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Citations for GE Life Sciences :
151 - 200 of 222 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed three times with 20x bead volume of TBS using a magnetic tube rack (MagRack 6, GE Life Sciences).
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bioRxiv - Biophysics 2021Quote: ... the protein was further diluted by adding an equal volume of Q1 and loaded onto a HiTrap Q HP anion exchange chromatography column (GE Healthcare) equilibrated in buffer Q1 ...
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bioRxiv - Immunology 2020Quote: ... cells were fed with 5% of the starting volume with Cell Boost7a and 0.5% Cell Boost7b (Hyclone GE Healthcare Life Sciences). Cell counts and glucose was measured as previously described,70 On day 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl) of lgG Sepharose beads (GE Healthcare) for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... were added to the lysate (2 μg/ml cyclin B1 and 10 μg/ml cyclin A final concentration) together with protein G Sepharose (20 μl of 50% slurry bead volume; GE Healthcare) for 16 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein eluted with a gradient from 20 to 500 mM imidazole over 10 column volumes using an AKTA Pure (GE Healthcare).
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bioRxiv - Bioengineering 2022Quote: The shift in elution volume of the antigens was determined by size exclusion chromatography (SEC) on a Superdex 200 GL column (GE HealthCare) equilibrated with 50 mM Tris (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... Peak fractions were diluted with 2 volumes of buffer H and loaded onto a 1-mL SP HP column (GE Healthcare) and washed with buffer C (50 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The protein was then concentrated to a final volume of 500 μl using Vivaspin 20 with a molecular weight cut off (MWCO) of 5 kDa (GE Healthcare). The sample was loaded on a Superose6 Increase 10/300 GL size exclusion column from GE Healthcare that had been equilibrated with size exclusion buffer (for INpro-28R ...
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bioRxiv - Immunology 2020Quote: Blood from patients with melanoma was collected in heparinized or EDTA tubes and was diluted with an equal volume of PBS before being layered over a Ficoll Paque PLUS gradient (GE Healthcare) in 50 mL conical tubes that were centrifuged at room temperature for 15 minutes at 932 g ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained structural proton images and DTI for assessment of volumes and diffusivity using a flexible 16-channel coil (GE Healthcare). Anatomy and volumes were evaluated using T1-weighted (TR/TE = 14/500 ms ...
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bioRxiv - Neuroscience 2021Quote: ... to a final volume of 300 μl and incubated with 10 μl of medium slurry of streptavidin-coated magnetic beads (GE Healthcare) for 2 hours at room temperature under continuous rotation ...
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bioRxiv - Biophysics 2019Quote: ... All fractions identified to contain the cleaved protein were concentrated to a volume of 5 mL and loaded onto a HiLoad 16/60 Superdex 75 gel filtration column (GE Healthcare) that had been pre-equilibrated with either crystallography buffer (20 mM Bis-Tris pH 7.0 ...
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bioRxiv - Biophysics 2020Quote: ... Bound proteins were eluted using a linear gradient (10 column volumes) from 0 to 1 M NaCl in buffer A using an Äkta Purifier (GE Healthcare). Elution fractions (1.6 ml ...
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Bridging of nucleosome-proximal DNA double-strand breaks by PARP2 enhances its interaction with HPF1bioRxiv - Biochemistry 2019Quote: ... concentrated using centrifugal filters to bring down the volume to ∼1 mL and injected onto a Superdex 200 16/60 size exclusion column (GE Healthcare) previously equilibrated in HEPES 50 mM ...
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bioRxiv - Microbiology 2020Quote: ... to a volume of 2 ml and applied to size exclusion chromatography (SEC; HiLoad 26/600 Superdex 200 pg, GE Healthcare), After SEC ...
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bioRxiv - Biochemistry 2021Quote: ... with the addition of 1 mg of 3C protease per 3 L of culture volume and further separated on a Superdex 200 HiLoad column (GE Healthcare) in 50mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... AtGDH sample was concentrated and further purified with the Superdex 200 pg 16/600 gel filtration column (120 ml bed volume, GE Healthcare) pre-equilibrated with buffer A ...
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bioRxiv - Microbiology 2020Quote: ... The molecular weight of AmvR in solution was estimated by comparing its elution volume to those of a set of molecular weight standards (HMW and LMW calibration sets, GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... and the HE ectodomain in the supernatant was concentrated to a volume of ∼150 µl and injected on a Superdex200 increase column (GE Healthcare) pre-equilibrated in 10 mM Tris pH 8 and 50 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300, bead volume: 24 ml; GE Healthcare), also equilibrated with WB ...
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bioRxiv - Microbiology 2020Quote: ... The quality of the amphipol associated complex was controlled by performing an additional size exclusion step on a Superdex 200 increase column (10/300, bead volume: 24 ml; GE Healthcare).
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bioRxiv - Immunology 2021Quote: The polyclonal IgGs from serum or plasma were purified by incubating with equal volume of Protein A and G sepharose resin (GE Healthcare) at 4°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was filtered on 0.45 µm units before loading onto a nickel-Sepharose Fast Flow column (1mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The PLP protein was eluted at 160 mM imidazole ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... equal volumes (125 μl containing 6250 µg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
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bioRxiv - Plant Biology 2022Quote: ... and the supernatant was clarified using 0.45 µm filters before loading onto a nickel-Sepharose Fast Flow column (5 mL of bed volume) using an ÄKTATM Start Fast Protein Liquid Chromatography system (GE Healthcare). The column was washed with 40 mL of extraction buffer ...
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bioRxiv - Microbiology 2023Quote: ... The clarified lysate was incubated (overnight, at 4 °C, with gentle agitation) with 1 ml bed volume of GSH-Sepharose (GE Healthcare). Next ...
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bioRxiv - Cancer Biology 2023Quote: ... equal volumes (125 μl containing 6250 μg) of two different kind of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
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bioRxiv - Genomics 2023Quote: ... The phage DNA is then purified using 1.6x volume of homebrewed solid phase reversible immobilization (SPRI beads) (1mL Sera-Mag SpeedBeads (GE Healthcare, GE1715210401150), 9g PEG 8000 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... equal volumes (125 μl containing 6250 µg) of two different kinds of paramagnetic carboxylate modified particles (SpeedBeads 45152105050250 and 65152105050250; GE Healthcare) were mixed ...
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bioRxiv - Immunology 2024Quote: IgGs were purified from ∼2 mL of plasma with an equal volume of Protein A Sepharose resin (GE Healthcare Life Sciences) and incubated with the resin for three days at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The eluate was concentrated to a volume of 5 mL and loaded onto a size-exclusion-chromatography column (Superdex 16/60, GE Healthcare); HR3 LBD was eluted in a buffer of 150 mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to ∼2 µM for cryo-EM sample preparation.
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The splicing product was concentrated to a volume of ∼500 µL and loaded onto a Superose 75 10/300 GL size-exclusion column (GE Healthcare) which is pre-equilibrated with complex buffer (20mM HEPES,150mM NaCl ...
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bioRxiv - Biochemistry 2024Quote: ... Then the complex was concentrated to a volume of ∼100 µL and loaded onto a Superose 6 5/150 GL size-exclusion column (GE Healthcare). Peak fractions were pooled and concentrated to 2µM for cryo-EM sample preparation.
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bioRxiv - Plant Biology 2019Quote: We extracted RNA according to Oliveira et al.33 and evaluated its integrity and purity using agarose gel electrophoresis and a NanoVue spectrophotometer (GE Healthcare Life Sciences). lllumina TruSeq RNA Sample Preparation kits (Illumina ...
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bioRxiv - Immunology 2020Quote: ... The rest of the blood were diluted with PBS (pH7.2) to 2X of original volume and layered over 15 ml Ficoll (GE Healthcare, Waukesha, WI) in SepMate-50 tubes (Vancouver ...
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bioRxiv - Neuroscience 2021Quote: ... a preparative IEC chromatography was carried out on AKTA Purifier onto the anion exchanger resin Source 15Q (GE HealthCare, 20 ml volume). The column was equilibrated at room temperature (∼25 °C) ...
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bioRxiv - Cell Biology 2021Quote: ... A volume of 2.5 ml of soluble fraction was incubated with 100 μl of Glutathione Sepharose beads (GE Healthcare, 17-0756-01) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was transferred to a new 50 ml falcon tube and 250 µl bed volume of Ni-Sepharose (GE Healthcare Australia) was added to the lysate ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant was transferred to a new 50 ml falcon tube and 250 µl bed volume of PBS-washed glutathione Sepharose beads (GE Healthcare Australia) was added to the lysate ...
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bioRxiv - Immunology 2022Quote: ... at a density of 0.3 x 106 cells/mL in 10 mL total volume/plate of complete culture medium (RPMI 1640 plus 10% heat-inactivated fetal bovine serum (FBS, GE Healthcare HyClone), 50 μM 2-mercaptoethanol ...
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bioRxiv - Plant Biology 2019Quote: ... An appropriate volume was applied to a 1 ml loop of a gel filtration column (Superdex75 10/300 GL, GE Healthcare, www.gehealthcare.com) of an Äkta Avant System (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... was incubated with or without 200 µM dGTP in a total volume of 50 µl for 5 min prior to size exclusion chromatography (SEC) using an Ettan™ LC System (GE Healthcare). Samples were injected into an analytical Superdex 200PC3.2 column (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were then captured using 15 µl (packed volume) of pre-washed Streptavidin agarose beads (GE Healthcare Life Science, Cat# 17511301). After overnight incubation at 4 °C with rotation ...
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bioRxiv - Cell Biology 2019Quote: ... The RNA pellets were suspended in DEPC water and the samples were quantified at 260/280 nm in a spectrophotometer (NanoVue – GE Life Sciences, Buckinghamshire, England).
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bioRxiv - Physiology 2019Quote: The optical density of cell suspensions was measured at a wavelength of 600 nm using an Ultrospec 10 spectrophotometer (GE Healthcare, Chicago, IL, USA). The cell dry weight (CDW ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 4000 rpm at 4°C for 20 minutes in order to determine the total protein concentration through NanoVue spectrophotometer (GE Healthcare, Chicago, IL, USA). A total of 40 µg protein was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...