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Citations for GE Life Sciences :
351 - 400 of 5074 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... coli and/or immobilized CaM (GE Healthcare), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... coli BL21 cells (GE Healthcare, Freiburg, Germany) expressing GST- or 6xHis fusions were grown to an OD600=0.8 at 37°C in 2YT or LB (6xHis fusions) ...
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bioRxiv - Plant Biology 2022Quote: ... coli using Glutathione Sepharose beads (GE healthcare). The GST-tagged protein-bead complexes were incubated with 2 μg of MBP-tagged proteins in 1 mL of pull-down buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 Gold (GE Healthcare, Freiburg, Germany) was diluted into 500 ml of SB medium and incubated at 37°C to an OD600 of 0.8 ...
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bioRxiv - Plant Biology 2022Quote: ... and the mixture of MoCel10A-His and OsCBMIP-His preincubated at 4 °C for 30 min were applied on Superdex G-75 column (GE Healthcare, Buckinghamshire, UK) equilibrated with sodium phosphate buffer (50 mM ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... were coupled 2 h at RT to protein A Sepharose beads (GE Healthcare). Coupled beads were covalently crosslinked using dimethyl pimelimidate according to standard procedures ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using 2-D quant kit (GE healthcare, Cat# 80648356), by following manufacturer’ instruction ...
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bioRxiv - Molecular Biology 2019Quote: ... Stoichiometric amounts of each core histone were incubated together under high salt conditions (2 M NaCl) and the resulting histone octamer purified using a Superdex 200 gel filtration column (GE Healthcare). Purified 147bp DNA carrying the 601 nucleosome-positioning sequence was a kind gift from the Brockdorff lab ...
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bioRxiv - Biophysics 2021Quote: ... After dialysis the histones in 2 M NaCl were concentrated to a volume of 1 mL and purified using an Superdex 200 column (GE Healthcare) on an FPLC (Agilent) ...
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bioRxiv - Bioengineering 2020Quote: ... The ligase was inactivated with 2 M NaCl and the sample was injected into a Sephacryl S-1000 size exclusion column (GE Healthcare) to remove excess oligos and DNA ligase.
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bioRxiv - Biophysics 2019Quote: ... The eluted Sox2 and Oct4 were refolded by dialyzing to 2 M urea and then to 0 urea using a desalting column (GE healthcare). Further purification was carried out by gel filtration using a Superdex 200 10/300 GL column (GE Healthcare) ...
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bioRxiv - Biophysics 2020Quote: ... which contains DnaK protein was purified first with ion exchange chromatography using 0 to 1 M NaCl in a HiTrap column (HiTrap, GE Healthcare, Germany). Primary fraction of DnaK was eluted in 150 mM NaCl which was further purified using size exclusion chromatography using a superdex 200 column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... cells were lysed using an M-110S Microfluidizer (Microfluidics) and proteins were purified using glutathione chromatography according to the manufacturer’s instructions (GE Healthcare Life Sciences). To confirm the size and purity of purified recombinant protein ...
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bioRxiv - Microbiology 2019Quote: ... The C-terminal His6-tagged protein was purified from the supernatant using Co2+-affinity chromatography (His GraviTrap™ TALON®, GE Healthcare Life Sciences Co., Ltd.) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Immunology 2022Quote: ... while the D614G VFLIP spike was purified on a StrepTrap XT column (GE Healthcare), according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2021Quote: ... Purified 6x-His-SUMO or 6x-His-SUMO-Pat1 were coupled to NHS-activated Sepharose 4 fast flow resin (GE Healthcare, 17-0906-01) in ligand coupling buffer (200 mM NaHCO3 ...
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bioRxiv - Molecular Biology 2022Quote: ... was purified and washed on His-Trap columns (GE Healthcare) with buffer Ac (50 mM Tris pH 7.9 ...
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bioRxiv - Biophysics 2022Quote: ... and purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was confirmed via a Superdex 200 column on an AKTA Pure system (GE Healthcare).
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bioRxiv - Biochemistry 2021Quote: ... A His-Trap crude FF column (GE Healthcare Life Sciences) was used for this purification ...
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bioRxiv - Biochemistry 2019Quote: ... coli and coupled to CNBr-beads (GE Healthcare). The specificity of custom-made antibodies was confirmed by western blotting against human cell lysate after RNAi depletion of target proteins as described previously (Cota et al. ...
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bioRxiv - Biochemistry 2023Quote: ... coli and purified using ÄKTA pure (GE Healthcare) with HiTrap Blue HP ...
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bioRxiv - Biochemistry 2022Quote: ... The GST tag was cleaved with PreScission Protease (GE Healthcare) overnight ...
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bioRxiv - Cell Biology 2022Quote: ... The tags were cleaved off by PreScission protease (GE Healthcare) and the proteins were further purified by sizeexclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2023Quote: ... The GST tag was cleaved by PreScission Protease (GE Healthcare) treatment over 12 hrs at 4°C with the progress of reaction monitored by SDS-PAGE ...
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bioRxiv - Immunology 2020Quote: ... MERS-CoV S1 was purified over a Superdex200 Increase column (GE Life Sciences). SARS-CoV-2 S-2P ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered (0.45 μm) and the target protein purified via immobilized metalion affinity chromatography (IMAC) using a 5 mL HisTrap™ HP column (GE Healthcare, UK). The lysis buffer (50 mM Tris/HCl ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations were determined using the 2-D Quant kit (GE Life Sciences 80648356). Samples were reduced (5 mM DTT ...
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bioRxiv - Plant Biology 2023Quote: ... The extracted protein solution was quantified using the 2-D Quant Kit (GE Healthcare) following the manufacturer’s instruction.
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bioRxiv - Plant Biology 2019Quote: Samples each containing 2 μg Chl a of ΔpsbO-PSII-M and ΔpsbO-NRC were precipitated using the 2D Cleanup Kit (GE Healthcare, Chicago, IL) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... and affinity purified over a His-Trap HP column (GE Healthcare). The proteins were gel-filtered and their size was validated via a Superdex 200 column on an AKTA Pure system (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: His-tagged HexaPro was immobilized to a NiNTA sensorchip (GE Healthcare) to a level of ∼500 response units (RUs ...
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bioRxiv - Cell Biology 2020Quote: ... Equilibrated a 5 ml His-Trap HP column (GE Life Sciences) with the lysis buffer lacking protease inhibitors ...
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bioRxiv - Biochemistry 2022Quote: ... His-GST-ARS2 was applied onto a GST resin (GE Healthcare) and His-MBP-ZFC3H1 onto an Amylose resin (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE healthcare). Histones were eluted by a salt gradient from 200 mM to 600 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μl of His Mag Sepharose® Ni beads (GE Healthcare) were equilibrated with equilibration buffer containing 20 mM sodium phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Non-acetylated CobB was purified on His-Trap columns (GE healthcare) as described above however ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the supernatants were mixed with His GraviTrap affinity column (GE Healthcare) and eluted in elution buffer (20 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cell Biology 2020Quote: ... and then incubated for 3 hours with His beads (GE Healthcare) prewashed in His binding buffer ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDwt or RBDmut proteins were equilibrated in PBS and kept at −20°C ...
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bioRxiv - Immunology 2021Quote: ... and purified by passing His-Trap HP column (GE Healthcare, USA). Collected RBDs proteins were equilibrated in PBS and kept at -20°C.
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bioRxiv - Biochemistry 2023Quote: ... before loading onto a Hi-Trap SP-Sepharose column (GE Healthcare). Histones were eluted by a salt gradient from 200 to 600 mM NaCl ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Buffer was added to the supernatants to a final concentration of 0.01 M HEPES/0.2 M NaCl (pH 7.4) and passed through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.2 ...
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bioRxiv - Immunology 2023Quote: ... The peak of properly refolded HLA-G2 was collected and buffer exchanged into HBS-EP (0.01 M HEPES pH 7.4, 0.15 M NaCl, 3 mM EDTA and 0.005% [v/v] Surfactant P20, GE Healthcare) for surface plasmon resonance (SPR ...
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bioRxiv - Neuroscience 2021Quote: ... A Tracerlab FX M module (GE Healthcare) was used for automated methylation ...
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bioRxiv - Cancer Biology 2022Quote: ... M-016083-00-0005 (GE Healthcare Dharmacon). siRNA non-targeting pool #2 (GE Healthcare Dharmacon ...
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bioRxiv - Molecular Biology 2020Quote: ... coli and purified by Glutathione Sepharose 4B (GE Healthcare), according to the manufacturers’ instructions.
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bioRxiv - Cell Biology 2020Quote: ... coli using the pGEX-5X-3 vector (GE Healthcare). The antiserum was affinity purified using the antigen coupled to Dyna M-280 Streptavidin beads (Life Technologies).