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Citations for GE Life Sciences :
301 - 350 of 1208 citations for Recombinant Human IGF2BP2 His & GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... A Hi-Trap® desalting column (GE Healthcare), equilibrated with 20 mM sodium phosphate buffer pH 7.4 ...
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bioRxiv - Biochemistry 2022Quote: ... His-tag was cleaved by thrombin (GE Healthcare) and NaCl concentration was reduced to 100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... and purified using His-Trap HP (GE Healthcare) followed by gel filtration using HiLoad 16/60 Superdex 75 columns on an ÄKTA Xpress ...
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bioRxiv - Biophysics 2024Quote: ... loaded onto a His-Trap column (GE Healthcare), and eluted by applying a linear imidazole gradient from 20 mM to 200 mM over 30 column volumes ...
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bioRxiv - Plant Biology 2024Quote: ... purified on a His-Trap column (GE Healthcare) following the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2020Quote: ... freshly purified GST-RILP protein bound to GSH-Sepharose 4B beads (GE Healthcare) was incubated with extracts from transfected HEK293T cells (one 10-cm diameter dish per assay ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins were pull-down with glutathione Sepharose 4B beads (GE Healthcare), pre-washed with ice-cold PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Glutathione sepharose 4B GST beads was purchased from GE Healthcare (Mississauga, ON, Canada). Lipofectamine 2000 and Alexa Fluor 488 goat anti-mouse IgG were purchased from Invitrogen (Burlington ...
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bioRxiv - Cell Biology 2020Quote: ... GST tag was removed by incubating with 2 units PreScission protease (GE Healthcare) per 100 μg protein for 16-18 h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The GST fusion proteins were purified with Glutathione Sepharose ™4B (GE Healthcare) according to the manufacturer’s protocol with Binding Buffer I [50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... GST tags were cleaved with PreScission protease per the manufacturer’s instructions (GE Healthcare). Isolated CFP1 fragments were dialyzed in 25% glycerol ...
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bioRxiv - Plant Biology 2019Quote: ... The GST-VCA fusion protein associated with the glutathione-agarose 4B (GE Healthcare) was incubated with the Arp2/3 complex in pull-down buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Primary antibodies were directed against GST (GE Healthcare Cat# 27-4577-01, RRID:AB_771432), murine AOC3 (Abcam Cat# ab42885 ...
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bioRxiv - Developmental Biology 2020Quote: ... Cell lysates were precleared with GST immobilized on glutathione–Sepharose 4B (GE Healthcare) and then incubated with the indicated GST fusion proteins (2–5 μg ...
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bioRxiv - Molecular Biology 2021Quote: ... The GST-proteins were purified using Glutathione Sepharose 4B (GE Healthcare Life Sciences) according to the manufacturer’s protocol.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The GST-TTD fusion protein was purified using Glutathione sepharose 4B (GE Healthcare) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The GST tag was cleaved by the addition of PreScission Protease (GE Healthcare) with rocking incubation overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... GST-β2HA was purified from the soluble fraction using glutathione resin (GE Healthcare). The GST tag was removed by overnight cleavage at 4 °C using GST fusion 3C protease (Prescission ...
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bioRxiv - Cell Biology 2020Quote: ... the GST-tag was removed by proteolytic cleavage with PreScission Protease (GE Healthcare), followed by a final polishing step of the proteins by size-exclusion chromatography on an Äkta Purifier System using either HiLoad 26/600 Superdex 200 or HiLoad 26/75 Superdex columns (GE Healthcare) ...
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bioRxiv - Cell Biology 2021Quote: ... GST-fusion proteins were purified by incubation with glutathione-Sepharose beads(GE Healthcare). The precipitates were washed 3X with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... GST fusion proteins were immuno-precipitated using Glutathione Sepharose 4B beads (GE Healthcare). Fusion protein-bound beads were used directly in GST pull-down assays ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... GST-RIG-I protein variants were affinity purified by GSTrap column (GE Healthcare). After sample loading ...
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bioRxiv - Biophysics 2023Quote: ... The clarified supernatants were loaded onto a GST column (GSTrap HP, GE Healthcare) or a nickel affinity chromatography column (HiTrap HP ...
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bioRxiv - Plant Biology 2024Quote: ... GST-TZF1 was purified using glutathione-Sepharose 4B resin (GE Healthcare Bio Sciences) as previously described (Qu et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... GST-FBH1[1-250] in the pGEX6P-1 vector (Amersham/GE Healthcare/Cytiva) was introduced into BL21:DE3 Rosetta cells (Novagen) ...
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bioRxiv - Plant Biology 2023Quote: ... GST fusion proteins were purified on Glutathione-Sepharose® 4B beads (GE Healthcare) and eluted using a solution of 50LmM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2019Quote: ... fractions containing PGDH-His or RBBP9-His were concentrated and loaded onto a gel filtration column (Superdex 200, 10/300 GL, GE Healthcare) pre-equilibrated with 20mM sodium dihydrogen phosphate pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... NaCl concentration was readjusted to 500 mM by spiking with 5M NaCl stock solution and protein solution was dialyzed overnight into SEC1 buffer at 4°C followed by protein separation from His tag on a His-Trap column (GE Healthcare), concentration with an Amicon Ultra 15 MWCO10kDa (Millipore ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: SARS-CoV-2 PLpro-His (PLpro-His) protein was concentrated and loaded to a HiLoad 16/60 Superdex 75 size exclusion column (GE Healthcare) pre-equilibriated with 20 mM Tris pH 8.0 and 5 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... the His-MBP and His tags were removed by TEV protease and a passage through a Ni2+chelating Sepharose (GE Healthcare). The final purification step was size-exclusion chromatography on Superdex 200 size exclusion chromatography (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: His-SUMO-SEAL and His-SUMO-SEALGGG were analyzed by size-exclusion chromatography (SEC) on a Superdex S75 column (GE Healthcare) in buffer containing 20 mM of HEPES-NaOH ...
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bioRxiv - Molecular Biology 2021Quote: ... loaded onto 5 ml His-FFTrap column (GE Healthcare), equilibrated in His-A buffer (50 mM HEPES/NaOH pH 8.5 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and ion-exchange (Hi-Load Q column, GE Healthcare) for the 5FW-labeled protein ...
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bioRxiv - Microbiology 2020Quote: ... were transferred onto a His SpinTrap column (GE Healthcare). The purified fractions were subjected to desalting and concentration using an Amicon Ultra centrifugal filter unit (30 kDa cutoff ...
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bioRxiv - Molecular Biology 2019Quote: ... or Ni-NTA-Agarose (GE healthcare, His-B-MYB). Lysates of HeLa cells expressing HA-tagged B-MYB constructs were incubated with 5 mg immobilized GST or GST-WW1/2-YAP for 3 hours at 4°C ...
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bioRxiv - Immunology 2021Quote: ... functionalised with an anti-His capture kit (GE Healthcare) using a Biacore T200 instrument ...
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bioRxiv - Cancer Biology 2021Quote: ... and the GST-fusion protein was purified using Glutathione-Sepharose affinity columns (GE Healthcare). The proteins were finally eluted in PBS containing 20 mM glutathione (pH 8 ...
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bioRxiv - Molecular Biology 2021Quote: ... GST-OsbZIP86 and MBP-OsSAPK10 were incubated with Glutathione Sepharose 4B (GE Healthcare, USA) in pull-down buffer (50 mM Tris-HCl ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... GST-fusion proteins were purified using Glutathione Sepharose 4 Fast Flow beads (GE Healthcare), washed in lysis buffer and kept on beads in lysis buffer with 1 mM DTT at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and GST-hFMRP KH0-KH1-KH2 were purified using glutathione affinity chromatography (GE Healthcare). All proteins were further purified using Superdex 75 16/60 or Superdex 200 16/60 (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... The GST affinity tag was then cleaved using the PreScission Protease (GE Life Sciences) for four hours in cleavage buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... GST fusion proteins were purified over Glutathione (GSH) beads (GE Healthcare Biosciences, Pittsburg, PA) in PBS buffer supplemented with protease inhibitors and eluted with 20mM GSH in PBS pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... The GST-CP was bound to glutathione Sepharose beads (GE Healthcare, 17- 5132-01) for 3 h at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare) for 1 hour at 4 °C with gentle rocking ...
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bioRxiv - Cell Biology 2021Quote: ... and cutting of the GST tag from purified proteins using PreScission protease (GE Healthcare) in 150 mM NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... cell lysates of these complexes were firstly treated with GST agarose beads (GE Healthcare). And then the protein-bound beads were transferred into gravity columns and treated with 3C protease overnight at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Peptides were purified via GST affinity chromatography by FPLC (Äkta Prime Plus, GE Healthcare). The GST tag was proteolytically cleaved by TEV-protease digestion ...
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bioRxiv - Cell Biology 2019Quote: ... The chip was prepared by covalent coupling of an anti-GST antibody (GE Healthcare) as capture molecule to the dextran surface of both flow cells of a CM5 Chip (GE Heathcare ...
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Receptor-like role for PQLC2 amino acid transporter in the lysosomal sensing of cationic amino acidsbioRxiv - Cell Biology 2020Quote: ... and the GST-WDR41 fusion was generated in pGEX-6T-1 (GE Life Sciences). These new plasmids were generated by Gibson Assembly or Q5 Site-Directed Mutagenesis according to manufacturer instructions (New England Biolabs) ...
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bioRxiv - Plant Biology 2021Quote: ... using the GST gene fusion system for heterologous expression and affinity purification (GE Healthcare). DNA templates for the cystatins were synthesized as g-blocks (IDT ...