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Citations for GE Life Sciences :
251 - 300 of 4730 citations for Recombinant Human S100 Calcium Binding Protein A9 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: Approximately 5 μg of GST or GST-tagged protein fragments were incubated in Glutathione-Sepharose beads (GE Healthcare) in GST-lysis buffer at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Strep-tagged proteins were affinity-purified on an ÄKTA Purifier using 5 ml StrepTrap HP columns (GE Healthcare) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Elution fractions containing Strep-tagged proteins were loaded onto a HiLoad 16/600 Superdex 75 pg (GE Healthcare) gel filtration column that was equilibrated with buffer 1 ...
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bioRxiv - Microbiology 2021Quote: ... Expression of the recombinant proteins was performed using the ExpiCHO Expression system (Thermoscientific) following purification using HiTrap Protein-A column (GE Healthcare). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Cell Biology 2020Quote: XMAP215-GFP-7xHis was purified using His-affinity (His-Trap, GE Healthcare) by binding in buffer (50mM NaPO4 ...
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bioRxiv - Biochemistry 2020Quote: ... and the His-tag of Rab8a (anti-His-Antibody, GE Healthcare, mouse). Both were diluted (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare). Endotoxin was removed by Pierce High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Purified recombinant N proteins were loaded onto a Superdex 200 16/30 column (GE Healthcare) and eluted in 20 mM Tris/HCl pH 8.5 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Cleaved recombinant protein was then purified on a MonoQ 5/50 GL column (GE Healthcare) using Buffer QA (described above + 1 mM DTT ...
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bioRxiv - Immunology 2023Quote: ... the recombinant monoclonal antibodies were purified from the supernatant with protein G beads (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: ... The recombinant proteins were batch purified on Nickel-Sepharose beads 6 Fast Flow (GE Healthcare) and eluted with 6xHis-TEV protease ...
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bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences; Table S4).
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed protein was loaded onto two His-Trap HP 1 mL columns (GE Healthcare, Chicago, IL) mounted in series and equilibrated with Buffer B ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were collected and lysed and protein was purified with His-Trap chelating column (GE Healthcare). The purified recombinant proteins were analysed by Coomassie-stained SDS–polyacrylamide gel electrophoresis (PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified by immobilized metal affinity chromatography using the His Trap Kit from GE Healthcare Life Science ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting protein fraction was further purified on a 5 ml Hi-Trap HP SP (GE Healthcare) cation exchange column using an Akta Pure FPLC ...
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bioRxiv - Neuroscience 2020Quote: ... The protein was purified using gel filtration (Superdex75 or 200 Hi-load column, GE Healthcare, Chicago, IL). The peak fractions were pooled and concentrated using filters of appropriate cut-offs (EMD Millipore ...
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bioRxiv - Microbiology 2020Quote: The heterologous proteins containing His-tags were purified via 1 ml Ni-NTA HisTrap columns (GE Healthcare). The cells were lysed in 50 ml lysis buffer containing 50 mM Na-phosphate buffer pH 8 ...
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bioRxiv - Bioengineering 2023Quote: ... The target protein was purified by loading the supernatant onto a prepacked His SpinTrap column (GE Healthcare) and eluting with a buffer containing 20 mM sodium phosphate ...
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bioRxiv - Biophysics 2024Quote: ... the protein sample was passed through a Hi-Trap Q-Sepharose FF crude (GE Healthcare, Piscataway, NJ) column ...
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bioRxiv - Biochemistry 2020Quote: ... MLL1-PHD3 and 6x-His-FL-Cyp33) or Superdex G200 column (His-MBP-FL-Cyp33 and His-SUMO-FL-Cyp33) (both columns from GE Healthcare). After elution ...
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bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
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bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
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bioRxiv - Immunology 2022Quote: ... the supernatant was diluted to a total protein concentration of 100 μg/ml in binding buffer (GE Healthcare). Following purification ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatants of the His6-tagged proteins obtained were incubated with 500μL Ni Sepharose (GE Healthcare 17-5318-06) and then washed twice with buffer A ...
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bioRxiv - Biophysics 2021Quote: ... The 6xHis-tagged protein samples were loaded onto pre-equilibrated Ni2+ affinity resin (HisTrap HP 5 ml, GE Healthcare). The column was sequentially washed with HSU buffer containing 0.5% triton-X ...
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bioRxiv - Biochemistry 2022Quote: ... The histidine-tagged cleaved proteins where then loaded onto a 26/60 Superdex 200 size-exclusion column (GE Healthcare) equilibrated with buffer C ...
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bioRxiv - Biophysics 2019Quote: ... Clarified cell lysate containing His6-tagged protein was loaded onto a Ni–Sepharose affinity column (HisTrap HP; GE Healthcare). The column was washed with wash buffer (5% glycerol ...
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bioRxiv - Cancer Biology 2023Quote: The purified GST-tagged fusion protein WDR522-334 (100 μg) was bound to Glutathione Sepharose 4B (GE Healthcare, 28952360) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare) for 2 hours at 4°C in a rotating wheel ...
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bioRxiv - Microbiology 2024Quote: ... the hexa-histidine tagged H-Rrm4-M protein was purified using Nickel-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta primeplus FPLC system (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein was purified using a 1 ml HisTrap FF column (GE Healthcare, Amersham, United Kingdom) with imidazole gradient elution ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant proteins were purified by Ni2+-affinity chromatography using Histrap HP FPLC columns (GE Healthcare) and a size-exclusion chromatography using a HiLoad 16/60 200 PG column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Recombinant IgG mAbs were purified using HiTrap Protein A columns (Cytiva/GE Healthcare Life Sciences, 17040303).
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were purified from the filtrated supernatants using a HisTrap excel column (GE Healthcare). The purified proteins were biotinylated in vitro using the BirA enzyme in the presence of 0.5 mM Biotin and 10 mM ATP ...
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bioRxiv - Neuroscience 2022Quote: ... recombinant proteins were then eluted from a HiLoad 16/600 Superdex 200 pg column (GE Healthcare) equilibrated with 20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Immunology 2023Quote: ... Recombinant IgG was harvested and purified after 6 days with protein G sepharose beads (GE Healthcare), dialyzed in PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Recombinant proteins were then purified by 1 mL Ni Sepharose (17-5318-01, GE Healthcare, USA). Columns were sequentially washed with 20 mL buffer 1 and 10 mL buffer 2 (in mM ...
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bioRxiv - Biophysics 2019Quote: ... All the purified proteins were further polished using gel filtration column (Hi-load 16/600 Superdex200pg, GE healthcare) pre-equilibrated with the buffer containing 20mM HEPES pH 7.5 and 150 mM NaCl (Supplementary Fig ...
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bioRxiv - Bioengineering 2021Quote: The His-tag affinity-purified GINKO1 protein was further applied on the size exclusion chromatography Superdex200 (GE Healthcare) column pre-equilibrated with 25 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was further purified with gel filtration on a Hi-load 16/60 Superdex-75 column (GE Healthcare) in 25 mM Trizma (pH 7.5) ...
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bioRxiv - Neuroscience 2022Quote: ... NM- His was purified from the supernatant via IMAC using the ÄKTA pure protein purification system (GE Healthcare) together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare) ...
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bioRxiv - Immunology 2020Quote: ... Proteins were purified on the Ni resin using a commercial buffer set (His Buffer Kit, GE Healthcare, USA). In brief ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was loaded onto a His-Trap column using an ÄKTA protein purification system (GE Healthcare Life Sciences). The chromatography was run with a two-step gradient started with 100% starting buffer (500 mM sodium chloride ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... The fractions that contained the protein were pooled and subjected to Hi-trap ion exchange chromatography (GE healthcare) for further purification.
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to anion exchange chromatography (MonoS ...
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bioRxiv - Neuroscience 2023Quote: ... SNAP25 and Complexin proteins were further purified using gel filtration (Superdex75 Hi-load column, GE Healthcare, Chicago, IL), and Synaptotagmin-1 protein was subjected to the anion exchange column (MonoS ...