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Citations for GE Life Sciences :
451 - 500 of 1774 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... to a 5 mL His-trap crude FF column (GE Life Sciences). This was washed with Buffer A (30 mM Tris-HCl pH ...
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bioRxiv - Microbiology 2021Quote: ... and loaded on 1 ml His-Trap columns (GE Healthcare, Freiburg, Germany), pre-equilibrated with ten column volumes of each ddH2O and sodium phosphate buffer A (20 mM Na2HPO4 * 2 H2O ...
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bioRxiv - Synthetic Biology 2022Quote: ... The supernatant was then run through an equilibrated His-trap (GE healthcare) in an AKTA start fast protein liquid chromatography system ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was loaded onto a 5mL His-trap column (GE Healthcare) for protein purification and eluted with a linear imidazole (20 mM-200 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... the PCR reactions were loaded on Hi-Trap Streptavidin column (GE Healthcare). By addition of 60 mM NaOH ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were loaded in a 1-ml His-Trap column (GE Healthcare) previously equilibrated with buffer H ...
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bioRxiv - Cancer Biology 2024Quote: ... The supernatant was purified by His TrapTM FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2020Quote: ... PCR amplified fragments of the NP(412-500) or shorter fragments from pCAGGS-NP were cloned into pGEX-6P-2 (GE Healthcare). All sequences of the cloned or mutated DNA regions of the plasmids were validated by Sanger DNA sequencing.
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bioRxiv - Molecular Biology 2020Quote: ... heparin affinity and size-exclusion chromatography using the ÄKTApure chromatography system (GE Healthcare). First ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was loaded onto a cobalt-Talon affinity agarose resin (GE Healthcare). The target protein was eluted with lysis buffer supplemented with 250 mM imidazole and 0.5 mM EDTA ...
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bioRxiv - Molecular Biology 2021Quote: ... The protein was affinity purified with streptavidin sepharose high performance beads (GE Healthcare), washed three times with l × lysis buffer containing 200 mM NaCl and 0.1% TritonX-100 ...
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bioRxiv - Biochemistry 2019Quote: ... the supernatant was bound to a glutathione affinity column (GSTrap FF, GE Healthcare) in buffer A (50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: ... After initial purification using immobilized metal-affinity chromatography (IMAC) (HisTrap FF, GE Healthcare), the crude CopY sample was incubated at 23 °C with a 100:1 mass ratio of recombinant TEV ...
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bioRxiv - Cell Biology 2019Quote: ... Augmin holocomplex was affinity-purified using IgG-Sepharose (GE Healthcare, 17-0969-01) and eluted via cleavage with 100–200 µg of GST-HRV3C protease ...
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bioRxiv - Cell Biology 2019Quote: ... XMAP215 was affinity-purified using a HisTrap HP 5 ml column (GE Healthcare), followed by cation-exchange with a Mono S 10/100 GL column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... followed by a Nickel-ion affinity column (Chelating-Sepharose Fast-Flow, GE Healthcare). The protein was eluted from the column with a gradient of 1 mM to 40 mM histidine ...
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bioRxiv - Biochemistry 2021Quote: ... Lysate supernatants were loaded onto a HiTrap Talon metal affinity column (GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The eluted proteins were concentrated and purified by heparin affinity chromatography (GE Healthcare) using buffer A (30 mM HEPES pH 7.8 ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified by batch purification using TALON affinity resin (GE Healthcare) and treated with HRV-3C protease (Novagen ...
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bioRxiv - Biochemistry 2020Quote: ... Mutant TTR were purified by Ni-affinity chromatography using HisTrap columns (GE Healthcare), followed by gel filtration chromatography using a HiLoad 16/60 Superdex 75 column (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was applied into a 5 ml HisTrap affinity column (GE Healthcare) pre-equilibrated with buffer A (20mM sodium phosphate pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... After purification by NiNta affinity chromatography (Äkta Start, GE Healthcare, Chicago, Illinois, USA) the protein was further purified using a size-exclusion column (Superdex ...
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bioRxiv - Biochemistry 2022Quote: ... The protein was purified with the Ni-NTA Superflow affinity column (GE Healthcare) and Sephacryl S100 column (GE Healthcare ...
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bioRxiv - Biochemistry 2022Quote: ... The affinity-purified protein was then subjected to StrepTrap HP column (GE Healthcare) to remove silkworm-derived proteins and eluted with buffer containing 2.5 mM desthiobiotin (DTB ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was passed through a 5 ml Ni2+ affinity column (GE Healthcare) and eluted with the same lysis buffer containing imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... and lysates were purified by affinity chromatography via AKTA FPLC (GE Life Sciences).
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bioRxiv - Neuroscience 2021Quote: ... The produced vectors were then purified by affinity chromatography (GE Healthcare, Chicago, USA), concentrated to 150 μl by ultrafiltration (Amicon Ultra-4 10K MWCO ...
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bioRxiv - Biochemistry 2021Quote: ... Purification was achieved by affinity chromatography (GSTrap FF 5 mL column (GE Healthcare)) in 25 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: ... PETISCO was subsequently purified using Heparin affinity chromatography (HiTrap Heparin HP, GE Healthcare)) and size-exclusion chromatography using an S200 increase 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... affinity-purified proteins were spotted onto Protran nitrocellulose membranes (0.45 μm, GE Healthcare). After being dried ...
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bioRxiv - Microbiology 2019Quote: ... Cleared lysates were loaded onto a nickel affinity column (HisTrap FF, GE Healthcare) mounted on an Akta chromatography system ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare) and the purity and integrity were checked by SDS–PAGE ...
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bioRxiv - Immunology 2019Quote: ... Abs were purified using affinity chromatography (Protein A Sepharose Fast Flow, GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... (iii) during Ni2+-affinity chromatography the column (HiTrap Chelating HP columns, GE Healthcare) was washed with (20 mM Hepes ...
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bioRxiv - Biochemistry 2020Quote: ... The MRPP1/MRPP2 complex was purified using a nickel affinity column (GE Healthcare) and the His-tag was removed from MRPP1 by by incubation with TEV protease ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded onto a nickel affinity column (HisTrap HP, GE Healthcare) on an AKTA Avant instrument ...
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bioRxiv - Biochemistry 2019Quote: ... The complex was further purified by HiTrap Heparin HP affinity column (GE Healthcare) after diluting eluant ~5-fold with 20 mM sodium phosphate buffer pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... and the soluble lysate was purified over a glutathione affinity column (GE Healthcare). Bound complex was eluted via overnight on-column TEV cleavage to remove the GST-and His-tags ...
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bioRxiv - Biochemistry 2021Quote: ... and applied to a HiTrap heparin HP affinity purification column (GE Life Sciences) equilibrated in 50 mM NaCl ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were purified sequentially by Ni-NTA affinity chromatography (HisTrap FF, GE Healthcare), heparin chromatography (HiTrap Heparin HP ...
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bioRxiv - Cell Biology 2020Quote: ... Following the initial affinity purification with a 5 mL HisTrap column (GE Healthcare), the pooled eluate was incubated with TEV protease at a 1:50 ratio of protease:Sec13/31 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was loaded onto a glutathione-Sepharose-4B affinity column (GE Healthcare) equilibrated with lysis buffer and washed until a stable baseline was obtained ...
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bioRxiv - Biophysics 2022Quote: ... supernatant was passed through Ni-NTA affinity column (5ml, HisTrap column, GE Healthcare), followed by ion exchange Heparin column (HiTrap Heparin HP ...
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bioRxiv - Biophysics 2022Quote: ... NQO1 proteins were purified using immobilized nickel affinity chromatography columns (IMAC, GE Healthcare) as described (12) ...
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bioRxiv - Biophysics 2022Quote: ... which was used for affinity purification with 5 mL NiNTA column (GE Healthcare), which was previously equilibrated in NiA buffer (50 mM Tris pH8 ...
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bioRxiv - Microbiology 2022Quote: ... were loaded onto a 5-ml Ni-affinity column (HisTrap HP, GE healthcare) and washed with buffer B (50 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... the antibodies were further affinity purified using Protein G mag sepharose (GE Healthcare).
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Biochemistry 2023Quote: ... 10% (v/v) glycerol) and loaded onto a heparin affinity column (GE healthcare) and eluted with 100% buffer D (buffer C + 1.5 M NaCl) ...