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Citations for GE Life Sciences :
101 - 150 of 731 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... nitrocellulose Hybond-C extra (GE Healthcare) membranes were spotted with 0 to 200 μM of PA (Sigma P-9511 ...
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bioRxiv - Biochemistry 2021Quote: ... of L-enantiomeric peptides CDP1 and CDP7 binding to SARS-CoV-2 3CLpro was determined by Surface Plasmon Resonance Spectroscopy (SPR) using a Biacore 8K instrument (GE Healthcare, Uppsala, Sweden). The peptides were immobilised on two separate channels on a series S CM-5 sensor chip (Cytiva ...
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bioRxiv - Molecular Biology 2019Quote: ... coli BL21(DE3) pLysS and purified from inclusion bodies via Sephacryl S-200 gel filtration (GE Healthcare). Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... coli and after cell disruption the cleared lysate was applied to a GSTrap chromatography column (GE Healthcare). After the on-column cleavage with 3C protease (prepared according to in house protocol) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Biophysics 2021Quote: SPR measurements were recorded at either 25°C or 35°C using a Biacore T200 system (GE Healthcare). All experiments were performed using an SA Series S Sensor Chip (GE Healthcare) ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 and were purified by immunoprecipitation with glutathione sepharose 4B beads (Cat: 45-000-139, GE Healthcare). Each reaction with 30 μl final volume contained 50 ng Wheat E1 protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Neuroscience 2020Quote: ... Placement in the spinal cord was confirmed with a commercially-available ultrasound system (LOGIQ e Vet, GE Healthcare, Fairfield, CT) using a linear array 4-10 MHz transducer (8L-RS) ...
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Synaptic Zn2+ potentiates the effects of cocaine on striatal dopamine neurotransmission and behaviorbioRxiv - Neuroscience 2021Quote: ... Slides were dried under stream of cool air and placed on BAS-IP TR 2025 E Tritium Screen (GE Healthcare) for 5-days and imaged using a phosphor imager (Typhoon FLA 7000 ...
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bioRxiv - Bioengineering 2020Quote: Vascular patency was checked using the color doppler mode of an ultrasonic device (LOGIQ e Ultrasound; GE Healthcare, Fairfield, USA). Angiography was also performed with a bolus intravenous injection of 2 ml/kg iohexol (Omnipaque™ ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) and were isolated under native purification conditions using a 5 HisTrap High Performance Column (GE Healthcare) with imidazole gradient (10-250 mM ...
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bioRxiv - Biochemistry 2021Quote: ... coli and purified using Ni-NTA affinity resin (Quiagen) and followed with SEC (Superose 6 Increase, GE Life Sciences), as previously described10 ...
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bioRxiv - Neuroscience 2020Quote: ... All GST fusions were expressed in BL21 Escherichia coli and purified with glutathione-Sepharose 4B (GSH beads; GE Healthcare). 100µM of biotinylated- Aβ oligomer and 200µg of GST ...
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bioRxiv - Neuroscience 2022Quote: ... the Aβ42 peptide was recombinantly expressed in BL21*(DE3) pLysS Escherichia coli and purified with DEAE-Sepharose (GE Healthcare). To get rid of large Aβ42 aggregates ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was diluted two-fold in buffer E + 1 mM DTT + 150 mM NaCl and applied to a MonoS column (GE Healthcare) pre-equilibrated in buffer E + 150 mM NaCl + 1 mM DTT + 0.5 mM EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... Following 3C protease treatment CDK2-cyclin E was buffer exchanged using SEC (Superdex 200 10/300) and then loaded onto a GST-trap column (5ml, GE Healthcare) and then onto an amylose resin (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein quality was assessed by SDS-PAGE and fractions containing RNase E were pooled and loaded onto a butyl sepharose column (GE Healthcare) equilibrated in high-salt buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Physiology 2020Quote: Echocardiography was performed at baseline and following the induction of pulmonary hypertension (PH) (6S probe and Logiq E console, GE Healthcare). Images were acquired in the supine position via a parasternal window.
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Cell Biology 2021Quote: ... 4 (excepting E) were performed at room temperature using the ring TIRF light path of a DeltaVision OMX SR microscope (GE Healthcare) with a 60x/1.42 numerical aperture (NA ...
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bioRxiv - Neuroscience 2023Quote: ... The course of the radial nerve just above the elbow was visualized using ultrasound (LOGIQ e, GE Healthcare, Chicago, IL, USA). A high-impedance tungsten recording electrode was inserted percutaneously and with ultrasound guidance it was inserted into the nerve ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Microbiology 2024Quote: ... which was imaged by overnight exposition of a BAS-IP MS 2040 E phosphor screen (Cytiva) scanned using a Typhoon FLA 9500 (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... The solution was dialyzed against Buffer C overnight at 4 °C and applied on a Resource Q column (GE Healthcare) that was equilibrated with Buffer C ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biophysics 2020Quote: ... onto a Hybond-C extra membranes (GE Healthcare). Membrane strips were blocked with 3% fatty acid-free BSA (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... at 25°C using Biacore T200 (GE Healthcare). The 6×His-tagged AVR-PikD (ligand ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was Hybond-C nitrocellulose (GE Healthcare), and transfer was performed in 25 mM Tris ...
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bioRxiv - Immunology 2022Quote: ... at 20 °C in HBS-EP+ (GE Healthcare) running buffer ...
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bioRxiv - Genomics 2023Quote: ... transferred onto Hybond C nitrocellulose membrane (GE Healthcare), probed with primary antibodies anti-RRM diluted 1:300 ...
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bioRxiv - Biophysics 2020Quote: ... coli BL21(DE3) cells and purified from the cell lysate using Glutathione-Sepharose 4B resin (Cytiva, Tokyo, Japan (GE Healthcare)) ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) carrying the plasmid pPyoS5 with initial purification using a cation exchange column (CM16/10 FF, GE Healthcare; buffers ...
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bioRxiv - Microbiology 2021Quote: ... recombinant GST-preS1 was produced in Escherichia coli BL21 cells and purified using glutathione sepharose 4B (GE Healthcare, United States). Ten micrograms of GST or GST fusion proteins and cell lysates were incubated at 4 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... monomeric transthyretin (MTTR) was expressed in Escherichia coli and purified by affinity in a HisTrap column (GE Healthcare Life Science). Peak fractions were combined and further purified by size exclusion chromatography on a Superdex S75 prep grade column (GE Healthcare Life Science ...
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bioRxiv - Microbiology 2022Quote: ... coli supernatant was directly applied onto a 1-ml Ni2+ ion chelating poly-histidine affinity column (HisTrap HP, GE Healthcare) equilibrated with buffer A (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... MALX-E-NirF was purified by loading the cleared cell lysate onto a 5ml MBPtrap HP column (GE Healthcare, Chicago, United States) which was eluted with a gradient to 20 mM maltose ...
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bioRxiv - Systems Biology 2022Quote: ... cells were fixed and immunostained for DENV E using the procedure as previously described.5 Images were acquired using the IN CELL Analyzer 3000 (INCA3000, GE Healthcare, USA) using a 40X magnifying objective allowing for nine images per well ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then collected by vacuum filtration onto glass microfiber filters (grade GF/C, 125 mm in diameter, pre-combusted at 460°C for > 5 h, GE Healthcare Whatman). The filters were transferred to 50 mL centrifuge tubes (Sarstedt ...
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bioRxiv - Cell Biology 2022Quote: ... Extract was cleared by centrifugation at 186.000 x g for 1 h at 4°C and then incubated at 4°C with Glutathione Sepharose™ 4B resin (GE Healthcare) for 3h ...
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bioRxiv - Cell Biology 2020Quote: ... 28°C and purified on HisTrap columns (GE Healthcare) by Imidazole gradient elution on AKTA Prime according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C onto 0.2uM PVDF membranes (GE Healthcare Biosciences). After transfer ...
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bioRxiv - Genomics 2024Quote: ... transferred onto a Hybond-C nitrocellulose membrane (GE Healthcare), then blocked with TBST containing 5% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) pLysS and purified under native conditions by ion exchange chromatography using Q-Sepharose Fast Flow (GE Healthcare, Sweden) as described previously [66] ...