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Citations for GE Life Sciences :
1 - 50 of 4115 citations for SARS Coronavirus Envelope Protein E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... coli 55244 cells (ATCC) and purified using Protein L (GE Healthcare) gravity flow affinity chromatography as published previously29 ...
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bioRxiv - Immunology 2023Quote: ... coli proteins using a size exclusion column (Cytiva or GE Healthcare). For biotinylation of the protein ...
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bioRxiv - Immunology 2021Quote: ... Purification of E-301 and E-301-LOF was performed directly from transfection harvests using a HiTrap Protein A affinity column (GE Healthcare) and eluted with 1M arginine pH 3.9 ...
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bioRxiv - Plant Biology 2022Quote: ... coli DH5a and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Microbiology 2020Quote: ... blocked for 1 hour and immuno-detected using Anti-RecA (E. coli) antibody (1000x dilution) (gift from Mike Cox, (52)) and analyzed using an ECL Prime detection kit (GE Healthcare). Densitometry was performed using ImageJ (https://imagej.nih.gov/ij/)
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bioRxiv - Plant Biology 2023Quote: ... coli Rosetta/DE3 and the proteins purified using glutathione resin (GE Healthcare, USA) as described (Fliegmann et al. ...
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bioRxiv - Biochemistry 2021Quote: ... isolated E coli membranes with overexpressed MgtA were solubilized in 1% β-dodecyl maltoside (β-DDM) prior to Histrap HP (GE Healthcare) purification equilibrated in (25 mM HEPES pH 7.0 ...
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bioRxiv - Plant Biology 2023Quote: ... coli or insect cells as fusion proteins subcloned into pGEX-6P-1(GE Healthcare) or te pFastBac-1 vector (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Neuroscience 2020Quote: ... This was expressed in E .coli strain NiCo21 (DE3) using conditions previously described(96) and purified by IMAC using NiHP (GE Healthcare Life Sciences) and AEC using QHP (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified from Escherichia coli by glutathione-Sepharose 4B beads (GE Healthcare) and then washed with high salt buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... coli M55244 cells (ATCC) and purified using Protein L resin (GE Healthcare Cat no. 17547802) with gravity flow affinity chromatography ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike protein in dPBS was applied to a 1-ml HiTrap heparin-Sepharose column (GE Healthcare). The column was washed with 5 ml of dPBS and bound protein was eluted with a gradient of NaCl from 150 mM to 1 M in dPBS.
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bioRxiv - Plant Biology 2023Quote: ... The protein was produced in Escherichia coli strain Rosetta and purified with nickel Sepharose media (GE Healthcare). EMSA was performed according to the reference guide of the LightShift Chemiluminescent EMSA kit (Thermo Scientific 20148) ...
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bioRxiv - Bioengineering 2020Quote: Binding of SARS-CoV-2 RBD to ACE2 –Fc and ACE2mod–Fc fusion proteins were measured using a Biacore T200 instrument (GE Healthcare). Fusion proteins were first immobilized at a coupling density of ∽1000 response units (RU ...
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bioRxiv - Microbiology 2021Quote: Kinetic measurements of the DH1047 Fab binding to SARS-CoV and RsSHC014 spike proteins were obtained using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Immunology 2022Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 RBD protein (SinoBiological) were analyzed by SPR (Biacore T200, GE Healthcare). Specifically ...
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bioRxiv - Immunology 2022Quote: SPR screening and affinity measurements of monoclonal Fabs binding to SARS-CoV-2 spike proteins were performed using a Biacore S200 instrument (Cytiva, formerly GE Healthcare) in HBS-EP+ 1X running buffer ...
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bioRxiv - Biochemistry 2021Quote: The binding affinities of full IgG antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2021Quote: The binding affinities of antibodies to SARS-CoV-2 spike protein were determined using surface plasmon resonance (SPR) and a BIAcore T200 instrument (GE Healthcare) at 25°C ...
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bioRxiv - Immunology 2022Quote: ... The ACE2 receptor or SARS-CoV-2 spike-specific antibodies (CR3022 or S309) were immobilized on the protein A sensor chip (GE Healthcare) at a ligand capture level of ∼100 RU ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 cells as described previously.13 The protein was purified using glutathione-Sepharose 4B beads (GE Healthcare) concentrated and flash-frozen in GST buffer [25 mM Tris (pH 7.5) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 S-2P and SARS-CoV-2 HexaPro S were purified over a Superose6 Increase column (GE Life Sciences).
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bioRxiv - Cell Biology 2023Quote: ... Escherichia coli BL21 (GE Healthcare) was transformed with each GST-fusion protein construct and the expression of GST-fusion protein was induced by 0.1 mM isopropyl-β-D-thiogalactoside (IPTG ...
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bioRxiv - Cell Biology 2019Quote: ... and 0.5 mM PMSF. Mouse anti-Spt6 antibody (25C3; E. Kremmer/A. Schepers) was coupled to Protein A-Sepharose CL-4B (GE Healthcare) beads and stored at 4°C until actual pulldown ...
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bioRxiv - Genomics 2024Quote: ... WSC3-e cells (1.6 µg protein µL-1) was loaded onto a Superdex 200 10/300 column (GE Healthcare, Chicago, USA), which was equilibrated with a buffer containing 50 mM Tris-HCl (pH 7.84) ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were expressed as GST fusion proteins in Escherichia coli DH5α and were purified with glutathione-Sepharose beads (GE Healthcare), as described (Otera et al. ...
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bioRxiv - Cell Biology 2020Quote: ... e Image-Quant LAS 4000 (GE Healthcare). Densitometry analysis of the gels was performed using the Image J software (version 1.37) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli knockout collection (Dharmacon, GE Healthcare). Hedamycin was obtained from the National Cancer Institute’s Developmental Therapeutic Program (NCI DTP ...
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bioRxiv - Plant Biology 2023Quote: ... coli vector pGEX-2TK (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH5α strain and purification of GST fusion proteins was achieved by incubation with Gluthatione Sepharose 4B matrix (GE Healthcare, Buckinghamshire, UK). Sumo proteins were kept bound to matrix ...
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bioRxiv - Cell Biology 2021Quote: ... coli and/or immobilized CaM (GE Healthcare), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... coli BL21 cells (GE Healthcare, Freiburg, Germany) expressing GST- or 6xHis fusions were grown to an OD600=0.8 at 37°C in 2YT or LB (6xHis fusions) ...
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bioRxiv - Plant Biology 2022Quote: ... coli using Glutathione Sepharose beads (GE healthcare). The GST-tagged protein-bead complexes were incubated with 2 μg of MBP-tagged proteins in 1 mL of pull-down buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 Gold (GE Healthcare, Freiburg, Germany) was diluted into 500 ml of SB medium and incubated at 37°C to an OD600 of 0.8 ...
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bioRxiv - Biochemistry 2019Quote: ... coli and coupled to CNBr-beads (GE Healthcare). The specificity of custom-made antibodies was confirmed by western blotting against human cell lysate after RNAi depletion of target proteins as described previously (Cota et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using ÄKTA pure (GE Healthcare) with HiTrap Blue HP ...
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bioRxiv - Molecular Biology 2020Quote: ... coli and purified by Glutathione Sepharose 4B (GE Healthcare), according to the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli BW25113 was purchased from Dharmacon (GE Healthcare). ompC ...
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bioRxiv - Biochemistry 2020Quote: ... coli was performed on an ÄKTApurifier instrument (GE Healthcare) with the modules Box-900 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli and purified with Glutathione SepharoseTM 4B (GE Healthcare). The glycosyltransferase activity assay was carried out with the conditions described by Hou et al ...
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bioRxiv - Neuroscience 2021Quote: ... the E domain was subcloned into pGEX-6P-1 (GE healthcare). The GST-gephyrin E domain fusion protein was purified from E ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 RBD or NL63 RBD was immobilized to a CM5 sensorchip (GE Healthcare) using a Biacore T200 (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... coli cells were transformed with pGEX-4T-3 (GE Healthcare) containing the coding sequences of fascin ...
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bioRxiv - Molecular Biology 2020Quote: ... coli strain BL21 and purified using His-trap (GE Healthcare), Hi-Trap Q (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... coli BL21 by batch purification using glutathione sepharose (GE Healthcare, Chicago ...
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bioRxiv - Cell Biology 2021Quote: ... coli JM109 and purified with glutathione-Sepharose beads (GE Healthcare) by the standard protocol ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3) using pGEX4T-3 vector (GE healthcare, IL).