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Citations for GE Life Sciences :
101 - 150 of 4633 citations for Recombinant Human PDCD1 Protein Fc Avi His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... soluble 6His-tagged proteins were purified using a NiNTA resine (GE Healthcare) and imidazole was removed by dialysis into a buffer containing 50 mM TrisHCl ...
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bioRxiv - Plant Biology 2020Quote: ... GST-tagged proteins were purified using Glutathione Sepharose 4B (GE Healthcare, USA). Protein concentration was determined using Bio-Rad protein assay (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Histidine tagged proteins were immobilized on a Ni2+-sepharose resin (GE Healthcare) to remove the 1 mM DTT (Dithiothreitol ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200-1500 response units (RUs) ...
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bioRxiv - Bioengineering 2023Quote: ... Ly6a-Fc fusion protein in HBS-P+ buffer (GE Healthcare) was immobilized to a protein A sensor chip at a capture level of approximately 1200 -1500 response units (RUs) ...
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bioRxiv - Microbiology 2020Quote: ... The protein was captured using a His-Trap column (GE Healthcare). The column was washed with 10 CV’s of wash buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured on Nickel columns (His Trap HP, GE Healthcare) and eluted with 500 mM Imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... Both untagged and His-tagged Impβ were further purified using a Superdex 200 16/60 gel filtration column (GE Healthcare) in 50 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... We purified recombinant proteins and MBP protein by a Hisprep IMAC column (GE Healthcare). Cis-fragments of target gene promoters (34-bp free probes of OsRFL containing the NRE motif and 23-bp free probes of OsMOC1 containing the WNNN (CCANTG ...
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bioRxiv - Cell Biology 2022Quote: ... and biotinylated proteins were immunopurified through Streptavidin-Mag Sepharose™ (GE Healthcare). After washing of the beads ...
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bioRxiv - Cell Biology 2019Quote: ... biotinylated proteins were incubated with pre-equilibrated streptavidin sepharose beads (GE Healthcare) (50 μl bead slurry per 4 mg lysate ...
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the bacterially expressed 6×His tagged ARL3 and its variants were purified with Ni SepharoseTM 6 Fast Flow beads (GE Healthcare) and cleaved with thrombin (Solarbio ...
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bioRxiv - Bioengineering 2022Quote: ... His-tagged Ri-boswitch-VLPs were purified using a Ni beads gravity column loaded with high-performance Ni beads (GE Healthcare). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... Immobilized metal ion affinity chromatography of the His-tagged Pdr5 was performed with a Zn2+ loaded 1 ml HiTrap Chelating column (GE Healthcare) and elution was carried out with a step gradient using low and high histidine buffers (50 mM Tris-HCl pH 7.8 ...
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bioRxiv - Microbiology 2021Quote: Purified His-tagged Pentamer was immobilized to a single flow cell of a NiNTA sensor in a Biacore X100 (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Biochemistry 2020Quote: ... The liberated affinity-tag and the His-tagged Senp1 protease were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75 or 200 pg ...
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bioRxiv - Biophysics 2020Quote: ... Bacterial biomass was harvested by centrifugation at 5,400g and His-tagged nanobodies were purified using HisTrap™ column chromatography (GE Healthcare) followed by a buffer exchange step to PBS using a HiTrap™ desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease as described previously32 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tagged sample was purified by immobilized metal affinity chromatography (IMAC) using Ni Sepharose Excel resin (GE Healthcare Life Sciences). The IMAC eluted sample was dialyzed and treated with His-tagged Human Rhinovirus 3C protease ...
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bioRxiv - Immunology 2023Quote: Recombinant 50 kDa and 39 kDa CHIT1 proteins were expressed as C-terminally 8xHis-tagged constructs in HEK293-6E cells and purified from the cell culture supernatant by His-Trap (GE Healthcare) affinity as described in [42] ...
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bioRxiv - Biochemistry 2023Quote: ... The liberated affinity-tag and the His-tagged SENP1 and 3C proteases were removed by a second round of affinity purification with HisTrap FF columns (GE Healthcare). All proteins were purified with a final size exclusion chromatography (HiLoad 16/600 Superdex 75pg ...
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bioRxiv - Physiology 2019Quote: ... GST-tagged protein from supernatant were purified using glutathione-Sepharose 4B (GE Healthcare). Sepharose bound proteins were used as the protein columns ...
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bioRxiv - Biochemistry 2021Quote: ... All other hexa-histidine-tagged proteins were purified using a HisTrap (GE Healthcare) affinity chromatography step followed by thrombin cleavage ...
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Microbiology 2019Quote: ... Fc conjugated protein concentration was quantified using NanoVue Spectrophotometer (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
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bioRxiv - Systems Biology 2021Quote: ... Biotinylated proteins were probed using Streptavidin-HRP (GE Healthcare Life Science, Cat# RPN1231) at 1:2000 in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... Biotinylated bait proteins were captured on a streptavidin-coated sensor chip (GE Healthcare). Approximately 400 RU of the negative control bait (biotinylated rat Cd4d3+4 ...
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bioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were enriched using Streptavidin-conjugated Sepharose beads (GE Healthcare Life Sciences). Beads were washed once with RIPA buffer ...
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bioRxiv - Immunology 2022Quote: ... The biotinylated proteins were further purified by gel filtration (S200, GE Life Sciences) in PBS buffer pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Biotinylated proteins were immobilized on a CAP chip (Biotin CAPture kit, GE Healthcare). As a blocking control anti-LILRB2 antibody ...
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bioRxiv - Immunology 2020Quote: ... Fc mutations to prevent dimerization were introduced into the Fc sequence.61 Proteins were purified using MabSelect resin (GE Healthcare). Proteins were biotinylated using BirA enzyme (BPS Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...