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Citations for GE Life Sciences :
201 - 250 of 4351 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the protein was affinity purified from the supernatant using a 5 mL HisTrap column (GE Healthcare) in 1XPBS pH 7.5 and eluted with 300 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The protein was affinity purified on a Ni2+-column (HiTrap IMAC HP, GE Healthcare Life Sciences). The eluted protein was cleaved with Rhinovirus 3C protease overnight and the cleaved complex was loaded onto a second Ni2+-column and the flow-through collected and further purified by size exclusion chromatography (Superdex 200 ...
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bioRxiv - Cell Biology 2022Quote: ... The soluble fraction was first purified by affinity chromatography using Glutathione Sepharose 4B resin (GE Healthcare) and the GST tag was cleaved by incubating the protein with Thrombin (Sigma Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag fused proteins were purified by affinity chromatography column with GSTrap HP (GE Healthcare) and the His or His-MBP tag fused proteins by nickel chelated HiTrap Chelating HP (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant proteins were purified by Ni2+-affinity chromatography using Histrap HP FPLC columns (GE Healthcare) and a size-exclusion chromatography using a HiLoad 16/60 200 PG column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... The IgG was then purified using protein G affinity chromatography (HiTrap Protein G HP, GE Healthcare) and size-exclusion chromatography (Superdex 200 ...
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bioRxiv - Immunology 2020Quote: ... the cell culture supernatants were harvested and purified by Ni-NTA affinity chromatography (GE Healthcare, USA). Similar to the preparation of rEBOVGP ...
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bioRxiv - Biophysics 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... Endo180-Fc was purified to homogeneity by protein A affinity chromatography using MabSelect PrismA (GE Healthcare) followed by Capto QXP resins (GE BioSceinces ...
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bioRxiv - Microbiology 2022Quote: ... M28 IgG was then purified from the supernatant using Protein A affinity chromatography (GE Healthcare #17549851). IgG was then dialyzed overnight in TBS (150mM NaCl ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified by affinity chromatography using an ÄKTA Explorer FPLC system (GE Healthcare) loaded with a Nickel Sepharose FF 8 mL resin column ...
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bioRxiv - Biophysics 2023Quote: ... Soluble protein was maintained at 4°C and purified using Ni-NTA affinity resin (GE Healthcare). A column wash was performed using pH 7.0 HisA containing 300 mM NaCl ...
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bioRxiv - Biophysics 2023Quote: ... The protein was purified by affinity chromatography using the Ni2+-Sepharose fast flow resin (GE Healthcare), pre-equilibrated with 10 column volumes of buffer A containing 1 mM DTT and gravity loaded with the supernatant from the preceding ultra-centrifugation step ...
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bioRxiv - Biophysics 2023Quote: ... and the protein purified by Ni2+-affinity chromatography using an Äkta FPLC Chromatography System (GE Healthcare). The fusion proteins were eluted with a gradient of 1 M imidazole and dialyzed into 20 mM Tris (pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... The antibodies were purified by affinity chromatography using a Protein A resin (MabSelect SuRe, GE Healthcare), followed by a preparative size exclusion chromatography (SEC) ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... mIgA-604 were purified by anti-light chain affinity chromatography using Capto-L beads (GE Healthcare). dIgA-604 and SIgA-604 were purified using Ni-NTA beads (Smart-Lifesciences ...
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bioRxiv - Plant Biology 2023Quote: ... The antibodies were purified from rabbit serum by affinity chromatography on CH-Sepharose 4B (GE Healthcare) coupled to 6His-CPK3.
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bioRxiv - Microbiology 2024Quote: ... Htc10-LBD was purified using immobilized metal affinity chromatography (IMAC) on ÄKTA Explorer 100 (GE Healthcare) at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... the viral particles were purified using heparin affinity columns (HiTrap Heparin HP; GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filter devices (Millipore ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was collected and purified in a Ni+2NTA affinity column (HisTrap HP– GE Healthcare, USA) according to manufacturer protocol ...
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bioRxiv - Microbiology 2024Quote: ... The mutated proteins were purified from the media using a Protein-A affinity column (GE Healthcare).
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bioRxiv - Cell Biology 2023Quote: ... and the light SILAC labeled sample (transfected with individual constructs) followed by enrichment of biotinylated proteins by Streptavidin Sepharose High Performance affinity resin (GE Healthcare). For all steps ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Overexpressed recombinant proteins in supernatants were purified by AKTA start affinity chromatography system HiTrap Mabselect (GE Healthcare) according to the manufacturer’s instructions (S1 Fig).
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant containing 2F2 or 2E10.E9 IgG was purified via Protein G affinity chromatography (GE Healthcare) and size-exclusion chromatography (Superose 6 Increase 10/300 GL ...
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bioRxiv - Molecular Biology 2021Quote: ... The deglycosylated proteins were then purified by batch affinity chromatography on Glutathione Sepharose 4B resin (GE Healthcare) followed by SEC ...
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bioRxiv - Immunology 2019Quote: ... the samples were purified on nickel-affinity resin (Ni Sepharose High Performance; GE Healthcare, Little Chalfont, UK) after incubation for 30 min at 25 °C and centrifugation of the fraction bound to nickel to pull down the TgMIC-His that physically interacted with TLR-FLAG (16,000 g ...
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bioRxiv - Biophysics 2019Quote: ... purified by immobilized metal affinity chelate chromatography (IMAC) using a Ni2+ Sepharose 6 FastFlow column (GE Healthcare), and then labeled with maleimide-activated LD550 and LD650 fluorophores (Lumidyne Technologies ...
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bioRxiv - Immunology 2021Quote: ... Proteins were purified as above using Ni2+ affinity and size exclusion chromatography (Superdex 75, GE Life Sciences), excluding cleavage of the His6-tags.
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bioRxiv - Biochemistry 2021Quote: AsChi18A was purified by immobilized metal affinity chromatography using a HisTrap FF 5 mL column (GE Healthcare). The periplasmic extract containing AsChi18A was applied on the column using a binding buffer consisting of 20 mM Tris-HCl pH 8.0 and 5 mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged Nrf2 protein and MBP-tagged Rev protein were purified with nickel affinity (HisTrap; GE Healthcare), eluted with imidazole ...
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bioRxiv - Biophysics 2020Quote: ... 100 mM NaCl pH 7.0 and further purified on a 5 ml Heparin affinity column (GE Healthcare) with a 100 ml gradient of 0.1 to 1.5 M NaCl ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant 3CLpro were then purified using HIS Gravitrap Ni-NTA affinity columns (GE Healthcare, Chicago, IL), following the standard protocol (Kim et al. ...
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bioRxiv - Immunology 2021Quote: ... Rhesus sera or plasma antibodies were first purified by affinity chromatography using Protein A Sepharose (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Proteins were purified by immobilized metal-affinity chromatography (IMAC) using pre-packed nickel Sepharose columns (GE Healthcare), or in batch mode on TALON® beads (Takara Bio ...
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bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
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bioRxiv - Plant Biology 2019Quote: ... The expressed protein domains were purified via Ni2+ affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The eluted proteins were subjected to buffer change by PD10 desalting column into ITC-buffer (25 mM BisTris-HCl pH6.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble supernatants were purified by nickel-affinity chromatography (HiTrap Chelating HP column, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD) ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Biochemistry 2021Quote: ... PETISCO was purified by immobilized metal affinity chromatography (IMAC) using Ni2+-chelating beads (HisTrap FF; GE Healthcare). The HisTrap FF column was washed with 20 column volumes of lysis buffer and proteins were eluted with 20 mM Tris/HCl pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was purified via immobilized metal affinity chromatography (IMAC) on a HisTrapTMFF column (GE Healthcare) and eluted with a linear gradient to 40 mM HEPES pH 7.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Soluble supernatants were purified by affinity chromatography (StepTag or HiTrap Chelating HP columns, GE Healthcare Life Science) using an NGC Quest 10 Plus Chromatography System (BIO-RAD ...
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bioRxiv - Microbiology 2021Quote: ... Secreted proteins were purified from the culture supernatants using a protein A+G affinity column (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... MBs were purified by affinity chromatography using a HiTrap Protein A HP column (GE Healthcare, Chicago, IL) and eluted with 20 mM Tris pH 8.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies were affinity-purified on Fy-4 immobilized on a HiTrap NHS- activated HP column (GE Healthcare). Antibodies were eluted using Pierce Gentle Ag/Ab Elution Buffer (ThermoFisher).