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Citations for GE Life Sciences :
401 - 450 of 1265 citations for Recombinant Human Interleukin 17 Receptor A Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and the supernatant is filtrated and applied to HisTrap™ FF (17-5319-01, GE healthcare, USA) for preliminary purification ...
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bioRxiv - Biochemistry 2020Quote: ... DBCO beads were synthesized in house: NHS-activated Sepharose™ fast flow (#17-0906-01, GE Healthcare) was incubated to varying concentrations of dibenzocyclooctyne-amine (DBCO-amine ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were run on a HiPrep S16/60 Sephacryl S-200 HR (GE Healthcare, #17-1166-01) to separate the proteins by molecular weights and then verified by SDS-PAGE (Figure S1) ...
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bioRxiv - Cancer Biology 2020Quote: ... Mononuclear cells (MNC) were extracted from fresh BM using Ficoll-Paque Plus (GE Healthcare, #17-1440-02). Bone marrow MNCs were also extracted from two patients (ALL10 and ALL12 ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a HisTrap column (5 mL, cat. No. 17-5248-01, GE Healthcare) pre-equilibrated with lysis buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Filtered supernatant was passed through pre-equilibrated Hitrap Heparin columns (#17-0406-01; Ge HealthCare Life science), followed by a wash with Wash Buffer 1 (20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... The immuno-complexes were collected with 50 μl of protein A sepharose (17-0780-01, GE Healthcare) at 4 °C for two hours with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... This was then coupled onto NHS-ester Sepharose 4 Fast Flow resin (GE Healthcare, 17-0906-01) for 4 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... The clarified lysates were incubated with glutathione sepharose (1 ml bed volume; GE Healthcare #17-0756-01), washed and eluted by the addition of 2 x 1.5 ml of 10 mM reduced L-glutathione (Sigma #T6066 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to glass slides previously treated with Repel Silane (GE Healthcare Life Sciences, 17-1332-01) and washed with ethanol ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were further purified by centrifugation over a Percoll gradient (GE Healthcare, 17-0891-01, Sigma Aldrich).
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bioRxiv - Neuroscience 2022Quote: ... Cells were separated from myelin and debris by 30% isotonic percoll gradient (#17-0891-01, GE Healthcare) in Myelin Gradient Buffer (MGB) ...
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bioRxiv - Immunology 2022Quote: ... The immune cells were obtained by density gradient centrifugation with Percoll (17-0891-09, GE Healthcare, USA) according to the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant was mixed with 50 µl of streptavidin sepharose (Cat. No. 17-5113-01, GE healthcare), shaking at 4°C for 3 h after being washed with PBS once ...
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bioRxiv - Plant Biology 2020Quote: The GST- and MBP-fusion proteins were separately purified using Glutathione sepharose (GE Healthcare, 17-5132-01) and Amylose resin (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... the protein was loaded onto a 1 ml HiTrap Heparin HP column (GE Healthcare, 17-0406-01) and eluted with a linear gradient from buffer A (20 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Size exclusion using a HiLoad 26/60 Superdex 75 prep grade column (17-1070-01, GE Healthcare) was necessary to further purify IF1 from contaminants of higher molecular weight ...
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bioRxiv - Immunology 2020Quote: ... The resuspended cells were subjected to lymphocyte enrichment using Ficoll-Paque Plus (17-1440-02, GE Healthcare). The decidual leukocytes were further purified by fluorescent activated cell sorting with 7-AAD (420404 ...
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bioRxiv - Biochemistry 2021Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2021Quote: ... Clarified dialysate was then bound to a MonoS cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Immunology 2021Quote: ... IFN-α2 protein was column-purified using Nap-5 columns (GE Healthcare, Chicago, IL; #17-0853-01), incubated with 2.5ul serum ...
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bioRxiv - Microbiology 2021Quote: ... and followed by Superose 6 10/300 gel filtration column chromatography (GE Healthcare, Cat# 17-5172-01). Proteins were then concentrated and the concentrations were determined using BCA protein concentration kit (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... GST beads - (Glutathione Sepharose 4b beads, particle size 45 µm - 165 µm, GE Healthcare 17-0756-05) were incubated with the lysate overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Eluted samples were further purified using the HiTrap™ Q HP column (GE Healthcare, 17-1154-01). The His-LC3B-tag was cleaved using human ATG4B protease at 25°C overnight45 ...
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bioRxiv - Cell Biology 2022Quote: ... and then combined with 60 μl of washed Protein A–Sepharose beads (17-0780-01, GE Healthcare) for 1 hour on a rotator at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was collected and mixed with fresh 80% Percoll (GE Healthcare, Cat. no. 17-5445-02) prepared in mitochondrial dilution buffer (1000 mM sucrose ...
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bioRxiv - Molecular Biology 2022Quote: ... Clarified supernatants were then incubated with 150 mL slurry of IgG beads (GE Healthcare, 17-0969-02) for 2h at 4 °C on a turning wheel ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was removed by resuspending pelleted cells into 30% isotonic Percoll (GE Healthcare, Cat#17-5445-02) and centrifuging at 300g for 15 minutes at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified dialysate was then bound to a MonoS cation exchange column (GE Healthcare, Cat# 17-5168-01) equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2023Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then purified using a ResourceTM Q 6ml column (17-1179-01, GE Healthcare, Uppsala, Sweden) and a Superdex 200 Increase 10/300 column (28-9909-44 ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysates were incubated with 70⍰μl of streptavidin beads (5⍰ml; 17-5113-01, GE Healthcare) at 4° for 3⍰h ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... the dialyzed protein was applied to a 5 ml HisTrapTM column (GE Life Sciences, 17-5248-02), equilibrated with dialysis buffer ...
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bioRxiv - Cell Biology 2023Quote: ... and loaded onto a 24 mL Superdex 200 10/300 GL (GE Healthcare, Cat# 17-5174-01) size exclusion column equilibrated in 20 mM Tris [pH 8.0] ...
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bioRxiv - Cell Biology 2024Quote: ... the cleared lysates were added to 1.5 mL glutathione-Sepharose 4B resin (17-0756-05, GE Healthcare) that had been pre-washed with buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2023Quote: ... cell lysate was circulated over a 5 mL HiTrap Chelating column (GE Healthcare, Cat# 17-0409-01) charged CoCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... GST fusion proteins were purified on glutathione-Sepharose 4 Fast Flow beads (GE Healthcare 17-5132-01). 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... The immune-complexes were collected with 50 ul of protein A sepharose (17-0780-01; GE Healthcare) at 4°C for two hours with rotation ...
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bioRxiv - Immunology 2023Quote: ... the cortex was homogenized and resuspended with 5 ml 70% Percoll Plus (GE Healthcare, 17-5445-02) and 5 ml 37% Percoll Plus placed on top ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a HiTrap TALON column and eluted with 50 mM sodium phosphate ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Recombinant protein was first purified on HisTrap HP column (GE Healthcare) using Buffer A (50mM Tris Base ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...