Labshake search
Citations for GE Life Sciences :
501 - 550 of 2934 citations for Sialic Acid Binding Ig Like Lectin 5 SIGLEC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... and loaded onto a 5 mL HiTrap SPHP column (GE Healthcare). A linear 5 CV gradient ...
-
bioRxiv - Biochemistry 2023Quote: ... and subsequently applied to a 5-mL HisTrap column (GE Healthcare) pre-equilibrated with wash buffer (25 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... and subsequently injected onto a 5-mL HisTrap column (GE Healthcare) connected to an ÄKTA FPLC system ...
-
bioRxiv - Molecular Biology 2023Quote: ... The sample was injected in S200 5/150 column (GE Healthcare) pre-equilibrated with KMNZ reconstruction buffer and the peak fraction was taken for structural analysis.
-
bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL HisTrap FF column (GE Healthcare) at a rate of 2 mL/min ...
-
bioRxiv - Biophysics 2023Quote: ... 5 ml HisTrap FF crude columns were bought from GE Healthcare. NuPAGE® MES SDS Running Buffer (20X) ...
-
bioRxiv - Biochemistry 2023Quote: ... RNGTT was further loaded onto Mono Q (5/50GL, GE Healthcare) while CMTR1 was further loaded onto a Heparin column (5/50GL ...
-
bioRxiv - Microbiology 2023Quote: ... and applied to 5 ml HiTrap Q HP column (GE Healthcare) equilibrated with TGE buffer containing 50 mM NaCl and 1 mM β-mercaptoethanol ...
-
bioRxiv - Cell Biology 2022Quote: ... before loading onto a 5 ml HisTrap HP column (GE Healthcare). The protein was eluted in 300 mM imidazole ...
-
bioRxiv - Synthetic Biology 2023Quote: ... using a 5 mL HisTrap FF column (GE Healthcare Life Sciences) as previously described3 except that an optimized buffer gradient was employed consisting of a first wash step of 5 column volumes (CV ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatant was applied to HisTrap HP 5 ml column (GE Healthcare) pre-equilibrated in 25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Bioengineering 2023Quote: ... with Nickel affinity chromatography (5 mL HisTrap HP column, GE Healthcare), anion ion exchange chromatography (5 mL HiTrap Q FF column ...
-
bioRxiv - Bioengineering 2023Quote: ... Affinity chromatography utilizing a HisTrap HP 5 mL column (GE Healthcare) was then employed to purify the protein in binding buffer consisting of 50 mM NaPO4 (pH 7.4) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 mL Ni-NTA HisTrap columns (GE Healthcare, Chicago, IL, USA) were used ...
-
bioRxiv - Bioengineering 2024Quote: ... and loaded onto a HisTrap HP 5 ml column (GE Healthcare) at 4 °C using an ÄKTA Pure 25 M (GE Healthcare) ...
-
bioRxiv - Biochemistry 2024Quote: ... proteins were buffered in PBS using NAP-5 columns (GE healthcare) or Zeba spin columns (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (GE Healthcare; # NA934V) incubated in PBST for 1h at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... Peroxidase-labelled anti-mouse IgG antibody and anti-rabbit IgG antibody (GE Healthcare, Chicago, IL, USA) were used as secondary antibodies for immunoblotting ...
-
bioRxiv - Microbiology 2020Quote: ... Antibodies were detected with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare, Chicago, Il USA). Membranes were rinsed in PBS + 0.05% Tween-20 and visualized by ECL in an ImageQuant LAS 4000mini (GE Healthcare ...
-
bioRxiv - Cancer Biology 2023Quote: ... Secondary horseradish peroxidase-conjugated antibodies (anti-mouse/rabbit IgG HRP-linked whole antibody donkey; GE Healthcare) were used ...
-
bioRxiv - Cell Biology 2023Quote: ... Primary antibodies were detected by an HRP-conjugated donkey anti-rabbit secondary antibody (#NA934V; GE Healthcare) or HRP-conjugated donkey anti-mouse secondary antibody (#NXA931 ...
-
bioRxiv - Neuroscience 2020Quote: The bacterial expression construct GST-MPP2-SH3-GK was generated by cloning the fragment encoding amino acids 220-552 (SH3GK) of mouse MPP2 into pGEX-6P-1 (GE Healthcare). GST-PSD-95-SH3GK was generated as described before [25] ...
-
Ouabain enhances cell-cell adhesion mediated by β1-subunits of the Na+,K+-ATPase in CHO fibroblasts.bioRxiv - Cell Biology 2019Quote: Total extract of CHO cells expressing the β1His6 construct were immobilized with nickel-nitrilotriacetic acid beads (Ni+-NTA, His Trap FF column; GE Healthcare) equilibrated with 10 ml of RIPA buffer containing protease inhibitors ...
-
bioRxiv - Biochemistry 2020Quote: ... the supernatants were collected for a three-step purification by nickel-nitrilotriacetic acid affinity chromatography (HisTrap FF column, GE Healthcare, Inc.), ion exchange chromatography (HiTrap Q HP column ...
-
bioRxiv - Cell Biology 2019Quote: ... phenolics and nucleic acids in extracted protein were removed using the 2-D Clean-Up kit (GE Healthcare, Piscataway, NJ, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... All recombinant proteins were purified using Ni2+-nitrilotriacetic acid agarose (Ni-NTA) column followed by size-exclusion chromatography (Superdex 200 column from GE Healthcare) in a final buffer containing 50 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Radiolabeled nucleic acids were resolved on 19% denaturing polyacrylamide-urea gel and visualized on a Typhoon FLA 9500 imager (GE healthcare).
-
bioRxiv - Neuroscience 2021Quote: ... Nucleic acids bound non-specifically to the protein constructs were removed by performing heparin affinity chromatography (HiTrap Heparin HP; GE Healthcare), while being connected to an Äkta pure system ...
-
bioRxiv - Microbiology 2021Quote: ... the pure ParD2-ParE2 complex in SEC buffer was loaded onto a nickel–nitrilotriacetic acid affinity column (connected to an AKTAexplorer FPLC system, GE Healthcare) previously equilibrated in the same SEC buffer ...
-
bioRxiv - Genomics 2021Quote: ... and AsNRDE-3 amino acids 45-246 (HQ611973) were fused in frame with GST using the Bam H1 site of pGEX-6P-1 (GE Healthcare), the proteins were expressed in E ...
-
bioRxiv - Biochemistry 2022Quote: ... The KlBre1 RBD-Rad6 complex was purified by nickel–nitrilotriacetic acid agarose (Ni-NTA, Smart Life sciences) and ion exchange (Hitrap Q HP, GE healthcare) columns ...
-
bioRxiv - Microbiology 2023Quote: ... Southern blotting to confirm the genotypes were performed using ECL™ Direct Nucleic Acid Labeling and Detection kit (GE Healthcare, USA). Probe (650 bp ...
-
bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP-linked antibody (NA934; GE Healthcare) as secondary antibody.
-
bioRxiv - Cell Biology 2019Quote: ... HRP-linked whole antibody (GE Healthcare) and polyclonal rabbit anti-goat (Dako) ...
-
bioRxiv - Molecular Biology 2019Quote: ... HRP conjugated second antibodies (GE Healthcare) were used at a concentration of 1:5000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse secondary antibody (GE Healthcare) was used at 1:10,000 dilution and anti-rabbit secondary antibody (Cell Signaling Technology ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were diluted 1:10,000 in TBS + 0.1% Tween-20 and applied to the membrane for 1 hr ...
-
bioRxiv - Microbiology 2020Quote: ... IgG1 or IgG2a antibodies (GE Healthcare). The plates were washed and developed with 50 μl of TMB substrate per well until blue color appeared ...
-
bioRxiv - Cell Biology 2019Quote: ... Species-appropriate secondary antibodies (GE Healthcare) and ECL Prime Western Blotting Detection reagent (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... Secondary antibody (GE Healthcare, Buckinghamshire, UK) was used in a 1:2000 titer in 5% milk/Tris-buffered saline–Tween 20 for 1 hour at room temperature with agitation ...
-
bioRxiv - Synthetic Biology 2022Quote: ... or anti-rabbit antibody (GE Healthcare) as secondary antibodies ...
-
bioRxiv - Plant Biology 2023Quote: ... HRP-conjugated secondary antibodies (GE Healthcare) were used ...
-
bioRxiv - Cell Biology 2020Quote: ... HRP-conjugated anti-rabbit IgG donkey antibody and HRP-conjugated anti-goat IgG donkey antibody (GE Healthcare), HRP-conjugated Protein-G (abcam) ...
-
bioRxiv - Cell Biology 2020Quote: ... or anti-CSP repeat antibody – mAB 3D11 (Yoshida et al., 1980) and HRP bound antibodies (GE healthcare, Thermo Fischer Scientific ...
-
bioRxiv - Genomics 2020Quote: ... The MyoD-FL protein in flow through was diluted with pre-cooling 20 mM Tris-HCl pH 8.0 to ensure a low NaCl concentration to remove excess nucleic acids by HiTrap SP FF column (GE Healthcare, Beijing, China) with buffer A containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Plant Biology 2019Quote: ... containing 0.3 mmol L-1 NH4HCOO (adjusted to pH 3.5 with formic acid) delivered by a Pharmacia 2248 HPLC pump (GE Healthcare, Munich, Germany) were added just after the column via a mixing tee valve ...
-
bioRxiv - Biochemistry 2019Quote: The RRM domain of RBFOX2 (amino acids 100-194) was cloned into the pGEX6P-1 expression vector (GE Healthcare, 28-9546-48) downstream of a GST-SBP tandem affinity tag ...
-
bioRxiv - Plant Biology 2020Quote: ... coli codon optimized bZip domain of protein Cre06.g310500.t1.2 (amino acid position 141-203) was cloned into the GST-containing vector pGEX-4T-2 (GE Healthcare Life Sciences) by the company GeneScript ...
-
bioRxiv - Biochemistry 2021Quote: ... the culture filtrate was applied to a 20-mL nickel–nitrilotriacetic acid–sepharose column connected to an ÄKTA express FPLC system (GE Healthcare Lifesciences). After washing with 10 column volumes of working buffer containing 20 mM imidazole ...