1cm-1 at 335 nm for IAEDANS and the protein concentration was determined by Bradford assay using BSA as a standard ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescently labeling of Pol β followed the same procedure using an Amersham Cy5 mono-reactive NHS ester dye pack (GE healthcare). Labeling efficiency was determined by calculating the molar ratio of dye-to-protein within the solution by measuring absorbance at 280 nm and 530 nm for APE1-Cy3 or 649 nm for Pol β-Cy5 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 32P-labeled DNA probes containing gag sequences were made by randomly primed DNA synthesis with Amersham Megaprime DNA labeling System (GE Healthcare). gag sequences were amplified by PCR from plasmids pGTy1-H3 (Genbank ...
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bioRxiv - Neuroscience 2020Quote: ... Northern blot probes to detect different isoforms of homothorax were prepared by Amersham Megaprime DNA Labeling System (GE Healthcare Life Sciences), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... A radioactively labeled probe corresponding to the coding region of TbGPI2 was generated using a Megaprime DNA-labeling system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... horseradish peroxidase-conjugated secondary antibodies and an ECL kit (GE Healthcare/Amersham Pharmacia Biotech) were used to detect protein signals ...
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bioRxiv - Microbiology 2020Quote: ... The modified Cy3 labeled fragment was generated by labeling an amine-modified RNA containing a 5′ monophosphate ordered from Horizon Discovery with monoreactive Cy3 (GE Life Sciences) and the labeled RNA was purified by reverse-phase HPLC ...
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bioRxiv - Cancer Biology 2020Quote: DNA probes complementary to the TP53 coding region or the 3′UTR were labeled with dCTP [α-32P] using the Amersham Megaprime DNA labeling system (GE Healthcare). Primers used for probe synthesis from human cDNA are listed in Supplementary Table 1 ...
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bioRxiv - Biochemistry 2019Quote: ... Labeling was confirmed by SDS-PAGE and then scanning the gel for fluorescence using a Typhoon FLA 9500 (GE Healthcare Life Sciences). Degree of labeling (DOL ...
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bioRxiv - Evolutionary Biology 2019Quote: ... gene specific fragments that were used for VIGS (primer pairs listed in Table S1) were radiolabeled with [α-32P] dCTP (Perkin-Elmer) using the Rediprime II DNA Labeling System (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was transferred from the purification buffer into the labeling buffer (Na-PO4 20 mM, pH 8.3) supplemented with OG (30 mM) using PD-10 columns (GE Healthcare, Solingen, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and probed with 5’ end-labeled oligonucleotides or PCR fragments labeled by random priming (Megaprime DNA Labeling System, GE Healthcare Life Sciences). Hybridizations were performed in UltraHyb or UltraHyb-Oligo (Ambion ...
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Neuroscience 2019Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Neuroscience 2020Quote: ... The titering method was for encapsidated vector genomes per ml by a slot-blot method against a standard curve using the Amersham ECL Direct Nucleic Acid Labeling and Detection Systems (GE Healthcare Bio-Sciences).
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bioRxiv - Genetics 2022Quote: ... Northern blots were hybridized with DNA probes generated by a random-primed labeling reaction with use of Amersham Ready-To-Go DNA Labelling Beads (GE Healthcare Life Sciences) supplemented by [α-32P]dCTP and a corresponding PCR fragment as a template ...
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bioRxiv - Genetics 2023Quote: ... Northern blots were hybridized with DNA probes generated by a random-primed labeling reaction with use of Amersham Ready-To-Go DNA Labelling Beads (GE Healthcare Life Sciences) supplemented with [α-32P]dCTP and a corresponding PCR fragment as a template ...