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Citations for GE Life Sciences :
201 - 250 of 4327 citations for Recombinant Human S100 Calcium Binding Protein A1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Cell Biology 2021Quote: SPR binding experiments were collected on a Biacore T200 Instrument (GE Healthcare). Anti-His6 monoclonal antibody (Abcam ...
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bioRxiv - Molecular Biology 2020Quote: ... Heats of binding were measured using a MicroCal iTC200 calorimeter (GE Healthcare) with a stirring rate of 1000 rpm at 27 °C ...
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bioRxiv - Molecular Biology 2019Quote: All binding measurements were conducted using a BIAcore T100 instrument (GE Healthcare). Biotinylated RNF43 ...
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bioRxiv - Genetics 2021Quote: We performed SPR binding analysis using a Biacore T200 instrument (GE Healthcare) at room temperature in HBS-EP(+ ...
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bioRxiv - Immunology 2021Quote: SPR binding studies were performed using a Biacore T200 (GE life sciences). All studies were performed at 25°C in running buffer consisting of 10mM HEPES ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody binding was detected by enhanced chemiluminescence (GE Healthcare; Buckinghamshire, United Kingdom). Protein quantification was performed using a GS-900 Calibrated Densitometer (Bio-Rad Laboratories ...
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bioRxiv - Pathology 2024Quote: ... Lysates were purified through binding to Fast flow Nickel sepharose (GE Healthcare) and washed with buffer (50 mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... binding curves were analyzed using BIAevaluation software (GE Healthcare, Marlborough, MA, USA) following standard procedures ...
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bioRxiv - Immunology 2019Quote: Binding to FcγRs was carried out using a Biacore T200 biosensor (GE Healthcare). Recombinantly expressed FcγRS (R&D systems or Sino Biologicals ...
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bioRxiv - Biochemistry 2020Quote: Measurements of heparin binding were performed in a Biacore T200 instrument (GE Healthcare) using a heparin-coated chip (Xantec) ...
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bioRxiv - Biochemistry 2020Quote: ... which allows affinity purification by binding to a StrepTactin Sepharose resin (GE Healthcare). Cells were grown in LB medium at 37°C with agitation to OD600nm = 0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2021Quote: ITC binding assays were carried out on a MicroCal ITC200 calorimeter (GE Healthcare) at 25 °C in a buffer containing 20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: All analytical SEC binding assays were conducted on an ÄKTAmicro system (GE Healthcare) using a Superose 6 Increase 3.2/300 column (Cytiva ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cancer Biology 2019Quote: ... Primary antibodies were detected by binding goat anti-rabbit IgG-HRP (NA934, GE Healthcare), goat anti-mouse IgG-HRP (NA931 ...
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bioRxiv - Biochemistry 2020Quote: Binding studies were performed at 25°C using a MicroCal iTC200 instrument (GE Healthcare). ANT(9 ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibody binding was followed by anti-mouse (GE Healthcare, catalog # NXA931V, lot # 17041890) or anti-rabbit IgG (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: SPR binding experiments were collected on a Biacore T200 or S200 Instrument (GE Healthcare). Neutravidin (Pierce ...
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bioRxiv - Cell Biology 2020Quote: ... Umbilical cords were flushed with Hank’s balanced salt solution with magnesium and calcium (HyClone™, GE Healthcare, Solingen, Germany) and PBS with magnesium chloride and calcium chloride (Merck KGaA ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2020Quote: ... Chemiluminescence signal from antibody binding was detected using ECL Select detection kit (GE healthcare, GERPN2235) with a ChemiDoc system (BioRad).
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bioRxiv - Biochemistry 2020Quote: ... The binding study was carried out at 25°C in PBS-P buffer (GE Healthcare). The heparin molecule or fondaparinux at different concentrations were flowed over the immobilized recombinant proteins at a flow rate of 30 μl/min with a contact time of 60 s and a dissociation time of 100 s ...
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bioRxiv - Biochemistry 2020Quote: Binding affinity of a repebody was determined through surface plasmon resonance (Biacore T200, GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2021Quote: RBD binding to ACE2 was analysed on a Biacore T200 instrument (GE Healthcare Life Sciences) at 37°C and a flow rate of 30 μl/min ...
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Species-specific protein-protein interactions govern the humanization of the 20S proteasome in yeastbioRxiv - Genetics 2022Quote: ... Primary antibody binding was followed by anti-mouse-IgG (GE Healthcare, catalog #NXA931V, lot #17193521) or anti-rabbit-IgG (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Equilibrium binding experiments were performed at 25°C using a Biacore T200 instrument (GE Healthcare) using PBS + 0.005% (v/v ...
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bioRxiv - Microbiology 2023Quote: Antibody binding properties were analysed at 25 °C using a Biacore 8K instrument (GE Healthcare) with 10 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: SPR studies for studying binding kinetics were carried out using BIACORE T200 (GE Healthcare, USA) with Ni-NTA research grade sensor chip (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: ... hippocampi were subjected to enzymatic digestion in Hanks′ Balanced Salt Solution (HBSS) medium without calcium and magnesium (Hyclone, GE Healthcare) supplemented with Trypsin (1x ...
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bioRxiv - Pathology 2023Quote: ... the EVs (resuspended in 100 µl phosphate buffered saline [PBS], without calcium and magnesium; GE Healthcare BioSciences, Pittsburgh, PA, USA) were incubated with 10 µM red fluorescent Lipophilic Tracer DiD (1,1’-dioctadecyl-3 ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... before binding to and NaCl gradient elution from HiTrap SP cation exchange chromatography columns (GE Healthcare). Fractions containing histones were pooled and dialyzed three times against water containing 5 mM β-mercaptoethanol and lyophilised for long-term storage at −80°C.
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bioRxiv - Microbiology 2020Quote: Binding affinities were measured by isothermal titration calorimetry (ITC) using a MicroCal iTC200 system (GE Healthcare) at 25°C using wild type truncated SPPV14 as well as two mutants SPPV14 Y46A and R84A in 25 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Analyte to ligand binding experiments were done in the Biacore 3000 instrument (GE Healthcare Life Sciences) at 25°C ...
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bioRxiv - Microbiology 2022Quote: ... The binding affinity was determined by and curves were generated by BIAEvaluation software (GE Healthcare, USA).
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bioRxiv - Cell Biology 2022Quote: ... RNA was purified using nucleic acid binding plates (96-well, 800 μl UNIFILTER Microplate, GE Healthcare) and was stored with RNAse-inhibitor at −80°C.
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bioRxiv - Immunology 2020Quote: ... The sensograms were fit in a 1:1 binding model with BIA Evaluation software (GE Healthcare). For epitope mapping ...
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bioRxiv - Neuroscience 2022Quote: ... The binding of HRP-conjugated secondary antibodies was revealed by ECL Plus (GE Healthcare Life Sciences) and chemiluminescence was measured using a ChemiDoc XRS apparatus (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... Antibody binding was visualized with Amersham ECL Western blotting detection reagent (GE Healthcare, Little Chalfont, UK).
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cell Biology 2022Quote: Binding experiments and kinetics assays were carried out using a BIACORE 3000 instrument (GE Healthcare, USA). Two different sensor chips were used ...
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bioRxiv - Biochemistry 2023Quote: SEC binding assays were performed using a Superdex 200 increase Hiload 10/300 column (GE healthcare) at a flow rate 0.4 ml/min with absorbances monitored at 280 nm ...
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bioRxiv - Cell Biology 2023Quote: ... The antibody binding was visualized using Amersham ECL Prime Western Blotting Detection Reagent (GE healthcare; #RPN2236) and imaged with the ImageQuant LAS400 Imaging unit (GE Healthcare).
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bioRxiv - Biochemistry 2023Quote: All the binding experiments were performed using the Superdex 75increase 10/300 GL column (GE Healthcare). 500 µl of reaction volume containing 20 µM Phospho-Ub (CBD tag)/phospho-UbN60D (CBD tag)/phospho-NEDD8 (His-SUMO tag ...