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Citations for GE Life Sciences :
401 - 450 of 885 citations for Recombinant Rat Il6st Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The conditioned medium was flowed through a 5 ml His-Trap fast-flow (GE life sciences) column at a flow rate of 4 ml/min using an NGC chromatography system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... RSFPs were isolated from the supernatant with the His SpinTrap kit (GE Healthcare, Chicago, IL, USA) and concentrated with Vivaspin 500 centrifugal concentrators with a molecular weight cut-off of 10,000 kDa (Sartorius ...
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bioRxiv - Biochemistry 2020Quote: ... The excessive imidazole was removed using a desalting column (Hi-Prep™ 26/10, GE Healthcare). The 8 × His-SUMO was cleaved by TEV proteinase and the cleaved fragment was removed through the affinity of the His-tag to a Ni-NTA column ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... and the supernatant was applied to a His-trap Ni-NTA column (5 mL, GE Healthcare) according to the manufacture instruction using an Äktapurifier platform (GE Healthcare) ...
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bioRxiv - Biophysics 2021Quote: ... Lysate was cleared by centrifugation and was subjected to 5 ml His-Trap columns (GE Healthcare). The protein was eluted with a linear imidazole gradient of 15–250 mM in 30 column volumes ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded onto a heparin column (Hi-trap Heparin HP, GE Healthcare Life Sciences) and MatP was eluted with a NaCl gradient (0.3 to 1M ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared via centrifugation and loaded to His Spin Trap spin column (GE Healthcare). The column was sequentially washed with PBC containing 20mM ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysates was subjected to Nickel gravity column using 5 ml His-Trap columns (GE Healthcare), and protein was eluted with 300 mM imidazole in column volumes ...
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bioRxiv - Microbiology 2020Quote: ... His-SUMO-NahA filtered supernatant was purified using a 5 mL HisTrap FF column (GE Healthcare) equipped on an ÄKTA FPLC apparatus (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... His-tag removed protein was further purified by size-exclusion chromatography (S200 16/60; GE Healthcare) in buffer containing 20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the clarified cell-free extracts were loaded onto a His-Trap FF crude column (GE Healthcare) preequilibrated with buffer B containing 0.1% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... 2 mM β-mercaptoethanol) and applied to a 1-ml Hi-Trap Heparin column (GE Healthcare) equilibrated with TGED ...
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bioRxiv - Cell Biology 2019Quote: ... His-SEPT2-mCherry-SEPT6/7-strep was further purified using an AKTA FPLC system (GE Healthcare) with a Superdex 200 10/300 GL (Amersham Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs were further purified using a 1 or 5 ml Hi-trap Q column (GE Healthcare) that was equilibrated in (100 mM NaCl,10 mM KH2PO4 ...
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bioRxiv - Biochemistry 2021Quote: ... an initial IMAC step was performed on a His-Trap column (all columns by GE Healthcare) using buffers A (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: Proteins were purified according to their His6-tag using a His Excel column from GE Healthcare attached to a Äkta Start system ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified by affinity chromatography on a 5 ml His-Trap FF crude column (GE Healthcare), the protein was further purified by gel filtration using a HiLoad Superdex 200 16/600 column (GE Healthcare).
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bioRxiv - Biophysics 2022Quote: The N-terminal 6x His tags were removed from the histones by thrombin protease (GE Healthcare) treatments using 1 unit per 1 mg of protein for 3-5h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified using the His GraviTrap column (GE Healthcare Life Sciences, 11-0033-99) by following the manufacture’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... His-GCN4-FCT was further purified on a HiLoad 10/600 Superdex S200 column (GE Healthcare) in 50mM NaH2PO4-Na2HPO4 ...
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bioRxiv - Cell Biology 2022Quote: ... the elution fractions containing His-ZNF524 were further purified on a HiTrap Heparin HPTM (GE Healthcare). Therefore ...
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bioRxiv - Molecular Biology 2023Quote: ... mv4Int was purified on nickel-nitrilotriacetic acid affinity resin (1ml His-trap HP, GE Healthcare, USA). Column equilibration was performed by injecting 10 column volumes of buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... All Parkin constructs were further purified over Anion exchange column Hi-trap Q HP (GE Healthcare) followed by Hiload 16/600 Superdex 200pg column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... supernatants filtered through 0.45 µm syringe filter and loaded onto His Trap (5 mL, GE Healthcare) equilibrated with 5% HisB buffer (100% HisB ...
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bioRxiv - Biophysics 2024Quote: ... The protein sample was passed through His Trap™ FF crude column (GE Healthcare, Piscataway, NJ). We expected the undigested protein to remain bound to the column ...
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bioRxiv - Biochemistry 2024Quote: ... The free dye was separated from labelled protein by a Hi-TRAP Desalting column (GE Healthcare). The final degree of labelling of FtsZ and ZapD was calculated from the molar absorption coefficients of the protein and the dye ...
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bioRxiv - Genetics 2021Quote: ... anti-rat or anti-rabbit HRP-conjugated antibodies (GE Life Sciences) as appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... and Secondary horseradish peroxidase -linked antibodies: anti-Rat (GE healthcare; NA935), anti-Mouse (GE health-care ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rat horseradish peroxidase (GE Healthcare NA935, 1:2,000). Blots were developed by chemiluminescence using Amersham ECL Prime Detection Reagent (GE Healthcare).
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2020Quote: ... Fc-fused sdAbs were prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE and the Western blot using standard protocols for dimerization ...
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bioRxiv - Microbiology 2021Quote: ... Fc-fused nanobody was prepared with prepacked HiTrap® Protein A HP column (GE Healthcare). The produced Fc-fusion protein was analyzed by SDS-PAGE using standard protocols for dimerization ...
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bioRxiv - Immunology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare) and cleaved using bovine enterokinase (GenScript ...
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bioRxiv - Microbiology 2020Quote: ... The RBD-Fc fusion was purified from filtered supernatant on Protein G Sepharose (GE Healthcare). The protein was cleaved using bovine enterokinase (GenScript ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.
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bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated recombinant NS1 was purified by FPLC using a HisTrap HP Column (GE Healthcare), eluted with a 50–250 mM imidazole gradient ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...