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Citations for GE Life Sciences :
651 - 700 of 3667 citations for Recombinant Human Programmed Cell Death 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... The lysate was clarified by centrifugation at 47,000 x g for 45min and applied to 5 ml His-Trap columns (GE Healthcare). After washing with a buffer excluding urea ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was filtered in 0.45 µm filter and loaded into a 5 ml His-trap FF column (GE Healthcare) pre-equilibrated with buffer A (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: ... Overexpressed recombinant proteins in supernatants were purified by AKTA start affinity chromatography system HiTrap Mabselect (GE Healthcare) according to the manufacturer’s instructions (S1 Fig).
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bioRxiv - Biochemistry 2019Quote: ... The recombinant Mtb-MenD protein was purified by IMAC with 5 mL HisTrap HP columns (GE Healthcare) using an imidazole gradient of 20–500 mM over 90 mL ...
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bioRxiv - Microbiology 2021Quote: Recombinant CNF1 toxin was fluorescently labeled with Cy3 (Cy®3 Mono 5-pack, GE healthcare, PA23001) according to the vendor’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... recombinant protein was purified from the filtered culture supernatant using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant WT and mutant IN molecules were analyzed on Superdex 200 10/300 GL column (GE Healthcare) with running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Molecular Biology 2020Quote: ... The GST part was cleaved off from the recombinant protein using PreScission Protease (GE Healthcare Life Sciences) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
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bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
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bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
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bioRxiv - Molecular Biology 2020Quote: ... 250 μl of antiserum were coupled to 50 μl of recombinant protein A-sepharose (rPAS, GE Healthcare). Antibody beads were washed three times in PBS and treated with 5 μl fresh rPAS ...
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Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... Recombinant D9 was purified sequentially by a Ni-NTA column followed by a heparin column (GE Healthcare) and a GE Superdex 75 16/60 gel filtration column to separate aggregates and exchange into crystallization buffer (10 mM MES pH 5.6 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
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Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
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bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
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bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Immunology 2022Quote: Cells were isolated from human blood using density gradient centrifugation agent Ficoll®-Paque Premium (17-5442-02, GE Healthcare, SIGMA, Darmstadt, Germany). Monocytes were plated at 3×105 cells per well in plastic 24 well plates ...
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bioRxiv - Immunology 2023Quote: ... freshly isolated mononuclear cells were separated from human blood by density centrifugation over Ficoll-Paque Plus (GE Healthcare, Chalfont St. Giles, United Kingdom). CD4+ T cells were subsequently purified by negative selection in an Automacs (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2020Quote: ... cells were reverse-transfected using DharmaFECT 1 (GE Healthcare) and then transfected again 24 h later according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... pGEX-4T-1 cells were obtained from GE Healthcare. All other reagents and chemicals used were of the highest grade available.
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bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Biophysics 2019Quote: ... for 20 min at 4 0C and histidine tagged protein was recovered from the lysate using a HiTrap TALON Crude Co2+ column (GE Healthcare, Piscataway, NJ) as described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Biophysics 2023Quote: ... His6-tagged fusion proteins were isolated from the resulting supernatant by affinity chromatography using Ni2+ Sepharose High-Performance beads (GE Healthcare, Pittsburgh, PA). Bound fusion proteins were washed with 200-300 mL of column Buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... and 6xHis-SMT3/SUMO tagged IMPDH2 was initially purified by Ni affinity chromatography using either a HisTrap FF column (GE Healthcare Life Sciences) on an Äkta Start chromatography system or a handpacked HisPur™ Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Cell Biology 2020Quote: ... The clear supernatant layer was collected and run over five serially connected Hi-Trap desalting columns (GE healthcare, 17-1408-01) in buffer A supplemented with 2 mM DTT and 14% glycerol ...
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bioRxiv - Microbiology 2021Quote: ... Further purification was carried out by size exclusion chromatography using a Superdex™ 200 Hi Load 16/60 column (GE Healthcare). Proteins were eluted with SEC buffer (25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysed cells were centrifuged 20,000 xg for 30 minutes at 4C and soluble lysate was filtered prior to IMAC purification using 2×5mL His-Trap columns (GE Healthcare) affixed to an Akta Pure ...
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bioRxiv - Biophysics 2019Quote: ... Sample was further purified from free labelled peptide and Sortase by size-exclusion chromatography using a Hi/Load 16/600 Superdex 75 column (GE Healthcare) previously equilibrated with ZapA storage buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... except that purification by size exclusion was omitted and the solubilized inclusion bodies were pre-cleaned by passage over a Hi-Trap Q-Sepharose column (GE healthcare) before loading onto a Hi-Trap SP-Sepharose column (GE healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were concentrated using Amicon Ultra-15 concentrators and further separated by HiTrap Heparin HP column and Hi-load 16/600 superdex 200 column (GE Healthcare). Fractions from the column were pooled and subjected for SEC-MALS analysis to determine their oligomeric status ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted proteins were then treated overnight with recombinant HRV 3C protease to remove the N-terminal His-tag and further purified via size exclusion chromatography on a HiLoad 26/600 Superdex 75 column (GE Healthcare) operating with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2021Quote: ... The liposomes were then purified by gel filtration using a Hi Prep 16/60 Sephacryl S-300 HR column (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Pyo S8 DNase domain was separated from its cognate immunity protein by loading the sample onto a 5 ml Hi Trap Chelating HP column (GE Healthcare) equilibrated with denaturing buffer ...
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bioRxiv - Plant Biology 2020Quote: ... The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare) again ...
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bioRxiv - Plant Biology 2020Quote: ... Anti-NPR1 antibodies were purified from antisera by affinity chromatography using a tNPR1-His-immobilized column (Hitrap NHS-activated HP; GE Healthcare). To prepare for the tNPR1-His-immobilized column ...
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bioRxiv - Microbiology 2020Quote: ... The elution fractions were concentrated and (His)6-Rv3341 was further purified by gel exclusion chromatography using Superdex 200 Increase 10/300 GL column (GE Healthcare). The purified fractions were pooled ...
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bioRxiv - Biophysics 2020Quote: ... Immunoblots with anti-HA, anti-His or anti-SecB antibodies were visualized by Lumino image analyzer LAS-4000 mini (Cytiva, Tokyo, Japan (GE Healthcare)) using ECL Prime Western Blotting Detection Reagents (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... Size exclusion chromatography was performed to separate FLAG-Ddi1 from the cleaved SUMO protein or the full-length His-SUMO-FLAG-Ddi1 using a HiLoad 26/600 Superdex 200 pg column (GE Healthcare) in a buffer of 20 mM Tris HCl pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... The precipitants were dialyzed against a 400 times volume of PBS (-) plus Triton X-100 and then applied to a Nickel (Ni)-Sepharose Histidine-affinity column (His GraviTrap; GE Healthcare). Bound proteins were eluted with PBS (- ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Insoluble material was pelleted at 50,000 xg for 30 min at 4°C and the supernatant loaded directly onto a 5 ml His Trap HP (GE Healthcare) at 2 ml.min-1 and washed with 50 ml Buffer A at 4 m.min-1 at 4°C ...