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Citations for GE Life Sciences :
601 - 650 of 4074 citations for Recombinant Human Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... 100nM Human DDX5 siRNA pool (GE Healthcare Dharmacon ...
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bioRxiv - Molecular Biology 2019Quote: ... and α-mouse HRP-linked F(ab’)2 (GE Life Sciences NA9310; 1:5000). Blots were visualized by chemiluminescence with the SuperSignal West Femto kit (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... For co-immunoprecipitation of endogenous SQSTM1/p62 the cell lysates were incubated for 2-4 h with specific antibody followed by 1-2 h with protein-G coupled Sepharose beads (GE Healthcare, 17-0885-01). To resolve protein complexes for denaturing immunoprecipitation ...
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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bioRxiv - Microbiology 2020Quote: ... Fixed cells were incubated with 2% ECL Prime Blocking Reagent (GE Healthcare, Piscataway, NJ, USA) in PBS at 25°C for 30 min ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were transfected with 40 nM small interfering (si)RNA (GE Life Sciences) and 2 μL (24-well plates ...
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bioRxiv - Cell Biology 2021Quote: cDNA encoding human Nup35 was amplified from MGC Human Nup35 sequence-verified cDNA (Accession number BC047029, GE Healthcare, Dharmacon), using primers that add an amino terminal HA tag ...
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bioRxiv - Molecular Biology 2021Quote: ... The full-length human eRF3a was expressed in insect Sf21 cells and purified via affinity chromatography using a HisTrap HP column (GE Healthcare) followed by anion-exchange chromatography on a MonoQ column (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... These cells were isolated from human blood by density-based centrifugation following layering of the blood onto Ficoll-Hypaque plus (GE healthcare). Cells were isolated and washed three times in RPMI (Gibco ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Biophysics 2021Quote: ... the FLAG-tagged Ubr1 was eluted with 1mg/mL FLAG peptide and further purified by a Superose 6 size-exclusion column (GE Healthcare) equilibrated in a buffer captaining 50 mM HEPES ...
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bioRxiv - Molecular Biology 2019Quote: ... and trimeric complexes were affinity purified via 6xHis-tagged RING1B on Ni2+-charged IMAC Sepharose 6 Fast Flow resin (GE Healthcare). 10 mM imidazole was added to lysates during binding ...
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bioRxiv - Developmental Biology 2019Quote: Full-length dP75 protein with GST tag was purified from BL21 E.coli by using Glutathione Sepharose 4B GST-tagged protein purification resin (GE healthcare, 17075601) and following the protocol in GE healthcare webpage ...
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bioRxiv - Biochemistry 2020Quote: ... Both the His-tagged and GST-tagged variants of PrgA were subsequently loaded on a Superdex- 200 16/600 size exclusion chromatography column (GE Healthcare) in 10 mM HEPES/NaOH (pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: A mixture of 0.5 μM GST or GST tagged cargo receptors and different ATG proteins was incubated with 10 μL Glutathione Sepharose beads (GE Healthcare) in a reaction buffer containing 20 mM HEPES at pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... His- or GST-tagged proteins were captured from the lysate by the addition of 100 μL Ni Sepharose High Performance beads (GE Healthcare) or Glutathione Sepharose High Performance beads (GE Healthcare) ...
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bioRxiv - Systems Biology 2020Quote: ... The supernatant was transferred to a fresh tube and added with 500 μl of 50 % (vol/vol) GST tagged glutathione-sepharose slurry (GE Healthcare) in lysis buffer and rotated at 4 °C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... The N-terminal thioredxin-His6-tagged proteins were purified using Ni-NTA agarose affinity column followed by size-exclusion chromatography (Superdex 200 column, GE Healthcare) in the buffer containing 50 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 6xHis tagged GRP78 was purified by HisTrapTM HP column (GE Hearlthcare, Uppsala, Sweden) and HiTrapTM Q HP (GE Healthcare, Uppsala, Sweden). The fractions containing GRP78 protein were combined and concentrated by centrifuging through 30-kDa Amicon ultra-15 centrifugal filter units (EMD Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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Bacterial killing by complement requires direct anchoring of Membrane Attack Complex precursor C5b-7bioRxiv - Immunology 2019Quote: ... for 2h at 4 °C in Tris/NaCl buffer (50 mM Tris/300 mM NaCl and the tagged protein was purified over a HisTrap FF column (GE Healthcare). GGG-azide labelled proteins were washed with Tris/NaCl buffer and concentrated to 25 µM on a 30 kDa Amicon Tube (Merck Millipore ...
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bioRxiv - Microbiology 2021Quote: ... His6-tagged SECTO-5GS-1TD0 spike protein was extracted from the supernatants using an immobilized Ni SepharoseTM Excel column (GE Healthcare) and eluted with 500 mM Imidazole prior to SEC ...
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bioRxiv - Microbiology 2022Quote: ... The 6xHis-tagged protein was purified from the insoluble fraction of a separately induced culture using Ni-NTA beads (GE Healthcare) with 250 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein was concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 25 mM Hepes pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... The SUMO-tagged or cleaved protein were concentrated and loaded onto a Superdex 200 16/60 size exclusion (SEC) column (GE Healthcare) pre-equilibrated at 4°C in 20 mM Hepes pH 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... the untagged protein was separated from cleaved tags and the 6xHis-tagged 3C protease by affinity chromatography using 5 ml HisTrap HP NTA column (GE Healthcare). If proteins were also tagged with MBP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... with 10 mM PTI and PTI alone in water (2 mL) were loaded into 2 mL mini-dialysis tubes with 1 kDa molecular weight cut-off (GE Healthcare Bio-sciences Corp. USA). The tube was immersed in a 200 mL closed Duran flask which was placed in a water bath at 37°C for the duration of the experiment ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Quality control of A33-His was performed by SDS-PAGE and size exclusion chromatography (Superdex75 10/300GL, GE Healthcare). The protein was digested with PNGase F (New England BioLabs ...
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bioRxiv - Biochemistry 2020Quote: ... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
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bioRxiv - Biochemistry 2021Quote: The filtered lysates were subjected to IMAC using a His-Trap 5 mL Nickel column (GE Healthcare Life Sciences) on an ÄKTA Pure system (GE Healthcare Life Sciences) ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was incubated at 10°C for 3h and then loaded onto a His Spintrap column (GE Healthcare). After 1-hour incubation ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were filtered (0.45 μm) to remove residual cellular debris and loaded onto a His-Trap Column (GE Healthcare) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The concentrated sample was further purified over a Superdex 200 Hi-load 16/60 gel filtration column (GE Healthcare) pre-equilibrated with 20 mM potassium phosphate (pH 7.4) ...
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bioRxiv - Biophysics 2019Quote: ... The eluted proteins from Ni-NTA column were loaded onto a Hi-load 16/60 Superdex 200 (GE Healthcare) gel filtration column pre-equilibrated with 25mM Tris pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: The interaction between PttP1 and PttP2 was studied by a pulldown assay using His SpinTrap™ columns (GE Healthcare) with a bed volume of 200 μl ...
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bioRxiv - Microbiology 2021Quote: ... The proteins were further purified by Ni-NTA immobilized metal-affinity chromatography using His-Trap HP columns (GE Healthcare) followed by size exclusion chromatography ...
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bioRxiv - Biophysics 2021Quote: ... tag cleavage and dialysis followed by gel filtration on a Superdex 75 Hi Load column 16/60 (GE Healthcare) pre-equilibrated with 20mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... and loaded to a Hi-Load 16/600 Superdex-200 pg (preparation grade) size-exclusion chromatography column (GE Healthcare) equilibrated with the gel filtration buffer (100 mM NaCl ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed size-exclusion chromatography of His-tag purified coGFP variants on an Äkta Pure chromatography system (GE Healthcare), with a Superdex 75 10/300 Increase column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Protein TLR827–827-PA-His was purified with IgG beads (Cat#17-0969-01, GE Healthcare, Connecticut, CT, USA) and resuspended in 200 μl of NT2 buffer 66 and used for miRNA-protein co-IP ...
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bioRxiv - Biochemistry 2023Quote: ... most of the proteins were loaded onto a Hi-trap Q HP anion exchange column (GE Healthcare, cat. 17115401). The final purification step involved using a size exclusion column ...
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bioRxiv - Microbiology 2024Quote: ... His-2xFYVE-containing fractions were pooled and subjected to size exclusion chromatography using a Superdex 75 column (GE HealthCare). Finally ...
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bioRxiv - Biochemistry 2024Quote: ... All purification steps were performed at 4°C and the pre-packed amylose column (5ml Hi-Trap GE healthcare) was equilibrated with 25 mL Amylose column A Buffer ...
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bioRxiv - Biochemistry 2024Quote: Native and selenomethionine-substituted BT4246 proteins were purified using a 5 ml Hi-Trap metal affinity cartridge (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant protein was purified by chromatography using a Nickel-loaded Hitrap Chelating HP column (GE Healthcare). Unbound material was extensively washed using Tris 20mM pH8 ...
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bioRxiv - Immunology 2021Quote: Purification of the recombinant antibodies was conducted on a ÄKTAprime plus system (GE Healthcare Life Sciences) at 4°C using a 1 mL HiTrap® Protein A HP pre-packed Protein A Sepharose® column (Cytiva) ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant APE1 were then further purified by Superdex 200 increase 10/300 chromatography (GE Healthcare) in the buffer containing 20 mM Tris-HCl (pH 7.0) ...
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bioRxiv - Microbiology 2019Quote: ... Recombinant WT and mutant INs were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... The recombinant proteins were purified by Ni2+-affinity chromatography using Histrap HP FPLC columns (GE Healthcare) and a size-exclusion chromatography using a HiLoad 16/60 200 PG column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... Recombinant IgG mAbs were purified using HiTrap Protein A columns (Cytiva/GE Healthcare Life Sciences, 17040303).