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Citations for GE Life Sciences :
101 - 150 of 4549 citations for Recombinant Human KLRD1 protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... GST tagged proteins were purified with Glutathione Sepharose 4B beads (10250335, GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... tagged proteins were affinity-purified with a HisTrap HP nickel column (GE Healthcare) or glutathione Sepharose 4B resin (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... for the MBP-tagged proteins or glutathione sepharose resin (GE Healthcare Life Sciences) for the GST-tagged proteins ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged proteins were immobilized on glutathione sepharose beads (GE Healthcare, Freiburg, Germany) directly from E ...
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bioRxiv - Biochemistry 2022Quote: ... Tagged proteins were purified by affinity chromatography (HisTrap resp. StrepTrap, both GE Healthcare) with elution buffer containing 200 mM imidazole respectively 2.5 mM d-desthiobiotin ...
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bioRxiv - Cell Biology 2023Quote: GST-tagged proteins were affinity-purified using glutathione-Sepharose 4B beads (GE Healthcare) according to the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using glutathione agarose beads (GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (QIAGEN) ...
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bioRxiv - Biochemistry 2023Quote: ... All the GST-tagged proteins were bound to GSTrap HP columns (GE Healthcare) and were eluted with 10 mM reduced glutathione ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... 200 pmoles of purified proteins were incubated with immobilized GST-tagged protein to glutathione sepharose beads (GE Healthcare) at 4°C for binding reactions (for 2h) ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was passed through a Hi-Trap desalting column (GE Healthcare) and an S75 gel filtration column equilibrated in buffer containing 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... the protein was desalted using a Hi-Trap desalting column (GE Healthcare). After desalting ...
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bioRxiv - Molecular Biology 2020Quote: ... The soluble fractions containing His-tagged PhoH2 or PhoH2-R339A were purified by IMAC on a HisTrap HP column (GE Healthcare, UK). The protein fractions were purified further by size exclusion chromatography ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a HiTrap TALON column and eluted with 50 mM sodium phosphate ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Recombinant protein was first purified on HisTrap HP column (GE Healthcare) using Buffer A (50mM Tris Base ...
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bioRxiv - Synthetic Biology 2023Quote: ... recombinant proteins were purified using Ni-Sepharose affinity chromatography (GE Healthcare). Final elution was carried out at 250 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were purified by glutathione Sepharose 4B (GE Healthcare) or Amylose Resin High Flow (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: GST-tagged mammalian ATG8 fusion proteins were cloned into pGEX-4T-1 (GE Healthcare) and expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biophysics 2021Quote: ... The GST tagged Gbp2 proteins were purified using glutathione sepharose 4B resin (GE Healthcare) followed by size exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: GST-tagged proteins were purified using affinity chromatography with Glutathione Sepharose beads (GE Healthcare) in a cold room ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged PPARγ LBD protein was immobilized on an NTA sensor chip (GE Healthcare), which includes a reference flow cell for background subtraction ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tagged proteins were absorbed with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5hrs and recovered by 3C protease cleavage at 4°C overnight in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged proteins were purified with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01), washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... All poly-histidine tagged proteins were purified using the Ni-NTA agarose (GE Healthcare), and eluted with imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... GST-tagged proteins were captured by incubation with 20 μl glutathione sepharose (GE Healthcare) beads for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: For SPR: MBP-tagged proteins were immobilised on Sensor chip CM5 (GE Healthcare, #BR100012) and purified untagged RAC1 Q61L was titrated at different concentrations and the response signals were recorded and fitted into a 1:1 binding model ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using Glutathione Sepharose 4B (17075601, GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (30210 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants of GST-tagged protein homogenates were loaded onto glutathione SepharoseTM beads (GE healthcare). Prior to the elution ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare) via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... Recombinant antibodies were purified from supernatants using protein-G beads (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange on a HiPrepTM 26/10 desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant protein was then isolated using Ni-NTA resin (GE healthcare) and eluted with a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were purified on a HiTrap SP HP column (GE Healthcare) and subsequently on a HiTrap Q HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant proteins were first purified using a Ni-NTA column (GE HealthCare) and the His-GST tag was removed by cleavage with 3C-PreScision protease and subsequent incubation with Ni2+ beads to remove the uncleaved protein and the tag from the solution ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).