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Citations for GE Life Sciences :
301 - 350 of 806 citations for Recombinant Human PRKD3 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Developmental Biology 2023Quote: ... His-tagged Prtg protein was labeled directly with Cy5 monoreactive dye (GE Healthcare). Each bait protein was screened in duplicate against a total number of 1241 secreted proteins distributed across two microarray slides ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... All the His-tagged proteins were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100 mM-500 mM Imidazole gradients ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins and His-Atg8 were affinity purified using Glutathione Sepharose 4 Fast Flow (GE Healthcare) or Ni-NTA agarose (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... The fusion proteins (GST-OsbZIP86 and MBP-OsSAPK10) were purified using Glutathione Sepharose 4B (GE Healthcare, USA) and Amylose Magnetic Beads (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... and KDM2B1–734 were cloned into pGEX-4T-1 (GST tag vector) (GE Healthcare, Marlborough, MA, USA). The BL21 strain of E ...
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bioRxiv - Molecular Biology 2019Quote: ... 40 μg of GST-FG-repeat were incubated with 50 μL Glutathione Sepharose 4B beads (GE healthcare) at 4°C for 3 h ...
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bioRxiv - Cell Biology 2021Quote: ... GST pulldowns were done as described in (Bertran et al., 2011) using glutathione agarose beads (GE Healthcare).
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bioRxiv - Plant Biology 2019Quote: ... to remove the GST tag and further purified by gel filtration (Superose 6, 10/30; GE Healthcare). For cryo-EM investigation ...
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bioRxiv - Synthetic Biology 2021Quote: ... a glutathione-S-transferase (GST) (64) and thrombin sequence derived from pGEX-2T (GE Healthcare, Chicago, IL), and a maltose binding protein (MBP ...
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bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... or GST-Bud61-364 were incubated with HBSEP-equilibrated glutathione-coupled sepharose beads (GE Healthcare, Freiburg, Germany), for 30 min under rotation in the cold ...
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bioRxiv - Neuroscience 2022Quote: ... GST beads - (Glutathione Sepharose 4b beads, particle size 45 µm - 165 µm, GE Healthcare 17-0756-05) were incubated with the lysate overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Fractions containing GST-protein were pooled and loaded to a 6 mL Resource S column (GE Healthcare) equilibrated with buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... coli (BL21 strain) were performed according to the GST Gene Fusion System Handbook (GE healthcare Life Sciences). Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... The PCR products were fused in-frame behind GST on a pGex2T vector (GE Healthcare, Buckinghamshire, UK). 6xHis-tagged fragments were amplified from yeast genomic DNA using primers containing SfiI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare) in NETN buffer (20mM Tris PH8 ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... coding sequences of AtCFI25a and AtCFI59 were cloned into pGEX5X-2 (GST) (Merck/GE Healthcare, Darmstadt, Germany) or pET30c (HIS ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.1LmM PMSF) and included 80 pmol of GST-fused protein immobilized on GSH beads (GE Healthcare), 1–8 µg of heparin sulfate ...
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bioRxiv - Biochemistry 2019Quote: His6-tagged proteins were purified by binding proteins to Ni-NTA columns (GE Healthcare) in buffer D (50 mM Tris pH 8.0 ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged PPARγ LBD protein was immobilized on an NTA sensor chip (GE Healthcare), which includes a reference flow cell for background subtraction ...
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bioRxiv - Bioengineering 2022Quote: ... We purified the N-terminal His-tagged proteins on a HisTrap column (GE Healthcare) and then by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Cell Biology 2019Quote: ... All poly-histidine tagged proteins were purified using the Ni-NTA agarose (GE Healthcare), and eluted with imidazole ...
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bioRxiv - Cell Biology 2021Quote: For SPR: MBP-tagged proteins were immobilised on Sensor chip CM5 (GE Healthcare, #BR100012) and purified untagged RAC1 Q61L was titrated at different concentrations and the response signals were recorded and fitted into a 1:1 binding model ...
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bioRxiv - Microbiology 2020Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Biochemistry 2022Quote: ... the His-tagged DnaK protein was purified using a HisTrap HP column (GE Healthcare) with 2×PBS buffer followed by further purification using a HiTrap Q column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using Glutathione Sepharose 4B (17075601, GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (30210 ...
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bioRxiv - Immunology 2023Quote: ... Fc-tagged ACE2 protein was then purified with a Protein A column (GE Healthcare) followed by size exclusion chromatography.
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were isolated using Nickel beads (His Mag Sepharose Ni, GE Healthcare) in the QIAGEN BioSprint machine ...
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bioRxiv - Cell Biology 2020Quote: ... and HA-catalase were isolated from the purified GST fusion proteins by cleaving with PreScission protease (GE Healthcare) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-fused IQGAP1-N and IQGAP1-C were purified using Glutathione Sepharose 4B (17-0756-01; GE Healthcare). Next ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) and the supernatant (clear lysate) was then loaded onto GST GraviTrap columns (GE Healthcare Life Sciences) that had been pre-washed with PBS and buffer B (PBS pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-TEV-FICD was then depleted by a 1 h incubation with GSH-Sepharose 4B matrix (GE Healthcare). AMPylation was confirmed as being stoichiometric by intact-protein mass spectrometry (LC-ESI-MS ...
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bioRxiv - Cell Biology 2021Quote: ... while the supernatant of GST-fusion protein homogenates were loaded onto pre-equilibrated glutathione SepharoseTM beads (GE healthcare), and incubated on an orbital rotator for an hour in cold room.
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bioRxiv - Cell Biology 2021Quote: ... GST-Bro1V fusion protein and mutants thereof were purified using the Glutathione Sepharose 4B (GE Healthcare, United Kingdom), treated with AcTEV protease (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... coli similar to the GST-MS2 procedure [20] and then purified on a glutathione-Sepharose column (GE Healthcare). Following elution ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL Glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2020Quote: ... Scd1 (aa 10-872) and Rga4 (aa 389-1150) were cloned into pGEX6P1 (GST tag) vector (GE Healthcare), expressed in BL21 (DE3 or Rosetta ...
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bioRxiv - Cell Biology 2020Quote: ... western blot based GST pull-down assay was performed in which Glutathione Sepharose® 4B beads (GE Healthcare) was coupled with 10 µg of purified r14-3-3I protein in buffer A (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and incubated with GST beads for 1h at 4°C on rotator (GE Healthcare). GST beads were washed three times with phosphate buffered saline (PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion protein was purified by affinity chromatography on glutathione-Sepharose (GE Healthcare Life Sciences (now Cytiva)) in 300 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was applied onto a self-packaged GST-affinity column (2 mL glutathione Sepharose 4B; GE Healthcare) and contaminant proteins were removed with wash buffer (1×PBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Recombinant proteins were purified using a HIS-Trap (GE Healthcare) or Amylose resin (New England Biosciences ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged Ola1p was purified via IMAC using an ÄKTA Pure FPLC system (GE Healthcare) equipped with a Ni-NTA column (1 ml HisTrap HP column ...
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...