Labshake search
Citations for GE Life Sciences :
101 - 150 of 4256 citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2022Quote: ... The His-tagged proteins in the supernatants were purified using HIStrap FF columns (GE Healthcare) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... His-tagged proteins were purified from transient transfection supernatants on HisTrap HP columns (GE Healthcare) using an ÄKTAxpress (GE Healthcare ...
-
bioRxiv - Microbiology 2024Quote: ... The NRP2-Fc was purified using a Protein-A affinity column (GE Healthcare). NRP2-Fc point mutations were introduced by PCR mutagenesis using Kapa HiFi DNA polymerase (Kapa Biosystems) ...
-
bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates were centrifuged at 12,000 x g for 30 min and the supernatant was subsequently used for protein purification through a HisTrap column (His- tagged full-length proteins, GE Healthcare) or Amylose resin (MBP-tagged fragments ...
-
bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
-
A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were first purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
-
bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... Recombinant tau proteins were immobilized on the CM5 sensor chip (Biacore, GE Healthcare) via amine coupling ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
-
bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
-
bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
-
bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... Recombinant proteins were purified with a fast flow Ni-NTA column (GE Healthcare) and eluted by 20 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Microbiology 2023Quote: ... The recombinant CoxbHtrA protein was purified using an ÄKTA avant system (GE Healthcare) equipped with a 5 mL StrepTrap HP column (GE Healthcare) ...
-
bioRxiv - Cell Biology 2023Quote: Recombinant RAD51 proteins were spotted on nitrocellulose membrane (GE Healthcare, Little Chalfont, UK). Membranes saturation ...
-
bioRxiv - Neuroscience 2024Quote: ... recombinant IgLON5 or LG1 protein was spotted onto a nitrocellulose membrane (GE Healthcare), membranes were blocked with 1% BSA in PBS ...
-
bioRxiv - Immunology 2023Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). To produce antibody Fabs ...
-
bioRxiv - Microbiology 2024Quote: ... and recombinant protein was purified using glutathione sepharose beads (GE Healthcare Life Sciences).
-
bioRxiv - Developmental Biology 2020Quote: ... and GST-tagged target protein was purified using Glutathione Sepharose 4B (GE Healthcare, Marlborough, MA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2019Quote: ... and C-terminal 6xHis-tagged effector proteins were immobilised onto a NTA sensor chip (GE Healthcare). HMA protein was flowed over the immobilised effector at 30 µl/min (360 sec contact time and 180 sec dissociation time ...
-
bioRxiv - Developmental Biology 2019Quote: ... His6-SUMO-tagged proteins were purified by affinity chromatography using a HisTrap HP column (GE Healthcare), followed by the cleavage of the His6-SUMO tag using Ulp1 protease ...
-
ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... the mixtures were purified with Dextrin SepharoseTM High Performance MBP-tagged protein purification resin (GE Healthcare). Ten micrograms of proteins from elutes was resolved on 12% SDS-PAGE gels and visualized with Coomassie blue staining ...
-
bioRxiv - Cell Biology 2021Quote: ... His⨯6-tagged proteins in the supernatant were purified by Ni-NTA agarose beads (GE Healthcare). After extensive wash by a buffer containing 20 mM imidazole ...
-
bioRxiv - Microbiology 2020Quote: ... His-tagged proteins were purified from supernatants on HisTrap HP columns using an ÄKTApure (GE Healthcare) and resolved by gel filtration on a Superdex 200 Increase 10/300 column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... The His-tagged fusion proteins were purified by Ni2+-Sepharose 6 fast flow (GE Healthcare, USA) affinity chromatography followed by size-exclusion chromatography.
-
bioRxiv - Microbiology 2019Quote: ... The soluble His-tagged protein was purified using a HisTrap column (GE Healthcare Life Sciences, USA). Purity of the eluted protein was checked by SDS-PAGE gel staining with Coomassie Blue ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-tagged expressed proteins were purified using GST beads (Glutathione Sepharose 4B; GE Healthcare Life Science). On the following day ...
-
bioRxiv - Microbiology 2022Quote: ... Filtered lysates with strep-tagged proteins were applied onto StrepTrap HP 1 ml columns (GE Healthcare). Subsequent protein separation was conducted using the ÄKTA start chromatography system (GE ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fc and non-digested IgG were removed via Protein A affinity chromatography (GE Healthcare). 2E10.E9 Fab was subsequently purified via size-exclusion chromatography (Superdex 200 Increase 10/300 GL ...
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Plant Biology 2020Quote: ... the cells were lysed by sonication and the fusion protein was purified using HisTrap HP histidine-tagged protein purification column of AKTA pure FPLC system (GE Healthcare, Pittsburgh, PA). The purified protein was further dialyzed overnight and further purified with HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... The proteins were harvested from the supernatant of cell cultures and purified by HisTrap Excel (for his-tagged proteins) or Protein G (for antibodies) columns (GE Healthcare Life Sciences). RHMS-1 (trimer) ...
-
bioRxiv - Microbiology 2020Quote: ... FLAG epitope-tagged protein levels were visualized using the Amersham ECL western blotting detection reagent (GE Healthcare). Thereafter ...
-
bioRxiv - Neuroscience 2022Quote: ... The Twin-Strep-tagged protein was purified via affinity chromatography using a StrepTrap HP column (GE Healthcare). Next ...
-
bioRxiv - Cell Biology 2020Quote: ... GST-tagged proteins were immobilized on 100 μl Glutathione–Sepharose beads in 1xPBS (GE Healthcare, Freiburg, Germany) directly from E ...
-
bioRxiv - Plant Biology 2020Quote: ... The recombinant proteins were purified with a pre-packaged HisTrap FF column (GE Healthcare). The His-MBP tags were cleaved by TEV protease overnight and removed by flowing through a HisTrap FF column (GE Healthcare ...
-
bioRxiv - Biochemistry 2022Quote: ... The recombinant proteins were purified using a Ni-NTA Superflow affinity column (GE Healthcare) and Source-Q (GE Healthcare ...
-
bioRxiv - Cancer Biology 2022Quote: ... Recombinant proteins were purified by affinity chromatography using glutathione (GSH) agarose beads (GE Healthcare) and dialyzed in mild lysis buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... The eluate containing recombinant proteins was passed through a PD-10 column (GE healthcare) to exchange buffer with Store Buffer (50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Plant Biology 2021Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 ml column (GE Healthcare), equilibrated with buffer A (50□mM NaH2PO4 (pH 8) ...
-
bioRxiv - Neuroscience 2021Quote: ... The recombinant protein was desalted with PD-10 column (SephadexTM G-25M, GE Healthcare), purified using a cation exchange column [HiTrap carboxymethyl (CM ...
-
bioRxiv - Molecular Biology 2019Quote: ... Recombinant proteins were purified by IMAC using an AKTA-pure-25 FPLC (GE Healthcare). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare) except for Fig ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant IgG was purified from the culture supernatants using protein A Sepharose (GE healthcare). The concentration of purified IgG was measured at OD280.
-
bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were immobilized on a HiTrap Talon crude 1 mL column (GE Healthcare), equilibrated with buffer A (50 mM NaH2PO4 (pH 8) ...