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Citations for GE Life Sciences :
451 - 500 of 2001 citations for Recombinant Human Interleukin 1 Beta since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... donkey anti-rabbit (#NA9340V, GE Healthcare, 1:4000). Membranes were washed after each incubation step three times for 5 min in TBS-T buffer (25 mM Tris/HCl pH 7.5 ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-rabbit (NA9340; GE Healthcare; 1:25,000). Protein extraction was performed using lysis Buffer (20 mM Tris (pH 8) ...
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bioRxiv - Immunology 2020Quote: ... and 1 ml Mono S column (GE Healthcare) as described (14) ...
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bioRxiv - Cancer Biology 2019Quote: ... goat anti-mouse (1:10,000; GE Healthcare NA931).
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bioRxiv - Cancer Biology 2019Quote: ... or anti-mouse-HRP (1:2000, GE Healthcare). Blots were imaged using a Syngene G-Box system ...
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bioRxiv - Cancer Biology 2019Quote: ... dilution 1:5000) were purchased from GE Healthcare.
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bioRxiv - Immunology 2021Quote: ... and 1% 100 × Penicillin-Streptomycin (GE Healthcare, #SV30010) at 37 °C / 5% CO2 ...
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bioRxiv - Physiology 2021Quote: ... or anti-mouse IgG (GE Healthcare; 1:5000) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 1 filter paper (GE Healthcare Life Sciences, USA), and the liquid fractions were concentrated with rotary evaporator to obtain the crude extracts [25 ...
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bioRxiv - Neuroscience 2021Quote: ... GST (goat, GE Healthcare, 27457701V, WB 1:5000), MYC (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... Monoclonal mouse-anti-His6 (1:1000, GE Healthcare) and monoclonal rabbit-anti-pT72-Rab8a (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... 1 µL of MabSelect resin (GE Healthcare, USA) pulldown mice serum after 14 days of immunization ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Rabbit secondary antibody (GE healthcare,1:10000). Membranes were developed by the Odyssey Fc Imaging system (LI-COR Bioscience).
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bioRxiv - Plant Biology 2022Quote: ... 1% dithiothreitol (all GE Healthcare, Piscataway, NJ, USA), 1 mM phenylmethanesulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2022Quote: ... containing Cy3-conjugated streptavidin (1:1000) (GE Healthcare). To probe expression of different molecular markers in labeled neurons ...
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bioRxiv - Microbiology 2022Quote: ... into BamHI/NotI-digested pGEX4-1 (GE Healthcare), or tagged with N-terminal TwinStrep (TS ...
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bioRxiv - Biochemistry 2023Quote: ... sheep anti-mouse (1:10,000; GE Healthcare #NXA931V) or donkey anti-rabbit (1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... and pLKO.1-shSIRT1.3 (GE Healthcare, Chicago, USA) plasmids as described previously.11 pLKO.1-Scrambled plasmids (Addgene ...
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bioRxiv - Biophysics 2024Quote: ... 1% Antibiotic Antimycotic Solution (GE Healthcare Life Sciences), and 0.5 mg/mL of the aminoglycoside antibiotic G418 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Whole venous blood collected in Citrate-Phosphate Dextrose-Adenine-1 (CPDA-1,JMS) and PBMC were isolated from whole blood over Ficoll-Paque PLUS (GE Healthcare) density gradient ...
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bioRxiv - Microbiology 2021Quote: ... Kinetic analysis was performed on each injection series using a 1:1 (Langmuir) binding model was applied using Biacore T200 Evaluation Software (GE Healthcare). Equilibrium dissociation constants (KD ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAi efficiency testing for ELAV was done from dissected central brains by Western blotting according to standard protocols as described 73 using a polyclonal rat anti-ELAV antibody generated against Drosophila ELAV (1:800) 45 and secondary HRP-coupled goat anti-rat antibody (1:5000, GE Healthcare) by chemoluminescence detection (Pierce ...
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bioRxiv - Immunology 2020Quote: ... Secondary antibody was incubated for 1 hour at room temperature (1:10000, sheep anti-mouse IgG-HRP or donkey anti-rabbit IgG-HRP, GE Healthcare). For development ...
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bioRxiv - Neuroscience 2019Quote: ... mouse monoclonal anti-tubulin antibody (1:1000, DSHB, University of Iowa, Iowa City, IA, USA) and secondary anti-mouse IgG-HRP (1:2000, GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... Kinetic constants were determined by fitting 1:1 Langmuir interaction model to the binding curves in the Biacore T200 Evaluation Software (GE Healthcare). Analysis of all A/P variants were restricted to concentrations of 25nM and lower ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Reduced proteins were now purified from the lysate samples using SP3 magnetic beads (1:1 mixture of SpeedBead Magnetic Carboxylate Modified Particles 65152105050250 and 45152105050250, GE Healthcare). To enable protein binding to the beads ...
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bioRxiv - Bioengineering 2020Quote: A solution of 25 mg/L SC-SWCNTs and 2 mg/mL C16-PEG2k-ceramide in 1% sodium cholate was added to a 1 kDa molecular weight cutoff dialysis cartridge (GE Healthcare). The solution was dialyzed against water with multiple water exchanges for a week ...
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bioRxiv - Microbiology 2021Quote: ... All sensorgrams were corrected by subtraction of the corresponding blank channel in addition to the buffer background and the kinetic constant determined using a 1:1 Langmuir model with the BIAevaluation software (GE Healthcare). Goodness of fit of the curve was evaluated by the Chi2 value with a value below 3 considered acceptable.
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bioRxiv - Neuroscience 2020Quote: ... and developed using an anti-rabbit HRP secondary antibody (1:5000, GE Lifesciences, NA931) and an anti-mouse HRP secondary antibody (1:10,000, GE Healthcare, NA935). Blots were imaged using an Amersham Imager 600 (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Data were fitted to a 1:1 interaction model using the evaluation software provided by the manufacturer (GE Healthcare Life Sciences). Reliability of the results was assessed according to U-value < 15 and χ2 < 5%Rmax ...
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bioRxiv - Immunology 2022Quote: ... The antibody and RBD affinity ability were estimated employing a 1:1 binding fit model in Biacore Evaluation software (GE Healthcare).
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bioRxiv - Biochemistry 2019Quote: ... the peak size-exclusion chromatography F86E RAD51 elution fraction was serially diluted (5, 1:1 serial dilutions) and loaded onto a Superdex200 10/300 GL size exclusion column (GE Healthcare) equilibrated with MALS Buffer (150 mM KCl ...
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bioRxiv - Immunology 2021Quote: All sensograms were corrected by subtraction of the corresponding blank channel and the kinetic constant determined using a 1:1 Langmuir model with the BIA evaluation software (GE Healthcare). Goodness of fit of the curve was evaluated by the Chi2 value with a value below 3 considered acceptable
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bioRxiv - Plant Biology 2019Quote: The construct of full length AtMLKL2 (residues 1-711) with N-terminal GST tag was cloned into the pGEX-6P-1 vector (GE Healthcare), and was expressed in Escherichia coli strain BL21(DE3 ...
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bioRxiv - Immunology 2019Quote: ... rVACV-eGFP-OVA-Early and rVACV-eGFP-OVA-Late were generated by transfecting plasmid DNA into BSC-1 cells infected with VACV-vRB21 at an MOI of 1 using the CellPhect Transfection Kit (GE Healthcare). The resulting rVACVs were plaque-purified 3× prior to characterization ...
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bioRxiv - Biochemistry 2021Quote: ... were flowed through with a concentration ranging from 0.05 to 100 nM and the data were fit to a 1:1 binding model using Biacore Evaluation Software (GE Healthcare).
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bioRxiv - Cell Biology 2020Quote: ... the corresponding cDNAs of mouse angulin-1 were amplified by PCR using KOD-Plus-Ver.2 DNA polymerase and subcloned into pGEX-6P-1 (GE Healthcare). To construct an expression vector for MBP-tagged N-ZO-1 (aa 1-862) ...
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bioRxiv - Microbiology 2021Quote: ... were used for immunoblotting along with sheep anti-mouse-HRP NXA931V secondary antibody (GE Healthcare, 1:5,000 or 1:10,000, respectively). All yeast growth and Western blot data shown are representative of at least two biological replicates.
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bioRxiv - Biochemistry 2021Quote: ... A 1:1 combination mix of two of magnetic carboxylate beads was used (Sera-Mag Speed beads, #45152105050250, #65152105050250, GE Healthcare). Beads were prepared fresh by rinsing with water three times prior to use at a stock concentration of 20 μg/μL ...
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bioRxiv - Microbiology 2022Quote: ... Ten µg of a 1:1 mixture of hydrophilic and hydrophobic carboxyl-coated paramagnetic beads (SeraMag, #24152105050250 and #44152105050250, GE Healthcare) were added for each µg of protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The binding affinity was determined by global fitting to a Langmuir 1:1 binding model within the Biacore Evaluation software (GE Healthcare).
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bioRxiv - Plant Biology 2022Quote: ... the gels were run in 1× RNA running buffer (1 × MOPS and 6% [v/v] formaldehyde) and blotted onto the Hybond-N+ membrane (GE Healthcare) soaked in 20× SSC (3.0 M NaCl and 0.3 sodium citrate) ...
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bioRxiv - Biophysics 2022Quote: ... 3 volumes of 1 mg/mL affinity-purified antibody was gently rotated with 1 volume Protein A Sepharose beads (GE Healthcare) overnight at 4°C or 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated for 1 h at room temperature with secondary antibody (anti-mouse IgG, 1:10,000 in 3% BSA/TBS-T; GE Healthcare, #RPN4201) linked to horseradish peroxidase ...
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bioRxiv - Cell Biology 2023Quote: ... three segments of MASTL (N: aa 1-340; M: aa 335-660; C: aa 656-887) were inserted into pGEX 4T-1(GE Healthcare). The resulting expression vectors were transfected into BL21 bacteria cells for protein expression and purification ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was diluted 1:1 with solubilization buffer without detergent and lysates were pre-cleared by incubation with Protein G sepharose beads (GE Healthcare) on a rotation wheel for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were washed three times with TBS-Tween 1 % and incubated for 1 h at room temperature with a secondary antibody anti-Mouse conjugated to horseradish peroxidase (GE Healthcare, NA9310 ...
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was finally concentrated to 1–10 mg mL−1 using centrifugal concentrators with a cut-off at 100 kDa (Vivaspin 100, GE Healthcare), frozen in liquid nitrogen and stored at −80 °C in storage buffer containing 50 mM Mes-KOH (pH 7) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mg lysates were incubated with the appropriate agarose-conjugated primary antibody for 3-4 h at 4°C or with unconjugated antibody (1-2 mg) overnight at 4°C followed by 1 h incubation with Protein G Sepharose beads (GE Healthcare). Immuno-complexes were washed four times with NETN buffer (20 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... to reach 120 mM of NaCl and were further passed through a Hi-Trap Q HP 1 mL column to remove residual DNA before binding on a Hi-Trap heparin HP 1 mL column (GE Healthcare) and elution with a NaCl gradient ...