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Citations for GE Life Sciences :
351 - 400 of 990 citations for Recombinant Mouse Cd83 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... and 6xHis-SMT3/SUMO tagged IMPDH2 was initially purified by Ni affinity chromatography using either a HisTrap FF column (GE Healthcare Life Sciences) on an Äkta Start chromatography system or a handpacked HisPur™ Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
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bioRxiv - Immunology 2021Quote: Purified recombinant antibodies were assessed by SDS-PAGE under nonreducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were further purified by anion-exchange chromatography on a Resource Q column (GE Healthcare, United States) that was pre-equilibrated with buffer A (20 mM Tris–HCl ...
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bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
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bioRxiv - Immunology 2019Quote: ... The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6, 10/300 GL;GE Healthcare). The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos ...
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bioRxiv - Immunology 2020Quote: ... Recombinant proteins were purified directly from the filtered culture supernatants using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Immunology 2023Quote: ... Recombinant HA molecules were purified from these culture supernatants by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with imidazole gradient ...
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bioRxiv - Microbiology 2019Quote: ... Anti-mouse (GE Healthcare) or rabbit-HRP (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... SNAP-tagged proteins were filtered on a Superdex 200 10/300 GL or Superose 6 Increase 10/300 GL column (GE Healthcare, Little Chalfont, UK) and then labeled with SNAP-surface-549 (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... Human IgG1 Fc-containing proteins were purified using Protein A Sepharose CL-4B (GE Healthcare, Cat. No. 17-0780-01), eluted with 0.1 M citric acid at pH 4.5 and neutralized with 1 M Tris-HCl at pH 9.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Over 9500 Response Units of the recombinant HDP protein were immobilized on S-Series CM5 sensor chip (GE Healthcare) using 10mM sodium acetate pH4.0 solution (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: Molecular masses of recombinant GgNAD-MEs were evaluated by gel-filtration chromatography on an ÄKTA purifier system (GE Healthcare) using a Biosep-SEC-S3000 column (Phenomenex) ...
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bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: Filtered soluble recombinant wild-type RBPMS-A and mutants were purified on an AKTA protein purification system (GE Healthcare;). Recombinant protein was first purified on HisTrap HP column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing recombinant protein were combined and purified by gel filtration on a S200 26/60 column (GE Healthcare) equilibrated with standard TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... normal mouse IgG (GE Healthcare)
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse IgG (GE Healthcare) or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... anti-mouse IgG (GE Healthcare, distributed by VWR in Ismaning ...
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bioRxiv - Developmental Biology 2020Quote: ... or anti-mouse (GE Healthcare) antibodies linked with horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2020Quote: ... CR2-Fc and FH19-20 were prepared in 10 mM sodium acetate pH 5 and immobilised at 300 RU (CR2-Fc: 20 µM, FH19-20: 240 µM) to different flow cells of CM5 chips (GE Healthcare) using standard amine coupling involving preparation of the dextran matrix with 100 mM N-hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2020Quote: ... NP_068576.1) with C-terminal Fc tag was expressed in HEK 293 cells were purified by protein A sepharose beads (GE Healthcare).
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bioRxiv - Biophysics 2020Quote: ... The Anti-hIgG Fc (AHC) capture biosensors were purchased from ForteBio (California, USA) and the protein A coated chips were purchased from GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... The Fab fragments are then separated from non-digested IgG and Fc fragments by a 1 hr incubation with rProtein A Sepharose Fast Flow (GE Healthcare). Post incubation ...
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bioRxiv - Microbiology 2020Quote: ... the VHH-72-Fc protein in the cleared cell culture medium was captured on a 5 mL MabSelect SuRe column (GE Healthcare), eluted with a McIlvaine buffer pH 3 ...
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bioRxiv - Cell Biology 2020Quote: ... a total of 1×104 RAW 264.7 (ATCC) cells were seeded per well on 24-well plates in 500 μl αMEM containing 5% FCS (Hyclone, GE Healthcare), 1% penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and ACE2(HH:NN)-Fc constructs were captured on flow cell 2 of a Series S Protein A sensor chip (GE Healthcare – 29127555) to a density of 500 RU using a Biacore 8K instrument (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... We separated pFab and Fc from undigested IgG using size exclusion chromatography with a Superdex 200 Increase 10/300 column (GE Healthcare) and concentrated the pFab/Fc.
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bioRxiv - Molecular Biology 2023Quote: Antibodies were expressed by transient transfection in Expi-CHO cells as murine IgG2a Fc domain conjugates and purified using the HiTrap MabSelect SuRe (GE Healthcare) affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... The antibodies were captured on a CM5 chip immobilized with anti-human IgG Fc using a Human Antibody Capture Kit (GE Healthcare), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Germany) were grown in RPMI 1640 medium containing 10% heat-inactivated fetal calf serum (FCS, GE Healthcare, Freiburg, Germany or Merck) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... hTIGIT-ECD in fusion with a C-terminal Fc tag (Acro Biosystems, Cat. No. TIT-H5254) was immobilized on CM5 sensor chips (GE Healthcare) in 10 mM sodium acetate ...
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bioRxiv - Cell Biology 2020Quote: 80 μg of the purified recombinant DNC-1 fragments were loaded on a Superose 6 10/300 column (GE Healthcare) equilibrated with storage buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinants showing intense hybridization signal selected for the plasmid extraction using the IllustraTM plasmidPrep Mini Spin Kit (GE Healthcare). The inserts released by the EcoRI (Invitrogen ...