Labshake search
Citations for GE Life Sciences :
251 - 300 of 364 citations for Recombinant Human PTPRC Fc chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
-
bioRxiv - Immunology 2020Quote: ... and thereafter were eluted with 20 mM reduced glutathione (recombinant GST or GST-fusion peptides) or incubated with PreScission Protease (GE Healthcare) overnight at 4 °C to remove the GST tag ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and used for recombinant protein purification either by using AKTA FPLC or on His-Trap columns (GE Healthcare) as per the manufacturer guidelines or by manually purifying pre-equilibrated Ni-NTA-agarose (Qiagen) ...
-
bioRxiv - Plant Biology 2023Quote: The GST-Tip6ΔSP-mCherry and GST-mCherry recombinant proteins were purified using glutathione sepharose 4B beads (GE Healthcare, Cat. No. 17075601) following the product protocol ...
-
Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... or THY1 human siRNA (L-015337-00-0005, GE Healthcare Dharmacon, Inc.). CAFs were used for experiments 72h after transfection.
-
bioRxiv - Microbiology 2020Quote: Binding kinetics and affinities for ACE2.Fc were assessed using surface plasmon resonance technology on a Biacore T200 instrument (GE Healthcare, Marlborough, MA) using a Series S CM5 sensor chip in filtered and degassed HBS-EP running buffer (10 mM HEPES ...
-
bioRxiv - Immunology 2021Quote: ... sterilized by filtration with a 0.4 μm filter and recombinant proteins were purified with HisTrap TALON FF crude or HisTrap Excel columns (GE Healthcare Life Sciences).
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA) following purification using HiTrap Protein-A column (GE healthcare, UK). The purified protein was sterile-filtered and stored in PBS.
-
bioRxiv - Immunology 2020Quote: ... Expression of the recombinant proteins was performed using ExpiCHOTM Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrapTM (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant Mt RnhC was concentrated and further purified by size exclusion chromatography using a 16/600Superdex® 200 pg columns (GE Healthcare). Mt RnhC eluted in a single peak and the fractions were pooled ...
-
bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified loading the cell lysate supernatant to a 5 ml HisTrap HP column (GE Healthcare, Little Chalfont, UK), washed with Buffer A ...
-
bioRxiv - Plant Biology 2023Quote: ... was induced with 0.5 mM IPTG (isopropyl-β-D-thiogalactopyranoside) and recombinant GST-CPK3 was purified by Glutathione Sepharose 4 Fast Flow chromatography (GE Healthcare) as described by the manufacturer.
-
bioRxiv - Cancer Biology 2023Quote: ... Escherichia coli cells by induction with 0.5 mmol/L isopropyl-β-D-thiogalactopyranoside overnight at 16°C and purified recombinant proteins using an ÄKTA fast protein liquid chromatography system (GE Healthcare). This was followed by affinity and size exclusion chromatography.
-
bioRxiv - Molecular Biology 2021Quote: ... column for Notch1fe (human version) or a Superdex 10/300 increase (GE Healthcare) column for Jagged1fe,HA and Notch1NRR ...
-
bioRxiv - Microbiology 2020Quote: Neutrophils isolated from fresh human blood using a Ficoll-Paque PLUS (GE Healthcare) gradient were resuspended in medium at a concentration of 5 × 106 cells/mL ...
-
bioRxiv - Cell Biology 2019Quote: ... human adult blood was collected and immune cells purified by Ficoll (GE healthcare). Mice were injected intravenously with 1×107 freshly isolated PBMCs or NK-depleted PBMCs obtained from the negative fraction of a positive selection (CD56+ ...
-
bioRxiv - Genomics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll-Paque (GE Healthcare) density centrifugation from buffy coats (American Red Cross ...
-
bioRxiv - Immunology 2019Quote: ... recombinant proteins secreted by transfected Leishmania cells were purified by liquid chromatography over His-Trap™ excel columns (GE Healthcare, Buckinghamshire, United Kingdom). Protein-containing fractions were pooled and dialysed two times against PBS (4 h and overnight) ...
-
bioRxiv - Immunology 2020Quote: ... and the C-terminal recombinant proteins containing a N-terminal 6xHis-tag were purified from the resulting supernatant using Hitrap Ni-NTA column (GE Healthcare, Uppsala, Sweden) according to the manufacturer instructions ...
-
bioRxiv - Immunology 2023Quote: ... The culture medium containing the secreted LALA-B3B9 recombinant immunoglobulin G (rIgG) was purified using the protein A affinity column (GE Healthcare, Chicago, IL). The eluted fractions from purification were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis to determine the purity of the purified antibody ...
-
bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were further purified by gel filtration on an ÄKTA Pure fast protein liquid chromatography (FPLC) system (GE Healthcare Life Sciences). The principal peaks were collected ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
-
bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
-
bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
-
bioRxiv - Cancer Biology 2019Quote: Human PBMC were isolated by centrifugation of whole blood on Ficol-Paque (GE Healthcare) gradient for 30 minutes (500g ...
-
bioRxiv - Physiology 2021Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque Plus (GE healthcare) density gradient centrifugation from donated blood of healthy volunteers ...
-
bioRxiv - Cell Biology 2022Quote: ... ON-TARGETplus Human TP53/PTGS1/PTGS2 siRNA SMARTpool by Dharmacon (GE Healthcare, Manchester, UK) was used ...
-
bioRxiv - Immunology 2022Quote: Human ACE2 was immobilized onto CM5 sensor chips using a Biacore 8K (GE Healthcare). Serial dilutions of purified S trimer or RBD of Omicron lineages were injected ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
-
bioRxiv - Physiology 2019Quote: Human PBMCs were isolated by Ficoll-Paque (GE Healthcare Life Sciences, Pittsburgh, PA, USA) centrifugation from blood drawn from donors recruited from the Boston community as part of the Phenogenetic Project and ImmVar Consortium ...
-
bioRxiv - Biochemistry 2022Quote: The pooled human neutrophils from healthy donors were enriched by Percoll (GE Healthcare, Sweden) density gradient centrifugation as previously described(34) ...
-
bioRxiv - Biochemistry 2023Quote: ... Human LSD1 were cloned into pGEXKG vectors and purified by GSH-Sepharose (GE Healthcare). For demethylation reaction ...
-
bioRxiv - Immunology 2023Quote: Human PBMCs were obtained through Ficoll density gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). Multicolor staining for cell populations was performed on cryopreserved samples ...
-
bioRxiv - Molecular Biology 2021Quote: ... The secondary antibody only negative controls established gating for each sample and NeuN labelled nuclei were then sorted and collected in tubes pre-coated with foetal calf serum (FCS; #SH30084.02, Hyclone, GE Healthcare Life Sciences, South Logan, Utah, USA). Purity checks were performed and all samples were validated at >97% purity.
-
bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA clones of human Sec22b and Syntaxin2 and Syntaxin3 were obtained from Dharmacon (GE Healthcare) (Sec22b ...
-
bioRxiv - Cell Biology 2020Quote: ... a set of ON-TARGET human siRNAs against Arp3 (J-012077-08, Dharmacon, GE Healthcare), Myh9 (J-007668-07 ...
-
bioRxiv - Biochemistry 2020Quote: ... The cDNA of human gelsolin (UniProt ID: P06396) was purchased from Open Biosystems (GE Healthcare). The fragment encoding subdomains 4-6 (residues 434-782 ...
-
bioRxiv - Immunology 2019Quote: Healthy human monocytes were isolated from whole blood by Ficoll-Paque PLUS (17144003, GE Healthcare) density centrifugation followed by magnetic selection with CD14 Human MicroBeads (130-050-201 ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: Human peripheral blood neutrophils were obtained from consenting healthy volunteers using discontinuous Percoll (GE Healthcare) density gradient centrifugation ...
-
Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... We isolated human peripheral blood mononuclear cells (PBMCs) using the Ficoll-paque gradient (GE Healthcare) and removed the adhered monocytes after induced plastic adherence with cell culture flasks ...
-
bioRxiv - Biophysics 2023Quote: Human peripheral blood mononuclear cells (PBMCs) were isolated using Ficoll-Paque (GE Healthcare Life Science) from buffy coats donated by healthy ...
-
bioRxiv - Molecular Biology 2020Quote: ... The human LRFN4 (last 40 amino acids) was cloned from LRFN4 cDNA (MHS6278-202832013, GE Healthcare) and the human LRFN5 (last 40 amino acids ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human T-cells were isolated from PBMCs by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare). CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11 ...
-
bioRxiv - Biophysics 2019Quote: Human SH-SY5Y neuroblastoma cells (ATCC, Manassas, VA) were cultured in DMEM/F12 (GE Healthcare, Boston, MA) with 10% fetal bovine serum (Gibco Co. ...
-
bioRxiv - Cell Biology 2021Quote: A commercially available siRNA pool was used to knockdown human RNF213 (#M-023324-02, GE Healthcare Dharmacon) in figure 2 ...
-
bioRxiv - Cell Biology 2020Quote: ... using siRNA SMARTpool ON-TARGETplus Human UBTD1 (80019) or ON-TARGETplus Non-Targeting Control siRNAs) (GE Healthcare). Lipofectamine 2000 was used for plasmid transfection according to the manufacturer’s instructions (11668 ...
-
bioRxiv - Microbiology 2020Quote: IgG was purified from heat-inactivated human plasma using Protein G HP spin trap columns (GE Healthcare) and then incubated with CaptureSelect IgA Affinity Matrix (ThermoScientific ...
-
bioRxiv - Biochemistry 2022Quote: ... and human FANCD2 was further purified by gel filtration chromatography on a Superdex 200 column (GE Healthcare) equilibrated with Buffer B containing 200 mM NaCl ...