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Citations for GE Life Sciences :
501 - 550 of 919 citations for Recombinant Human dipeptidyl peptidase 4 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Biophysics 2021Quote: ... 4°C for 30 minutes and then loaded onto a 5mL NiNTA affinity column (HisTrap HP, GE Healthcare) pre-equilibrated with the lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... 20 µl of a Protein A sepharose (nProtein A Sepharose 4 fast flow, GE Healthcare, 17-5280-01) suspension was added and incubated for 1 hour at 4°C on a rotator ...
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bioRxiv - Cell Biology 2020Quote: Sheared chromatin was pre-cleared with 50 µl protein A Sepharose 4 Fast Flow beads (GE Healthcare, Germany) per 500 µg chromatin for 2h ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C) the sample was applied onto a HiLoad Superdex 200 (16/60) column (GE Healthcare, now Cytiva). Separation was performed at a flow rate of 0.8 mL/min in SEC buffer (50 mM Hepes pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was maintained at 4°C for protein purification with a prepared HisTrap HP column (GE Healthcare). Purified protein samples were concentrated using an Amicon 4 centrifugal filtration tube centrifuged for 5 min at 3600 rcf (4 °C) ...
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bioRxiv - Cancer Biology 2020Quote: ... The triarylmethyl EPR oxygen tracer OX063 (methyl-tris[8-carboxy-2,2,6,6-tetrakis[2-hydroxyethyl]benzo[1,2-d:4,5-d0]-bis[1,3]dithiol-4-yl]-trisodium salt) was obtained from GE Healthcare.
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bioRxiv - Cell Biology 2021Quote: ... the lysates were incubated 16 hours at 4°C with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01). Next ...
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bioRxiv - Immunology 2022Quote: ... 4°C) and mononuclear cells isolated by density gradient centrifugation on a discontinuous 35%/70% Percoll (GE Healthcare) gradient (2000 rpm [931 g] ...
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bioRxiv - Biochemistry 2021Quote: ... 4 °C) and the supernatant applied to a GSTrap 5 ml FF column affinity purification column (GE Healthcare) at 4 °C equilibrated with Tris-Cas-A buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Images (4 fields/well at 20x magnification) were captured through an INCell Analyzer 6000 HCA system (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: All chromatography steps were performed at 4°C using ÄKTAxpress or ÄKTA pure protein purification systems (GE Healthcare). Firstly ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... for isoelectric focusing of samples loaded on 7 cm pH 4-7 Immobiline DryStrips (GE Healthcare, Uppsala, Sweden). We rehydrated each strip for 48 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... Lysates were precleared using 20 μL protein G Sepharose 4 fast flow (1:1 in PBS, GE Healthcare) for 30 minutes at 4°C on a shaker ...
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bioRxiv - Biochemistry 2019Quote: Removal of the His6-tag (Rab8a) was performed by overnight incubation at 4 °C with thrombin (GE Healthcare), followed by a second Ni2+-agarose column ...
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bioRxiv - Immunology 2020Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was applied and circulated on a Glutathione Sepharose 4 Fast Flow 16/10 column (GE Healthcare) for 16 h ...
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bioRxiv - Plant Biology 2022Quote: ... 4°C for 30min and used for the purification of HIS-AgLTP24 using His GravityTrap columns (GE Healthcare) with modified binding (100mM Tris ...
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bioRxiv - Immunology 2021Quote: ... After 6 days the supernatant was harvested and Protein G Sepharose® 4 Fast Flow beads (GE Healthcare) were used for antibody purification.
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bioRxiv - Immunology 2022Quote: The total IgG was purified from murine serum using Protein G Sepharose 4 Fast Flow columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... followed by size-exclusion chromatography at 4 °C on a HiLoad Superdex 200 pg (GE Healthcare Life Sciences) column pre-equilibrated in the same buffer ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Plant Biology 2022Quote: ... Immunoprecipitation was performed by incubating sheared chromatin with Protein G Sepharose™ 4 Fast Flow beads (GE Healthcare) and antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... incubated overnight at 4°C and subjected to size exclusion chromatography using Superdex 200 16/60 (GE Healthcare) in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... and stirred overnight (O/N) at 4°C with 4.5 g CM Sephadex C-25 beads (GE Healthcare) preequilibrated for 3 h with 100 mL of buffer A ...
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bioRxiv - Microbiology 2023Quote: ... The immunocomplex was captured by using protein G Sepharose 4 fast flow beads (17-0618-01/ GE Healthcare). The immunoprecipitated complex was washed two times with IP lysis buffer and one time with mili-Q ...
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bioRxiv - Biochemistry 2023Quote: ... the binding affinity of fragments 1 – 4 and SlpB were evaluated with Biacore X100 (GE healthcare, Uppsala, Sweeden) with sensor chip protein G (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... then 2-D gel samples were collected by scraping cells in 250 ul of 2-D rehydration buffer (4% CHAPS, 8 M urea, 0.5% IPG Buffer (pH 3.0-10.0; GE Healthcare), 0.002% Bromophenol Blue ...
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bioRxiv - Biochemistry 2019Quote: The interaction of the recombinant heterodimeric (‘monomer’) and homodimeric (‘dimer’) human G6b-B extracellular domain with different ligands was quantified using a BIAcoreTM 8K instrument (GE Healthcare, Little Chalfont, UK). Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... or monocytes were isolated from buffy coat of healthy human donors (Biological Specialty Corp) by density centrifugation in Ficoll®-Paque PLUS (GE Healthcare Life Sciences). Monocytes were enriched either through positive selection using CD14 MicroBeads (Miltenyi Biotech ...
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bioRxiv - Cell Biology 2019Quote: ... Lysate was cleared with centrifugation and bound to Glutathione sepharose 4 Fast Flow beads (GE Healthcare, #17-5132-02). Beads were washed several times with lysis buffer and protein was eluted from beads with 10 mM reduced glutathione in lysis buffer ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Cell Biology 2021Quote: ... The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences) for 3-4 hours on a roller ...
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bioRxiv - Microbiology 2021Quote: ... as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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bioRxiv - Cancer Biology 2020Quote: ... All protein lysates were run on NuPage 4–12% gradient gels (LifeTechnology) and transferred to PVDF membrane (GE Healthcare). Membranes were blocked in 5% milk in TBST and stained with primary antibody overnight at +4°C ...
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bioRxiv - Microbiology 2022Quote: ... as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C (W6/32) and rotated with 30 μl pre-equilibrated Protein G Sepharose 4 Fast Flow resin (GE healthcare) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... overnight at 4°C then purified over a Sephadex 200 Increase 10/300 (GE Healthcare Life Sciences, Pittsburgh, PA) size exclusion column in Buffer A with 0.02% DDM.
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bioRxiv - Biochemistry 2021Quote: ... The solution was added to 250 μL (bed volume) Protein G Sepharose™ 4 Fast Flow resin (GE Healthcare) prewashed with DPBS ...
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bioRxiv - Microbiology 2022Quote: ... and for HLA-I IP 25 μl Protein G Sepharose® 4 Fast Flow (GE Healthcare, GE17-0618-01) were employed with the HC10 antibody for HLA-IP overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IgA antibodies and Fab were purified by affinity chromatography using Protein G Sepharose® 4 Fast Flow (GE Healthcare), peptide M-coupled agarose beads (Invivogen ...
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bioRxiv - Cell Biology 2022Quote: ... The concentrated CM was loaded onto a column packed with Sepharose 4 Fast Flow resin (GE Healthcare, IL, USA). Eluates were measured at 280 nm absorbance to monitor the protein content of each fraction ...
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bioRxiv - Biochemistry 2019Quote: RAD51 purification was carried out by chromatography at 4°C using the AKTA Pure Protein Purification System (GE Healthcare). For WT RAD51 ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified GST and GSTPib1ΔFYVE (100 pmol) were immobilised on glutathione Sepharose 4 Fast Flow (25 μl slurry, GE Healthcare). Beads were pre-treated with 1 mg/ml bovine serum albumin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... pH 4) was covalently immobilized on a CM5 sensor chip using an Amine coupling kit (GE Healthcare Life Sciences). Binding analyses were performed in HBS-P buffer (0.01 M HEPES ...
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bioRxiv - Microbiology 2020Quote: ... 2 mM EDTA) for 90 minutes at 4° Celsius and then scanned and examined by Typhoon trio+ (GE Healthcare) scanner using Cy5 channel.
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bioRxiv - Molecular Biology 2019Quote: ... 5% CO2 and maintained in a 4:1 ratio of DMEM (Dulbecco’s modified Eagle’s medium, GE Healthcare, Logan, UT) to Medium 199 containing 10% calf serum (HyClone ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 1h at 4°C followed by incubation with protein A-sepharose beads (GE Healthcare #GE17-0780-01) for 2h at 4°C ...