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Citations for GE Life Sciences :
401 - 450 of 4576 citations for Recombinant Mouse Cd40lg Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was further purified with size exclusion chromatography to remove any protein aggregates applied to a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...
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bioRxiv - Biochemistry 2022Quote: ... The fractions containing the protein of interest were pooled and concentrated to ∼3 mg/mL using a 100kDa MWCO Vivaspin protein concentrator (GE Healthcare). The concentrated protein was subjected to size exclusion chromatography on Superose 6 increase 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2019Quote: ... Proteins were purified by either Protein A affinity or by using ion exchange chromatography (MonoQ 10/100 GL column, GE Healthcare) with a 0 mM–1 M NaCl gradient ...
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bioRxiv - Immunology 2020Quote: ... Recombinant IgG antibodies were purified from cell-free supernatants by affinity purification using Protein G or Protein A sepharose beads (GE Healthcare). Purified proteins were dialyzed in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... and a two-step fast protein liquid chromatography (FPLC) purification protocol using an ÄKTA Protein Purification System (GE Healthcare Life Sciences) at 8 °C was then followed to obtain the recombinant protein ...
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bioRxiv - Immunology 2022Quote: ... The culture was harvested after seven days and the supernatant purified into PBS by Protein G affinity using a HiTrap Protein G HP affinity column (GE Healthcare), eluted with 0.1M acetic acid (VWR international ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Biophysics 2019Quote: ... This mixture was then filtered through a 0.2 μM filter and concentrated on a 10 kDa (for EC1-5/EC3-5 proteins) or a 100 kDa (for full length proteins) MWCO column (Vivaspin, GE Healthcare). The protein was buffer exchanged 2-3x using either 7 kDa MWCO Zeba Columns for EC1-5/EC3-5 proteins or 40 kDa MWCO Zeba Columns for full length proteins (ThermoFisher).
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bioRxiv - Neuroscience 2019Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at - 80°C until use ...
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bioRxiv - Biophysics 2019Quote: ... After dilution of the protein sample to a DTT concentration of 1 mM the reduced protein were immobilized on a Ni2+-Sepharose resin (GE Healthcare) and washed with ten column volumes of buffer A (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
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bioRxiv - Immunology 2021Quote: ... The ACE2-Ig and RBD-Ig fusion proteins secreted to the medium were purified on HiTrap Protein G High Performance column (Cat#GE17-0405-01, GE Healthcare). Control-Ig was one of the following fusion proteins ...
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at 16,000xg for 10 min and the clarified lysates were pre-adsorbed to Protein A or Protein G beads (GE Healthcare Bioscience) for 2 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the clarified supernatant containing the NLRP1NACHT-LRR protein with maltose-binding protein (MBP) tag was purified on Hisprep IMAC column (GE Healthcare) and eluted with elution buffer contained 50 mM Tris-HCl at pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Elution fractions containing the untagged protein were pooled and the homogenous protein was finally isolated by size-exclusion chromatography onto a Superdex 75 column (GE Healthcare) equilibrated in 20 mM Hepes/NaOH ...
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bioRxiv - Neuroscience 2022Quote: ... Equal volumes of cell lysates containing ∼1000 μg protein were precleared with 10 μl Protein A Sepharose (GE Healthcare, Freiburg, Germany) for 1 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... lysates were incubated with the primary antibody at 4°C for 2 h or overnight and then incubated with protein G- or protein A-Sepharose (GE Healthcare) for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein quantification was performed using BCA and proteins was separated on SDS-PAGE followed by transfer to PVDF membrane (GE Healthcare). Next ...
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bioRxiv - Neuroscience 2023Quote: Proteins from the samples were purified by a modified trichloroacetic acid protein precipitation procedure (Clean-Up Kit; GE Healthcare, Munich, Germany), and gel-assisted proteolysis was carried out ...
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bioRxiv - Immunology 2024Quote: ... The antibody was purified from culture supernatant by protein G affinity chromatography using the ÄKTA Pure system with a HiTrap Protein G column (GE Healthcare).
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bioRxiv - Neuroscience 2021Quote: ... The secondary antibody was anti-mouse HRP (horseradish peroxidase)-linked (NA931, Amersham ECL Mouse IgG, HRP, GE Healthcare Life Science) diluted 1:10000 in 5% dry milk in PBS ...
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bioRxiv - Biophysics 2019Quote: ... chip was prepared by amine coupling of anti-mouse IgG antibodies at pH 5.0 (Mouse antibody capture kit, GE Healthcare) according to the protocol described by manufacturer ...
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bioRxiv - Cell Biology 2023Quote: ... in a final volume of 260 µl and injected into the CodeLink Uniset Mouse I Bioarrays containing 10,000 mouse genes probes (GE Healthcare). Arrays were hybridized overnight at 37° C at 300 rpm (8 g) ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-mouse IgG-HRP (GE Healthcare, UK). HRP-labeled proteins were detected with Amersham ECL Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... anti-mouse IgG (GE Healthcare, Chicago, IL, USA) or anti-rabbit IgG (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-BrdU (1:1; GE Healthcare, RPN202); mouse anti-PCNA (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... sheep anti-mouse (NA9310V, GE Healthcare Life Sciences), donkey anti-sheep (713-035-147 ...
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bioRxiv - Molecular Biology 2020Quote: ... or anti-mouse secondary antibody (GE Healthcare, NA931V). Proteins were visualized using Pico Plus chemiluminescence substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... anti-mouse IgG-HRP (Amersham, GE Healthcare, UK) was used as secondary antibody and incubated for 1 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... anti-mouse secondary antibody (1:4000, GE Healthcare) or alpaca anti-chicken (1:4000 ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies were anti-mouse-HRP (GE Healthcare) or anti-rabbit-HRP (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... goat anti-mouse IgG-HRP (NA931, GE Healthcare), and using an enhanced chemiluminescent visualization system (Cat ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rat and anti-mouse antibodies (GE Healthcare) used at a dilution of 1:5,000 ...
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bioRxiv - Systems Biology 2021Quote: ... secondary mAB anti-mouse (sheep, GE Healthcare, LNA931V), secondary mAB anti-rabbit (donkey ...
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bioRxiv - Microbiology 2020Quote: ... antibody with HRP-conjugated anti-mouse (GE Healthcare) or HRP-conjugated anti-rabbit (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-mouse (1:10,000, GE Healthcare, Cat. #NA931V) anti-Rat (1 ...
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bioRxiv - Cell Biology 2020Quote: ... and anti-mouse IgG-HRP (GE Healthcare, NA931V), both at 1:3000 dilutions.
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bioRxiv - Cancer Biology 2019Quote: ... goat anti-mouse (1:10,000; GE Healthcare NA931).
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bioRxiv - Cancer Biology 2019Quote: ... or anti-mouse-HRP (1:2000, GE Healthcare). Blots were imaged using a Syngene G-Box system ...
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bioRxiv - Physiology 2021Quote: ... or anti-mouse IgG (GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse-anti-His6 (1:1000, GE Healthcare) and monoclonal rabbit-anti-pT72-Rab8a (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... and ECL sheep anti-mouse (GE Healthcare #NA931V).