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Citations for GE Life Sciences :
301 - 350 of 4258 citations for Recombinant Human IgG1Fc Protein Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The GST-tagged constructs were purified with glutathione beads (GE Healthcare), whereas ion metal affinity chromatography using Ni2+ beads (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... His-tagged MICAL1 was purified using HisTrap HP columns (GE Healthcare). Proteins were further purified using size exclusion chromatography and stored in a buffer of 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... GST-tagged Keap1 was purified on glutathione-Sepharose 4B (GE Healthcare), eluted with 10 mM reduced glutathione in 50 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... MBP tagged L7TIR was incubated with by PreScission protease (GE Healthcare) at 4°C or 22°C for 12 hr to remove the MBP tag ...
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bioRxiv - Plant Biology 2020Quote: ... The recombinant protein was purified from the cleared lysate using a combination of metal affinity chromatography using nickel-affinity (GE Healthcare, Chicago, IL, USA) and size exclusion chromatography using 16/600 Superdex 75 prep grade columns (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The GST-VH1 recombinant protein was purified by GST affinity chromatography and His-MBP-VH1 was purified by Ni-NTA affinity chromatography (GE Healthcare, Piscataway, NJ, USA). Purified proteins were dialyzed against Bis-Tris Buffer (50mM Bis-Tris ...
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bioRxiv - Immunology 2021Quote: ... Expression of the recombinant proteins was performed using ExpiCHO™ Expression system (Thermoscientific, USA, Cat# A29133) following purification using HisTrap™ (GE Healthcare, UK) and Strep-Tactin®XT (IBA ...
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bioRxiv - Immunology 2023Quote: Human ACE2 with Fc tag was immobilized onto Protein A sensor chips using a Biacore 8K (GE Healthcare). Purified SARS-CoV-2 mutants RBD were prepared in serial dilutions ...
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bioRxiv - Biochemistry 2019Quote: ... Biotinylated BET bromodomains were immobilised on a CAP chip (GE Healthcare) with a target density of ∼1000-1500 RU ...
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bioRxiv - Microbiology 2023Quote: ... biotinylated Affimers were immobilized on a Sensor Chip SA (GE Healthcare) through streptavidin-biotin interaction ...
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bioRxiv - Biochemistry 2023Quote: ... Purified biotinylated target was immobilized on a streptavidin chip (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... biotinylated RNA was streptavidin-captured on an SA-Chip (GE Healthcare) surface according to manufacturers instructions ...
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bioRxiv - Bioengineering 2021Quote: ... PEGylated mEGFP tagged ferritin was purified with PD10 desalting columns (GE Healthcare) equilibrated with 20mM HEPES ...
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bioRxiv - Microbiology 2020Quote: ... HisMBP-tagged PurR was loaded onto a HisTrap FF column (GE Healthcare) equilibrated with Lysis Buffer on an AktaPure FPLC (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged recombinant proteins were purified using nickel conjugated HisTrapTM Fastflow crude column (Cytiva, Marlborough, MA) with ÄKTA™ pure chromatography system (GE Healthcare Life Sciences, MA). Purified proteins were desalted with SephadexTM G-50 superfine resin (Cytiva ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were purified by loading the supernatant onto a 1 mL Protein A HP HiTrap column (GE Healthcare). Columns were equilibrated and washed using Dulbecco’s phosphate buffered saline (DPBS) ...
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bioRxiv - Microbiology 2020Quote: His-tagged 2019-nCoV S was immobilized to an NiNTA sensorchip (GE Healthcare) to a level of ∼800 response units (RUs ...
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bioRxiv - Neuroscience 2021Quote: ... Fc-tagged Nlgn1- ECD were purified by rProteinA Sepharose Fast Flow (GE Healthcare), eluted with 10mM glycine ...
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bioRxiv - Physiology 2022Quote: ... The biotinylated RS or heparin was immobilized to a streptavidin chip (GE Healthcare) based on the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... purified biotinylated ACE223 was immobilized on a streptavidin-coated (SA) chip (GE Healthcare) at ∼50 RUs ...
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bioRxiv - Cancer Biology 2023Quote: ... The biotinylated PCR products were immobilized on streptavidin-coated sepharose beads (GE Healthcare). DNA strands were separated using the PyroMark Q24 Vacuum Workstation ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated Omicron RBD ligand was immobilised on a SA sensor chip (GE Healthcare) at 3622 RUs ...
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bioRxiv - Biophysics 2020Quote: ... The GST was cleaved with GST-tagged HRV 3C protease (PreScission Protease, GE Healthcare). The GST and protease was removed with glutathione sepharose resin ...
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bioRxiv - Cell Biology 2023Quote: ... GST tagged VCP/p97 was affinity-purified with a GSTTrap FF column (GE Healthcare) with a flow rate of 1-5 ml/min on an ÄKTA purification system ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then incubated with GST-tagged PreScission protease (GE Healthcare GE27-0843-01) at a concentration of 50 µL of enzyme per 1 mL of bead slurry ...
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bioRxiv - Biochemistry 2024Quote: ... GST-tagged constructs were cloned into a modified form of pGEX4T-3 (GE Healthcare) with a TEV cleavage site and a second ribosomal binding and multiple cloning site (pABLO TEV ...
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bioRxiv - Immunology 2023Quote: Heavy and light chain variable regions were amplified from single-cell cDNA by a nested PCR approach as previously described.[33] The PCR products were cloned into expression vectors for production of full-length human IgG1 molecules in HEK293-F cells followed by purification of the secreted mAbs on Protein G columns (GE Healthcare). Sequence analysis was done with the IMGT/V-QUEST tool.[34]
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bioRxiv - Cell Biology 2019Quote: ... the samples were incubated with biotinylated sheep anti-mouse (1:200, RPN1001V, GE Healthcare) followed by peroxidase labeled streptavidin (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... biotinylated anti-chicken (BA9010, 1:250, Vector Laboratories, CY3-streptavidin (1:500, GE Healthcare). Cell nuclei were counterstained with DAPI (0.2 mg/ml in PBS ...
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bioRxiv - Microbiology 2021Quote: Biotinylated RNA oligonucleotides were immobilised onto the surface of streptavidin-coated chips (GE Healthcare) in a BIAcore T200 at a concentration of 100 nM ...
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bioRxiv - Biochemistry 2019Quote: ... The His-tagged UBC13 and its variants were bound to HisTrap HP columns (GE Healthcare) and were eluted with 100mM-500mM Imidazole gradient ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged Ola1p was purified via IMAC using an ÄKTA Pure FPLC system (GE Healthcare) equipped with a Ni-NTA column (1 ml HisTrap HP column ...
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bioRxiv - Biochemistry 2021Quote: ... The His-tagged ZO-1-PDZ2 were purified using a HiTrap Chelating HP (GE Healthcare), where a renaturation gradient by exchange of denaturation buffer to a gel filtration buffer (Tris 50 mM (pH 7.5) ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the his-tagged FABP1 was purified using HisTrap HP affinity column (GE Healthcare, Chicago, IL), the tag was cleaved by thrombin and the cleaved protein was purified and buffer exchanged by gel filtration into 10 mM potassium phosphate pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies were added as follows: biotinylated anti-mouse 1:200 (GE Healthcare RPN 1001) followed by Tyramide Super Boost with Alexa Fluor 488 1:500 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... Bound biotinylated peptide was detected by addition of streptavidin-alkaline phosphatase conjugate (GE Healthcare, France) and 4-methylumbelliferyl phosphate substrate (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... biotinylated Wuhan RBD ligand was immobilised on a streptavidin-coated (SA) sensor chip (GE Healthcare) at 2453 RU ...
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged products were then purified using Glutathione Sepharose 4B according to manufacturer’s instruction (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... GST-tagged PfHDAC1 was purified from the soluble fraction with glutathione Sepharose 4B beads (GE Healthcare) using 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2021Quote: ... or GFP tagged INO1 were acquired on a DeltaVision Elite imaging system (GE Healthcare Life Sciences) with a 60x/1.42 NA oil ...
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Biochemistry 2022Quote: Purified GST-tagged DIDATRX and DIDATRX fragments were immobilized on Glutathione sepharose 4B beads (GE Healthcare) under saturating conditions in binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Bound antibodies were detected by applying biotinylated goat anti-rabbit IgG (GE Healthcare, Velizy Villacoublay, France) for 2h at room temperature ...