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Citations for GE Life Sciences :
151 - 200 of 990 citations for Recombinant Mouse Tigit Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare) via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... Recombinant antibodies were purified from supernatants using protein-G beads (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange on a HiPrepTM 26/10 desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant protein was then isolated using Ni-NTA resin (GE healthcare) and eluted with a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were purified on a HiTrap SP HP column (GE Healthcare) and subsequently on a HiTrap Q HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant proteins were first purified using a Ni-NTA column (GE HealthCare) and the His-GST tag was removed by cleavage with 3C-PreScision protease and subsequent incubation with Ni2+ beads to remove the uncleaved protein and the tag from the solution ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2020Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL western blotting detection reagent (GE Healthcare). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
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bioRxiv - Plant Biology 2021Quote: ... L7TIR and MBP tagged L7TIR were subjected to Superose 6 10/300 gel filtration column (GE Healthcare). Fractions eluted at void volume were collected ...
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bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
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bioRxiv - Microbiology 2020Quote: ... S1 -Fc proteins were purified using Protein A-Sepharose beads (GE Healthcare Life sciences) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were first purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant tau proteins were immobilized on the CM5 sensor chip (Biacore, GE Healthcare) via amine coupling ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... The recombinant CoxbHtrA protein was purified using an ÄKTA avant system (GE Healthcare) equipped with a 5 mL StrepTrap HP column (GE Healthcare) ...
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bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
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bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
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bioRxiv - Biophysics 2019Quote: ... The hexa-histidine-SUMO-tagged fusion protein was purified using a nickel charged column (HisTrap HP, GE healthcare). After elution with a 750mM imidazole containing buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Molecular Biology 2021Quote: ... and GST-tagged TopBP1 protein was purified on Glutathione Sepharose™ 4B (GE healthcare; catalog no. 17-0756), according to the batch purification method described in the manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tagged phytochrome samples were first purified with NiNTA affinity purification using HisTrap™ columns (GE Healthcare), followed by size-exclusion chromatography (HiLoad™ 26/600 Superdex™ 200 pg ...
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bioRxiv - Microbiology 2021Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL Western blotting detection reagent (GE Healthcare, #GERPN2209). In a parallel gel ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were clarified by centrifugation and GST-tagged protein were isolated on glutathione sepharose 4B resin (GE Healthcare) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell-free synthesized N11-tagged hTERT_191-306 protein was purified using an AKTA 10S system (GE Healthcare) with a HisTrap columns (GE Healthcare) ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Biophysics 2021Quote: ... Cell pellets were sonicated and His-tagged proteins were purified using affinity chromatography (HisGraviTrap column from GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...