Labshake search
Citations for GE Life Sciences :
151 - 200 of 364 citations for Recombinant Human MME Fc Chimera since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... 250 µl of antiserum were coupled to 60 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed three times in CPB and supplemented with 6 µl fresh rPAS ...
-
bioRxiv - Pathology 2020Quote: ... Recombinant proteins were purified with a Ni-NTA resin and Q-Sepharose™ (GE Healthcare, Mississauga, ON). Endotoxin was removed using the Pierce™ High-Capacity Endotoxin Removal Resin (ThermoFisher Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins were then purified using immobilized metal affinity chromatography (His-Trap-Nickel-chelating columns, GE Healthcare) followed by treatment with TEV protease and a second immobilized metal affinity chromatography step to remove the His-tag ...
-
bioRxiv - Plant Biology 2021Quote: ... Recombinant protein MdSUMO2A-HIS and MdDREB2A were purified by HIS Sepharose beads (GE Healthcare, Fairfield, CT, USA), GST-MdCE was purfied by Pierce™ Glutathione Spin Columns (16105 ...
-
bioRxiv - Biophysics 2021Quote: ... Recombinant myosin was purified from clarified lysate by using the AKTA purify system as follows (GE Healthcare). First ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant proteins were purified by nickel affinity chromatography using HisTrap Column (GE Healthcare Life Sciences Pittsburgh, PA). Rat polyclonal antisera against L ...
-
bioRxiv - Molecular Biology 2020Quote: ... 250 μl of antiserum were coupled to 50 μl of recombinant protein A-sepharose (rPAS, GE Healthcare). Antibody beads were washed three times in PBS and treated with 5 μl fresh rPAS ...
-
Structure of the poxvirus decapping enzyme D9 reveals its mechanism of cap recognition and catalysisbioRxiv - Biophysics 2021Quote: ... Recombinant D9 was purified sequentially by a Ni-NTA column followed by a heparin column (GE Healthcare) and a GE Superdex 75 16/60 gel filtration column to separate aggregates and exchange into crystallization buffer (10 mM MES pH 5.6 ...
-
bioRxiv - Biochemistry 2022Quote: Recombinant CrRPE1 was injected on a Superose 6 Increase 10/300 GL column (GE Healthcare, Chicago, Illinois) and isocratically eluted in 50 mM Tris-HCl (pH 7.5 ...
-
bioRxiv - Plant Biology 2022Quote: ... The recombinant protein GST-LSH10 was purified using Glutathione Sepharose 4B beads (GE Healthcare, 17-0756-01) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Recombinant CaM was purified using phenyl-sepharose resin (Phenyl Sepharose® 6 Fast Flow, GE Healthcare Inc.) as described (Bender et al. ...
-
Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosisbioRxiv - Biochemistry 2023Quote: ... Recombinant proteins were then concentrated and loaded on a Superdex 200 Increase 10/300 GL (GE Healthcare) pre-equilibrated in size-exclusion chromatography buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatants containing soluble recombinant protein were pulled-down with Glutathione (GSH) Sepharose 4FastFlow (GE Healthcare, 17-5132) for 3h at 4°C with gently rocking ...
-
bioRxiv - Molecular Biology 2023Quote: ... 170 µl of antiserum was coupled to 40 µl of recombinant Protein A Sepharose (rPAS, GE Healthcare). Antibodies bound beads were washed extensively in CPB and supplemented with 4 µl fresh rPAS ...
-
bioRxiv - Microbiology 2024Quote: Recombinant WT and mutant IN proteins were analyzed using Superdex 200 10/300 GL column (GE Healthcare) with the running buffer containing 20 mM HEPES (pH 7.5) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
-
bioRxiv - Immunology 2021Quote: ... Human antibodies purified by Protein-G (GE Healthcare, USA) affinity chromatography were stored at -80°C until use.
-
bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
-
bioRxiv - Biochemistry 2022Quote: ... Fc-fusion constructs of each receptor were captured using either a Series S Protein A sensor chip (GE Healthcare) or a Series S CM5 sensor chip (GE Healthcare ...
-
bioRxiv - Immunology 2022Quote: ... Fc fragments were purified from cell culture supernatant using a 5 ml HiTrap Protein G HP column (GE Healthcare). The supernatants for each mutant were processed as two separate batches ...
-
bioRxiv - Plant Biology 2019Quote: ... Glutathione S transferase (GST)-tagged recombinant proteins were purified using glutathione sepharose 4B (GE Healthcare, #17-0756-01) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: Purified recombinant antibodies were assessed by SDS-PAGE under nonreducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
-
bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
-
LIR-dependent LMX1A/LMX1B autophagy crosstalk shapes human midbrain dopaminergic neuronal resiliencebioRxiv - Cell Biology 2019Quote: Recombinant ATG8 proteins were then coupled to cyanogen bromide-activated sepharose 4 Fast Flow (CNBr-sepharose, GE Healthcare) as described (Kavran & Leahy ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant proteins were further purified by anion-exchange chromatography on a Resource Q column (GE Healthcare, United States) that was pre-equilibrated with buffer A (20 mM Tris–HCl ...
-
bioRxiv - Neuroscience 2020Quote: ... re-suspended in ultrapure water and pre-cleared with recombinant protein A sepharose (GE Healthcare, Chicago, IL, USA). Immunoprecipitation of Aβ species from the media was performed with the monoclonal anti-Aβ clone W0-2 (MABN10 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Recombinant NS1-6xHis protein was further purified by gel filtration using an AKTA pure chromatograph (GE Healthcare, USA). An XK 16/60 Chromatographic Column ...
-
bioRxiv - Immunology 2019Quote: ... The VLP recombinant proteins were further purified by size exclusion chromotogrophy (Superose 6, 10/300 GL;GE Healthcare). The level of endotoxin in all the purified VLPs was measured with an ELISA-based endotoxin detection assay (Hyglos ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant proteins were purified directly from the filtered culture supernatants using HisTrap Excel columns (GE Healthcare Life Sciences). Each column was stored in 20% ethanol and washed with 5 column volumes (CV ...
-
bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
-
bioRxiv - Neuroscience 2022Quote: ... The recombinant protein in the supernatant of the cell lysate was mixed with Glutathione Sepharose resin (GE Healthcare) and the mixture was washed extensively in 10mM HEPES pH 7.3 ...
-
bioRxiv - Biochemistry 2023Quote: ... The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare). Recombinant His-tagged PHB1 was incubated with genistein beads (0.5 mg ...
-
bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
-
bioRxiv - Immunology 2023Quote: ... Recombinant HA molecules were purified from these culture supernatants by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with imidazole gradient ...
-
bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
-
bioRxiv - Biochemistry 2020Quote: ... ACE2-Fc proteins were then purified by size-exclusion chromatography (SEC) using Superdex 200 Increase column (GE healthcare, Chicago, IL) and stored at −80°C until used in experiments.
-
bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...
-
bioRxiv - Cell Biology 2020Quote: ... Human RAB34 cDNA was obtained from GE Healthcare (MHS6278-202807876) and cloned into Gateway pENTR plasmid pDONR221 (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: Human PBMCs were isolated by Ficoll-Paque PLUS (GE Healthcare) density gradient centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... human C5b was immobilised on a CM5 chip (GE Healthcare). Flow cells were activated using a standard immobilisation protocol ...
-
bioRxiv - Molecular Biology 2021Quote: ... Over 9500 Response Units of the recombinant HDP protein were immobilized on S-Series CM5 sensor chip (GE Healthcare) using 10mM sodium acetate pH4.0 solution (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: Expression of recombinant HDP1-DBD motif was done using the Glutathione S-transferase (GST) gene fusion system (GE Healthcare). The pGEX-4T-1 plasmid was used as backbone for cloning the codon optimized sequence comprising the last 87aa of HDP1 ...
-
bioRxiv - Plant Biology 2021Quote: ... The recombinant proteins were then purified using a 1 ml HisTrap HP column and ÄKTA™ system (GE Healthcare) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2021Quote: Molecular masses of recombinant GgNAD-MEs were evaluated by gel-filtration chromatography on an ÄKTA purifier system (GE Healthcare) using a Biosep-SEC-S3000 column (Phenomenex) ...
-
bioRxiv - Neuroscience 2020Quote: ... Recombinant GST-tagged protein in the supernatant was purified using glutathione-Sepharose 4B beads (GE Healthcare, 17-0756-01), washed in 10 mM PBS (pH 7.4 ...
-
bioRxiv - Microbiology 2019Quote: ... The recombinant catalytic domain of PknB was purified using Glutathione Sepharose 4B GST-tagged protein purification resin (GE Healthcare) as previously described5.
-
bioRxiv - Epidemiology 2019Quote: Recombinant Schistosoma mansoni glutathione-S-transferase (GST) protein was expressed from pGEX 4T-2 plasmid (GE Healthcare, Piscataway, NJ) and purified as previously described [26] ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Hemolysate supernatants and purified recombinant hemoglobins were similarly desalted by passing through a PD-10 desalting column (GE Healthcare) equilibrated with 25 ml of 0.01 M HEPES/0.5mM EDTA (pH 7.4) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The cells were lysed and recombinant KRAS G12D was purified using a HisTrap HP (GE Healthcare, 29-0510-21) column ...