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Citations for GE Life Sciences :
401 - 450 of 1244 citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Horseradish Peroxidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Single-cycle kinetics analysis of CD4-Ig binding to the 16055-ConM-v8.1 SOSIP with C-terminal Spy-tag-2 (ST2) was performed at 25°C on a Biacore T100 (GE Healthcare). 1X HBS-EP containing 10 mM HEPES ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... or sheep anti-mouse IgG (GE Healthcare, cat #NA931, RRID:AB_772210, 1:10,000) in TBS-T containing 5% milk for 2 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies used were sheep anti-mouse HRP (GE healthcare; 1:5000) and donkey anti-rabbit (GE healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-linked sheep anti– mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... or HRP-liked sheep anti-mouse IgG (GE Healthcare, #NA931V, 1:10,000) together with Western Lightning Plus-ECL (Perkin Elmer ...
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bioRxiv - Neuroscience 2023Quote: ... Sheep anti-Mouse IgG-HRP (1:3,000, GE Healthcare, Cat# NXA931, RRID:AB_772209), donkey anti-Rabbit IgG-HRP (1:2,000 ...
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Persistence of learning-induced synapses depends on neurotrophic-priming of glucocorticoid receptorsbioRxiv - Neuroscience 2019Quote: ... Immunoreactivities (antibodies listed in Table S1) were revealed by chemiluminescence (GE Healthcare) and densitometric analysis of grayscale digital images using Chemidoc Touch (Bio-Rad Laboratories).
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bioRxiv - Microbiology 2020Quote: SΔTM and RBD glycoproteins were resolved on SDS-PAGE and transferred onto a nitrocellulose membrane (Amersham Protran, GE Healthcare). Lysates of 293T cells transfected with plasmids encoding full-length spike glycoproteins was also separated by SDS-PAGE for WB ...
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bioRxiv - Microbiology 2023Quote: ... F045 and TriHSB.2 constructs contain C-terminal 6xHis tags and were purified by passage over a HisTrapFF column (GE Healthcare), followed by size exclusion chromatography using a Superdex 200 increase 10/300 column (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... supplemented with 10% FCS (GE Healthcare) and antibiotics (100 U ml−1 penicillin and 100 µg ml−1 streptomycin) ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells were passaged in HyClone DMEM (GE Healthcare Systems, Marlborough, MA) supplemented with 5% FBS and 100 units pen/strep per ml (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cell lysates were incubated with 20 µL IgG Sepharose (GE Healthcare) or as a control Sepharose without IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Horseradish peroxidise (HRP)-conjugated secondary antibodies (GE Healthcare) were detected using enhanced chemiluminescence (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... peroxidase-linked species-specific F(ab’)2 fragment (from donkey) (1:10,000 in 3% skim milk/TBST, NA9340, GE Healthcare) Mouse TrueBlot ULTRA ...
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bioRxiv - Cell Biology 2019Quote: ... HRP-conjugated anti-rabbit antibody (sheep polyclonal, used at 1:3000, GE Healthcare) and HRP-conjugated anti-chicken antibody (donkey polyclonal ...
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bioRxiv - Neuroscience 2020Quote: ... either sheep anti-mouse-HRP (1:5000, NA931V, GE Healthcare, Chicago, IL, USA) or donkey anti-rabbit-HRP (1:5000 ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... peroxidase-conjugated anti-rabbit (GE Healthcare), for 1 h at room temperature in TBST (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... Spikes were further purified by Superose 6 increase 10/300 (GE healthcare) size-exclusion column in a buffer containing 2mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... The Spike protein was purified on an Akta Express system (GE Healthcare) using a 5mL His trap FF GE Healthcare column in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... for the spike protein and a Superdex 75 10/300 (GE Healthcare) for the RBD and the RBD triple mutant showed a monodispersed and single peak with the expected molecular mass (Supplementary Figure 1).
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Immunology 2021Quote: ... 10% (v/v) FCS (Hyclone, GE Healthcare) and 0.1 mg/ml penicillin/streptomycin (15140122 ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with biotinylated sheep anti-mouse (1:200, RPN1001V, GE Healthcare) followed by peroxidase labeled streptavidin (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... and HRP-linked Sheep Anti-Mouse IgG (1:2000; GE Healthcare Life Sciences, NAG31V). For the analysis of p65 NFκB localisation and phosphorylation ...
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bioRxiv - Neuroscience 2022Quote: ... HRP-conjugated sheep anti-mouse IgG (Cat# NA931, RRID: AB_772210, GE Healthcare, Buckinghamshire, UK) and donkey anti-rabbit IgG (Cat# NA934 ...
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bioRxiv - Developmental Biology 2019Quote: Secondary antibodies included HRP-conjugated sheep anti-mouse IgG (GE Healthcare, NA931V, lot 9682503), HRP-conjugated donkey anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: Time lapse imaging of cultured primary neurons (in Schneider’s/FCS) and fibroblasts (in Ham’s F-12/FCS) was performed on a Delta Vision Core (Applied Precision) restoration microscope using a [100x/1.40 UPlan SAPO (Oil)] objective and the Sedat Quad filter set (Chroma #89000) ...
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bioRxiv - Microbiology 2020Quote: ... Spike protein was finally purified using Superose 6 HR10/300 column (GE Healthcare) pre-equilibrated with buffer containing 20 mM HEPES (pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... Anti-rabbit horse-radish peroxidase (GE healthcare) was used as the secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... Secondary peroxidase labeled α-mouse (GE Healthcare), α-rabbit (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Molecular Biology 2022Quote: ... sheep anti-mouse-IgG and anti-rat-IgG (all from GE Healthcare, Chicago, IL, USA). The antibodies were detected with Enhanced Chemiluminescence kit (Thermo Fischer Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare). Mouse TrueBlot® ULTRA ...
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bioRxiv - Cell Biology 2023Quote: ... Canonical secondary antibodies used were sheep anti-mouse or donkey anti-rabbit HRP (GE Healthcare). Immunoreactivity was visualized with Immobilon Forte Western HRP substrate (Millipore).
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bioRxiv - Immunology 2022Quote: ... Trimeric spike protein was further purified by Superose 6 size-exclusion chromatography (GE Healthcare) in 10 mM Tris pH 8 ...
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bioRxiv - Immunology 2022Quote: ... while the D614G VFLIP spike was purified on a StrepTrap XT column (GE Healthcare), according to the manufacturer’s protocols ...
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bioRxiv - Biophysics 2023Quote: Suspension HEK HEK293S GnTI- cells were grown and maintained in HyClone TransFx-H (GE Healthcare) media supplemented with 1% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Peroxidase-linked anti-mouse immunoglobulin G and peroxidase-linked anti-rabbit immunoglobulin G (both GE Healthcare Bio-Sciences, Pittsburgh, PA). Alexa Fluor 594-conjugated Donkey Anti-Mouse IgG (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with peroxidase-conjugated secondary antibodies (GE Healthcare) and visualised with chemiluminescent substrates (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2020Quote: ... Peroxidase-conjugated secondary antibodies were from GE Healthcare. Alexa-conjugated secondary antibodies were from Life Technologies.
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The secondary antibodies used for Western blot detection were: sheep anti-mouse HRP (GE Healthcare #NA931), donkey anti-rabbit HRP (GE Healthcare #NA934V).
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bioRxiv - Neuroscience 2023Quote: ... The following secondary antibodies were used: sheep anti-mouse (HRP-conjugated, GE Healthcare, NXA931V, 1:10000), donkey anti-rabbit (HRP-conjugated ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibody used in Western blotting was HRP sheep anti-mouse IgG (GE Life Sciences NA931V).
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bioRxiv - Immunology 2020Quote: ... Fc was removed via ProteinA Sepharose incubation (GE Healthcare) at 4°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% fetal calf serum (FCS, GE Healthcare Life Sciences) and 500 U/mL penicillin-streptomycin (Gibco ...