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Citations for GE Life Sciences :
351 - 400 of 4154 citations for Recombinant Human Pro NGF Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250 µl slurry ...
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bioRxiv - Plant Biology 2020Quote: ... 50 □L of Protein G Sepharose (GE Healthcare) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Protein G–Sepharose (GE Healthcare, 17-0618-01). Dynabeads Protein G (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... using a protein purification system (ÄKTApurifier, GE Healthcare). Elution of the proteins was achieved by using a linear gradient of imidazole from 4 mM to 500 mM with sodium phosphate buffer B (20 mM Na2HPO4 x 2H2O ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10 ...
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bioRxiv - Molecular Biology 2020Quote: ... and purified on protein A column (GE healthcare). Proteins were buffer exchanged and concentrated in PBS pH7.4.
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bioRxiv - Biophysics 2020Quote: ... The protein A sensor chips (GE Healthcare Lifesciences) were loaded by injecting each ligand for 100 s until to reach a signal level between 100 and 120 arbitrary response units (R.U. ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 µl of Protein A agarose (GE Healthcare) was coupled with 2 µg of purified antibodies or 5 µl of antiserum ...
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bioRxiv - Biochemistry 2022Quote: Proteins were transferred onto nitrocellulose membrane (GE Healthcare) by wet blotting overnight at 8°C and 27 V ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were revealed with ECL Prime (GE Healthcare) and images were acquired using a LAS4000 Camera (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... Protein G SepharoseTM 4 Fast Flow (GE Healthcare), pre-blocked with 1 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred to nitrocellulose membranes (GE Healthcare), then blocked with 5% FBS or 5% milk in TBST and probed for 2 h with primary antibodies ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were electroblotted onto PVDF membrane (GE Healthcare), blocked with 5% nonfat milk in TBS with 0.1% Tween-20 for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... 10 μl Protein G-Sepharose matrix (GE Healthcare) was added which was previously equilibrated in TBS buffer and this mixture was further incubated for another 2 h on a rotating wheel at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) and blocked for 1 hour with Odyssey Blocking Buffer (Li-COR bioscience ...
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bioRxiv - Biochemistry 2020Quote: Protein A-Sepharose CL-4B beads (GE Healthcare) were prepared by first washing them twice in IPP150 buffer containing 20 mM Tris-base ...
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bioRxiv - Neuroscience 2019Quote: ... Fast-flow Protein-A sepharose beads (GE Healthcare) (250µl slurry ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein was transferred to nitrocellulose membrane (GE Healthcare). Membrane was blocked in 5% milk in TBST (TBS + 0.1% Tween-20) ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized using ECL reagents (GE healthcare) in a Biorad ChemiDoc XRS+ ...
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bioRxiv - Microbiology 2021Quote: ... and Äkta Pure protein chromatography system (GE Healthcare). Antibody fractions were dialyzed overnight in PBS and filter-sterilized though 0.22 μm Spin-X filters ...
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bioRxiv - Microbiology 2020Quote: ... Took 40μl protein G Mag Sepharose (GE Healthcare) and incubated with antibody at 4°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were bound to GST resin (GE Healthcare) and eluted in buffer A supplemented with 10 mM glutathione (pH-calibrated) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to nitrocellulose membrane (GE Healthcare) by wet electroblotting ...
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bioRxiv - Immunology 2020Quote: ... PGT151-coupled Protein A Sepharose beads (GE Healthcare) were added to the supernatant and rotated overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... proteins were transferred onto PVDF membranes (GE Healthcare). The human BCL2 protein was detected using the same mouse anti-human BCL2 antibody we use for flow cytometry followed by an HRP-linked anti-mouse Ig antibody (eBioscience ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare, Amersham Protran Supported 0.45 μm ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were purified by Ni-NTA (GE Healthcare). MSLN specific VH domains were in the pComb3x vector and purified from Escherichia coli HB2151 bacterial culture at 30°C for 16 h with stimulation by 1 mM IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards (GE Healthcare Gel Filtration Calibration Kits) were used for comparison of elution volumes and estimation of theoretical molecular masses (Supplementary Figure 10 ...
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bioRxiv - Immunology 2022Quote: ... and Protein G Sepharose (GE Healthcare cat.# 45000118) were mixed at 1:1 ratio before adding into the supernatant and rotating overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Monomeric protein was purified using Superdex200 (GE Healthcare) size-exclusion chromatography column on an AKTA Pure (Cytvia).
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bioRxiv - Immunology 2023Quote: ... proteins were transferred onto nitrocellulose membranes (GE Healthcare) at 10V constant voltage using OwlTM Semidry Electroblotting Systems (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were purified using glutathione resin (GE Healthcare) and eluted with lysis buffer supplemented with glutathione ...
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bioRxiv - Cancer Biology 2024Quote: ... and Protein G Sepharose beads (GE Healthcare, 17061801) overnight at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto nitrocellulose membranes (GE Healthcare) in a Tris-glycine transfer buffer containing 20% methanol ...
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bioRxiv - Microbiology 2020Quote: ... Purification of the 4D11 mAb was carried out by affinity chromatography using a Protein A/Protein G GraviTrap 1-mL column (GE Healthcare), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... by injecting 50 µL of 8 µM protein in Protein Buffer onto a 1 mL HiTrap Heparin HP column (GE Healthcare) and eluting with a 10 CV linear gradient from 150 mM – 1 M NaCl in 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... the reconstituted MBP-CENP-C1-600-Spy-CENP-C601-943-8xHis protein was separated from unligated protein fragments on a Superose 6 10/300 size-exclusion column (GE Healthcare) as described above ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Immunoglobulins were purified against Protein A from 1 ml of rabbit serum using a Protein A HP SpinTrap (28-9031-32, GE Healthcare) following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: The eGFP-AP fusion proteins and the eGFP-control were purified using the N-terminal His6-Tag and fast protein liquid chromatography (ÄKTAprime, GE Healthcare) with a prepacked ion affinity chromatography column (Ni-Sepharose 6 Fast Flow ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: Affinities and kinetic parameters of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... Kinetic parameters and affinities of protein-protein interactions were measured by surface plasmon resonance (SPR) analysis using a Biacore T100 biosensor (GE Healthcare). 2000 response units (RU ...
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bioRxiv - Microbiology 2021Quote: ... Protein size was analyzed using a calibration curve of proteins included in the high molecular weight calibration kit (GE Healthcare, Chicago). To test frozen samples for aggregation ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting protein/phospholipid complexes were separated from the remaining vesicles and soluble protein by SEC using a Superose 6 column (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... was used to determine the protein concentration by OD280 reading and protein was concentrated to 5 mg/mL using a Vivaspin protein concentrator spin column with a MWCO of 5kDa (GE Healthcare). Aliquots of 500 μL were stored at -80°C until use ...
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bioRxiv - Microbiology 2021Quote: ... Precipitation of radiolabeled SARS-CoV-2 Spike glycoproteins from cell lysates or supernatant was performed with CV3-25 in combination with a polyclonal rabbit antiserum raised against SARS-CoV-2 RBD protein for 1 h at 4°C in the presence of 45 μL of 10% protein A-Sepharose beads (GE Healthcare).
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed N-terminal MBP-tagged BBS proteins were purified with Dextrin SepharoseTM High Performance with MBP-tagged protein purification resin (GE Healthcare). One hundred micrograms of MBP and the MBP-tagged BBS proteins were individually mixed with 100 µg of bacterially expressed ARL3Q70L or ARL3T30N (see above ...
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bioRxiv - Cell Biology 2021Quote: ... Cleaved protein was further purified with size exclusion chromatography to remove any protein aggregates applied to a Superdex 200 column (GE Healthcare) in 25 mM HEPES (pH 7.5) ...
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bioRxiv - Plant Biology 2021Quote: ... The GST tag was cleaved from purified protein by elution of the tag-free protein from the column by overnight incubation with PreScission Protease (GE Healthcare) or alternatively by elution with 15 mM glutathione-containing purification buffer ...
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bioRxiv - Biochemistry 2020Quote: ... For X-ray crystallography the protein was further purified using fast-protein liquid chromatography on an AKTA Purifier UPC 10 (GE Healthcare) over a HiPrep 16/60 Sephacryl S-100 high-resolution gel filtration column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was loaded onto either a 1-ml Mono Q (ParAF proteins and ParBF1-42-mCherry-EcoRIE111Q) or Mono S (other ParBF proteins) 5/50 GL ion exchange column (GE Healthcare) pre-equilibrated with Mono Q/S-buffer containing a NaCl concentration to match the conductivity of the sample ...