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Citations for GE Life Sciences :
301 - 350 of 638 citations for Rubella virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The pellet was added to lysed HEK cells prior to virus purification through heparin affinity columns (HiTrap Heparin HP, GE Healthcare, Uppsala, Sweden) and concentrated using Amicon Ultra-4 centrifugal filters (Millipore ...
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bioRxiv - Biophysics 2021Quote: ... The protein was purified from the lysate using Ni2+-NTA column of [KTA Pure (GE healthcare). For in vitro biotinylation of the polyprotein ...
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bioRxiv - Microbiology 2021Quote: ... lysates were incubated 30 min with chelating Sepharose Fast Flow beads charged with Ni2+ (GE Healthcare). Beads were then successively washed in the washing buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The cell lysate was incubated with 10 ml of Glutathione Sepharose 4B resin from GE Healthcare, equilibrated with the same buffer for 2 hours ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Bacterial cell lysates were then loaded onto a HiTrap Q HP anion exchange column (GE Healthcare) and were then equilibrated with 20 mM Tris/0.5 mM EDTA (pH 8.3 ...
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bioRxiv - Molecular Biology 2022Quote: ... H-NS containing cell lysate was directly applied to a HiTrap Heparin HP column (GE Healthcare) equilibrated with Binding Buffer with 130 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... Protein was then affinity purified from lysate using a StrepTrap HP 5 mL column (GE Healthcare) in binding buffer (100 mM tris-HCl ...
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bioRxiv - Microbiology 2019Quote: ... The cell lysate was then passed through a 1ml HisTrap column (GE healthcare, Little Chalfont, UK). For every 2 ml of cell lysate passed though the column ...
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bioRxiv - Biochemistry 2019Quote: ... The resultant lysate was loaded onto a 7.5 mL nickel SepharoseTM fast flow column (GE Healthcare), pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were separated on a Superose 6 10/300 column (Ge Healthcare, Waukesha, WI, USA) pre-equilibrated with 20mM Tris–HCl pH7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysate was applied to a 5 ml HiTrap HP Q column (GE Healthcare Biosciences). The flow-through ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 100 μl of Streptavidin-Sepharose beads (GE Healthcare; 17-5113-01) for 3 h at 40°C ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was cleared by ultracentrifugation and supernatant was incubated with GST-4B beads (GE Life Sciences) for 1H ...
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bioRxiv - Cell Biology 2021Quote: ... Cleared lysate was incubated with 1 mL IgG Sepharose beads suspension (6 Fast Flow, GE Healthcare) pre-rinsed with buffer for 20 ml lysate on a rotating wheel at 4°C for 1 h ...
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bioRxiv - Plant Biology 2020Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The MBP-OsHIPP19-HMA/6xHis-SUMO-AVR-PikF complex was step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates and samples were separated by SDS–PAGE and transferred to PVDF membrane (GE Healthcare) using wet transfer and Towbin blotting buffer (25 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... The lysate soluble fraction was loaded onto a 5 mL HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were electrophoresed in a 10% Acrylamide gel and transferred onto a nitrocellulose membrane (GE Healthcare) using a semi-dry blotter (BioRad) ...
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bioRxiv - Biophysics 2022Quote: ... The clarified lysates were loaded first with 2 ml of DEAE Sepharose fast flow (GE Healthcare) and the elutes obtained by a gradient of 100-500 mM NaCl in 50 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Biophysics 2021Quote: ... The cleared cell lysate was applied to a HisTrap FF Ni-NTA affinity column (GE healthcare) equilibrated with HisA buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The cleared lysates were loaded onto Ni-Sepharose beads and nutated for ∼1 h (GE Healthcare). Beads were washed twice with denaturing buffer and hexa-histidine-tagged proteins were eluted with the same buffer supplemented with 500 mM imidazole ...
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bioRxiv - Biophysics 2020Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg of cell lysate was incubated with 40 μl of glutathione-Sepharose beads (GE Healthcare) at 4°C ...
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bioRxiv - Biophysics 2019Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the cleared lysate was loaded onto a 5 ml HisTrap FF column (GE Healthcare, Chicago, U.S.A.) connected to an Äkta Purifier FPLC system (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... The lysate soluble fraction was loaded onto a 5 ml HiTrap chelating HP column (GE Healthcare) immobilized with 100 mM cobalt chloride and equilibrated with the lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Lysate was clarified by centrifugation and loaded onto HisTrap™ High-Performance 5 mL (GE Healthcare) columns using a peristaltic pump ...
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bioRxiv - Molecular Biology 2019Quote: ... The soluble fraction of the lysate was loaded on a nickel column (HisTrap Hp, GE healthcare). The column was extensively washed with wash buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... IP is carried out with clarified cell lysate pre-cleaned by protein G sepharose (GE Healthcare), then the cell lysate was incubated with anti-LRRC33 or control mouse IgG (2μg/ml ...
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bioRxiv - Genetics 2021Quote: ... Protein lysates were resolved by 12% SDS-PAGE and transferred to PVDF membrane (GE Healthcare AmershamTM) using a Trans-Blot® SD Semi-Dry Transfer Cell (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... 4 °C) and the lysate was loaded onto a StrepTrap HP 5 mL column (GE Healthcare) and purified with the ÄKTA™purifier system (GE Healthcare ...
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bioRxiv - Microbiology 2019Quote: ... lysed and the cell lysate was loaded onto a 1 ml GSTrap HP column (GE Healthcare) before elution using 50 mM Tris pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... The cleared lysate was loaded into a pre-equilibrated HisTrap column (GE Healthcare 1mL HisTrap column) using an ÄKTA Pure Protein Purification System (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... The cleared cell lysates were incubated overnight with glutathione-Sepharose 4B beads (GE Healthcare Life Sciences) in the presence of 5 mM dithiothreitol (DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Affinity purification was carried out by passing the clarified lysate over a GSH column (GE Healthcare) using the ÄKTA Prime Plus system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The filtered cell lysate was loaded onto a 1 ml HisTrap HP Ni2+ column (GE Healthcare) before elution using a gradient of Buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The proteins were purified from the cell lysate using 300 μl GST resin (GS4B, GE Healthcare), and incubated with excess purified wild-type or mutant rDPP9 proteins on ice for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
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bioRxiv - Plant Biology 2022Quote: ... the cell lysate was incubated with Ni-NTA beads (Ni Sepharose 6 Fast flow, GE Healthcare) for 45 mins ...
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bioRxiv - Biochemistry 2022Quote: ... lysate was passed through an immobilized metal affinity chromatography (IMAC) column (GE Healthcare HisTrap™ HP) equilibrated with 5 column volumes (CVs ...
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bioRxiv - Biochemistry 2022Quote: ... the resulting clarified lysate was batch-bound to ~1 mL of settled IgG sepharose (GE Healthcare) equilibrated in lysis buffer and allowed to incubate on a roller for 2 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and applied onto GSTrap FF 5 mL (GE Healthcare, USA) using a buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The clarified cell lysate was first injected onto a 5 ml Ni2+-NTA column (GE Healthcare). The complexes were step-eluted with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysates prepared by sonication and centrifugation were purified on a HisTrap HP column (GE Healthcare). The N-terminal polyhistidine-GST tag was cleaved by incubation with TEV protease at 4 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: Lysates were purified using a 1 mL HisTrap HP Ni-sepharose column (GE Healthcare Life Sciences) connected to an ÄKTA pure protein purification system (Cytiva) ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate containing the protein of interest was loaded onto a 5 mL HisTrap column (GE Healthcare) equilibrated in 200 mM potassium phosphate ...
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bioRxiv - Biophysics 2023Quote: ... clarified lysate obtained from the cells was bound to a HiTrap Chelating HP column (GE Healthcare) in low imidazole buffer (50 mM PIPES ...