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Citations for GE Life Sciences :
1 - 50 of 2537 citations for Semicarbazide Hydrochloride Sem 13C 99%; 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT). MCF7 cells used were authenticated to be 100% match to MCF7 cells (ATCC ...
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bioRxiv - Cancer Biology 2021Quote: ... and 100 units/ml penicillin-100 μg/ml streptomycin (GE Healthcare, Logan UT) at 37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 U/ml penicillin and 100 mg/ml streptomycin (HyClone; GE Healthcare Life Sciences). All cells were routinely cultured in a humidity incubator at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and ethanolamine hydrochloride (EA) were obtained from GE Healthcare/Cytiva (Marlborough ...
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bioRxiv - Molecular Biology 2023Quote: ... and ethanolamine hydrochloride (EA) were obtained from GE Healthcare/Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 U/mL penicillin/0.1 mg/mL streptomycin (GE Healthcare, USA), 1% L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... and transferred onto a methanol-activated PVDF membrane (GE Healthcare) at 60 V for 12 h at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... before being blotted onto methanol-activated 0.45μm PVDF membranes (GE Healthcare) at 300mA for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... 100 U/mL of Penicillin (GE Healthcare, catalog SV30010), and 100 µg/mL of Streptomycin (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... and 100 U/mL HyClone penicillin-streptomycin (GE Healthcare) at 37°C and 5% CO2.
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bioRxiv - Immunology 2019Quote: ... and 100 µg/mL of Streptomycin (GE Healthcare, catalog SV30010)) at 37 °C/5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... and 100 mg/mL streptomycin (HyClone, GE Healthcare Life Sciences), at 37 °C and 5% CO2 ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.5 ml of 100 kU DNase I (GE healthcare) for 10 minutes at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... For StrepII IP 25 μl Streptactin Sepharose® High Performance beads (GE Healthcare, GE28-9355-99) and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cell suspension volume was adjusted to 33.4 mL with PBS and 10 mL of 100% isotonic Percoll (9 mL Percoll PLUS (GE Healthcare), 1 mL 10x PBS without Ca and Mg ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a methanol-activated Amersham Hybond PVDF membrane (GE Healthcare) at 100 V for 1 h at room temperature using Mini Trans-Blot Cell system (Biorad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Patients were injected with 100–120 mL iohexol (Omnipaque, GE Healthcare) at an injection rate of 4–5 mL/s ...
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bioRxiv - Neuroscience 2021Quote: ... The chip was deactivated by 1 M Ethanolamine hydrochloride-NaOH (GE Healthcare Life Sciences) at a flow rate of 10 μL/min for 420 s ...
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bioRxiv - Cancer Biology 2022Quote: ... mixed with 1.2 ml of fetal bovine serum (FBS) and 3.6 ml of 100% Percoll (GE Healthcare). The Percoll cell suspension was overlaid with 1 ml of 10% FBS in DMEM and spun at 800g for 15 min ...
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bioRxiv - Immunology 2020Quote: ... and penicillin (100 U/mL) (RPMI++) were transduced with filtered (0.45 μm) and concentrated (100 kDa molecular weight cutoff, GE Healthcare) HEK 293T supernatant ...
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bioRxiv - Synthetic Biology 2019Quote: ... and filtered before the cell extract sample was run through a nickel column (GE Healthcare, 11-0033-99). A low-concentration imidazole buffer was run through the column to wash out any non-specific proteins ...
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bioRxiv - Synthetic Biology 2020Quote: ... resuspended in methanol and loaded onto a Sephadex LH-20 (GE Healthcare, Little Chalfont, UK) column for fractionation ...
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bioRxiv - Cell Biology 2022Quote: ... proteins were transferred to previously hydrated with methanol PVDF membranes (Amersham™ Hybond, GE Healthcare) in a vertical tank (Mini-PROTEAN® Tetra Cell ...
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bioRxiv - Microbiology 2020Quote: ... gels were subjected to wet electrotransfer onto methanol-activated polyvinylidene difluoride (PVDF; GE Healthcare, Poland) membranes in 25 mM TRIS ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred to previously hydrated with methanol PVDF membranes (Amersham™ Hybond, GE Healthcare) in a vertical tank (Mini-PROTEAN® Tetra Cell ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils (purity > 97%, viability > 98%) were isolated from healthy heparinised peripheral blood using HetaSep (Stem Cell) and Ficoll-Paque (GE Healthcare), and contaminating erythrocytes were removed by hypotonic lysis as described previously (Wright et al. ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were electroblotted onto methanol-activated Amersham Hybond P0.45 polyvinylidene difluoride (PVDF) membranes (GE Healthcare) in Tris/Glycine transfer buffer (National diagnostics) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatants obtained from the previous step were incubated with equilibriated Strep-Tactin Sepharose beads (28-9355-99, GE Healthcare) for 45 min at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated to the 5 mL mark and the samples were transferred to fresh 15 mL tubes containing 2 mL of 100% Percoll (GE Healthcare catalog no. 17-0891-01). Water was added to the 10 mL mark ...
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bioRxiv - Bioengineering 2019Quote: Cell-free supernatants were prepared by centrifugation and directly loaded (100 mL) onto a 5 mL HiTrap MabSelect column (GE Healthcare). After washing the column with binding buffer (20 mM sodium phosphate ...
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bioRxiv - Biochemistry 2023Quote: ... The GST tag was cleaved off by incubating the washed beads in wash buffer supplemented with PreScission Protease (∼100 mL at 76 mg/mL) (GE Healthcare) overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... The remaining lysate supernatant was incubated for 2 h on a spinning wheel with 200 µl of high-Performance strepTactin Sepharose beads (#28-9355-99, GE healthcare). The beads were collected by centrifugation at 1600 g at 4°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates 30-50 ug were run on SDS-PAGE gels and transferred to nitrocellulose membranes (GE Healthcare). Western blots were conducted using anti-CTLA-4/CD152 (LS C193047 ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was neutralized with 2 M Tris pH7.0 and either diluted first 1:1 with 20 mM phosphate pH7.0 buffer (25 mL milk sample) or loaded directly (100 mL milk sample) onto a Q Sepharose FF (GE Healthcare, Uppsala, Sweden) equilibrated with 20 mM phosphate pH7.2 buffer column at 150 cm/h and 90 cm/h ...
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bioRxiv - Biochemistry 2019Quote: ... 500 mM Imidazole) (0-100% over 50 ml) on an ÄKTA Pure (GE healthcare, USA) at 2 ml/min ...
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bioRxiv - Immunology 2021Quote: ... the transfected cells were supplemented with 100 ml of fresh HyClone SFM4HEK293 medium (GE Healthcare) and sodium butyrate (Reeves et al. ...
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bioRxiv - Plant Biology 2022Quote: ... 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads, GE Life Sciences) were added to each sample in 50% ethanol ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 0.03 % Triton-X 100) and proteins were eluted directly into a 1 mL HisTrap column (GE Healthcare) using purification buffer supplemented with Desthiobiotin 2.5 mM (IBA Solutions For Life Science™) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was added to equilibrated glutathione-Sepharose 4B beads (GE Healthcare; 1 ml/100 g cells) and gently rotated for 4 h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were fixed and stained with 0.4% methylene blue in methanol for 30 min and images acquired with an image analyzer (Amersham Imager 600, GE healthcare).
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bioRxiv - Biochemistry 2021Quote: ... PKAc was immobilized via standard N-hydroxysuccinimide (NHS) / 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) amine coupling on a CM5 (carboxyl methyl dextran) sensor chip (GE Healthcare). Before covalent immobilization of PKAc ...
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bioRxiv - Microbiology 2021Quote: ... using a mixed solution of 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and 50 mM N-hydroxysuccinimide (NHS) in a Biacore T-200 SPR instrument (GE Healthcare), reaching 1000 response units (RU) ...
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bioRxiv - Microbiology 2019Quote: Purified capsids (100 µg) were loaded onto a 5-ml HiTrap™ Heparin-Sepharose HP Column (GE Healthcare), which then washed with Ca2+ and Mg2+-free PBS to remove unbound molecules and eluted with gradually linear increasing concentrations of NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Samples (100 uL) at 1 mg/mL were injected and run over a Superdex 200 GL (GE Healthcare) column at a 0.5 ml/min flow rate ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Microbiology 2020Quote: ... and 10 ug were separated on an 0.8% agarose gel and transferred to a Nylon membrane (GE Healthcare Limited, Buckinghamshire, England) as described previously (36) ...
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bioRxiv - Plant Biology 2019Quote: ... The effluent was then diluted to 50% methanol and concentrated on a DEAE-Sephadex (2ml, GE Healthcare Bio-Sciences AB, Uppsala, Sweden) anion exchange column for the analysis of free IAA and ABA ...