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Citations for GE Life Sciences :
551 - 600 of 2792 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... placed in 5% milk PBST plus 1:2500 mouse specific HRP antibodies (GE Healthcare) at room temperature for 4 hours ...
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bioRxiv - Neuroscience 2021Quote: ... anti-mouse or anti-rat secondary antibodies (1:4000 in blocking buffer, GE Healthcare) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with biotinylated sheep anti-mouse (1:200, RPN1001V, GE Healthcare) followed by peroxidase labeled streptavidin (1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... and HRP-linked Sheep Anti-Mouse IgG (1:2000; GE Healthcare Life Sciences, NAG31V). For the analysis of p65 NFκB localisation and phosphorylation ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Genetics 2019Quote: ... or HRP-conjugated anti-mouse IgG (GE Healthcare NA931V, Lot 9648752; 1:10000 dilution). Signals were produced by enhanced chemiluminescence (ECL ...
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bioRxiv - Cancer Biology 2020Quote: ... horseradish peroxidase (HRP)-conjugated anti-mouse antibody (NA931, GE Healthcare Life Sciences, 1:10,000) or HRP-conjugated anti-rabbit antibody (NA934V ...
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bioRxiv - Microbiology 2021Quote: ... membrane was finally incubated with HRP-conjugated anti-mouse IgG (1:5000; GE Healthcare) overnight at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The soluble fraction was found to contain more AβM(E22G)-mCherry than the insoluble fraction (Supplementary Figure 5. ...
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bioRxiv - Cell Biology 2021Quote: ... followed by anti-rabbit and mouse antibodies coupled to HRP (1:2000, GE Healthcare).
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bioRxiv - Cancer Biology 2021Quote: ... After 1 hour incubation at RT with the respective anti-mouse (NA931, GE Healthcare) or anti-rabbit (#7074 ...
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bioRxiv - Biophysics 2020Quote: ... and a secondary anti-mouse IgG HRP-conjugated antibody (1:1000, #NA931, GE Healthcare). The membrane was dried and reprobed with an anti-mCherry antibody (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Primary antibody labelling was done for 1 hour using mouse anti-BrdU (GE Healthcare) and rat anti-HA (Roche ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Secondary antibodies used: anti-mouse-IgG-Horseradish Peroxidase (GE Healthcare, Buckinghamshire, UK, 1:4000).
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated with horseradish peroxidase (HRP)‐linked anti‐mouse (1:5000; GE Healthcare) or HRP‐linked anti‐ rabbit (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... ECL Mouse IgG and ECL Rat IgG (1:5000, NA934, NA931, NA935, GE Healthcare) and immunoblots were imaged using NuGlow ECL (Alpha Diagnostics) ...
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bioRxiv - Bioengineering 2020Quote: ... for HRP-conjugated antibodies: donkey monoclonal anti-mouse (1:10000) and donkey monoclonal anti-rabbit antibody (1:10000, GE Healthcare) diluted in 2% BSA-TBST ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in TBS before a 1 hour incubation with a 1:5,000 dilution of sheep anti-mouse IgG horseradish perodixase secondary antibody (GE Healthcare) in 5% milk-TBS ...
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bioRxiv - Neuroscience 2024Quote: ... followed by 1 h incubation with the respective secondary antibody at RT (HRP-conjugated anti-rabbit, 1/4.000, #NA9340, GE Healthcare; HRP-conjugated anti-mouse, 1/3.000, #NA9310, GE Healthcare). An immune signal was detected using Clarity Western ECL substrate (#1705061 ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion EtAHS A protein was purified from cell lysates with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The fusion protein was cleaved by incubation with thrombin protease (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing tagged-SbtB7001 were pooled and transferred into binding buffer using a HiPrep 26/10 Desalting column (GE Healthcare). The His6–Ub tags were removed using a ubiquitin carboxyl-terminal hydrolase 2 catalytic core domain (USP2cc):SbtB7001 molar ratio of 1:25 at 4 °C overnight as previously described [14] ...
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bioRxiv - Cell Biology 2020Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2019Quote: ... antigen affinity columns were prepared by conjugating purified antigens (hexa-histidine-tagged PICH C-terminus fragment or GST-tagged TopoIIα C-terminus fragment) to the NHS-Sepharose resin following manufacture’s protocol (GE healthcare). The rabbit antisera were subjected to affinity purification using antigen affinity columns ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared lysate was incubated with either Glutathione Sepharose® 4B (for GST-tagged proteins, GE Healthcare, #17-0756-01) or Protino Ni-TED resin (for SUMO-tagged proteins ...
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bioRxiv - Physiology 2020Quote: ... YFP-tagged proteins were visualized by scanning the wet PAGE gels with a fluorescence scanner (Typhoon, GE Healthcare, München, Germany). Individual bands were quantified with ImageJ (53).
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bioRxiv - Microbiology 2021Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer (50 mM NaH2PO4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Capture of biotin-tagged nascent DNA was performed by incubating samples with streptavidin sepharose high performance (GE Healthcare Life Sciences) agarose beads for 16 h ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant containing the 6xHis-tagged DMTases was stirred with 6 mL of NiCl2-charged chelating Sepharose beads (GE Healthcare) for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Fractions containing purified His6-tagged proteins were combined and loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His6-tagged protein was further purified using a HiLoad 16/60 Superdex 200 prep-grade size exclusion column (GE Healthcare) in 50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system (GE Healthcare). First ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare). Endotoxin was removed by Pierce High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Biophysics 2019Quote: ... All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg, GE healthcare) pre-equilibrated with the buffer containing 20mM HEPES pH 7.5 and 150 mM NaCl (Supplementary Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... RAS:Affimer immunoprecipitation was as for RAS:RAF immunoprecipitation except His Mag Sepharose™ Ni2+-NTA beads (GE healthcare®) were used and incubated with 20 µg of Affimer K3 for 1 h with agitation ...
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bioRxiv - Biochemistry 2020Quote: ... Ni Sepharose High Performance (His Trap HP) and Superose 6 10/300 column were purchased from GE Healthcare.
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bioRxiv - Biochemistry 2021Quote: ... followed by gel filtration chromatography using Hi-Load 16/600 superdex 200 pg and 75 pg (GE Healthcare) on AKTA pure and AKTA prime systems ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Untagged His-GmPUB13 U-box and Avr1d were loaded on a superdex 200 gel-filtration column (GE Healthcare) and eluted in superdex buffer (20 mM Tris [pH 8.0] ...
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bioRxiv - Biophysics 2021Quote: ... and the final product was obtained by running the solution through an 5 mL Hi-Trap (GE Healthcare) desalting column (Figure S1).
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His- Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Biophysics 2022Quote: ... The clarified supernatant was then passed over a Ni-NTA His-Trap FF 5 ml column (GE Healthcare) which was pre-equilibrated with loading buffer composed of 25 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then applied onto a Hi Trap affinity (5 ml) (Ni2+) column (GE Healthcare Life Sciences) The column was washed with 10 column volumes of buffer A ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was incubated with His Mag Sepharose beads (equilibrated with lysis buffer according to manufacturer’s instructions; GE Healthcare) for 30 min at 4 °C rotating end-over-end ...