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Citations for GE Life Sciences :
151 - 200 of 4666 citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: GST-tagged proteins were purified using affinity chromatography with Glutathione Sepharose beads (GE Healthcare) in a cold room ...
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bioRxiv - Biophysics 2020Quote: ... 6xHis-tagged PPARγ LBD protein was immobilized on an NTA sensor chip (GE Healthcare), which includes a reference flow cell for background subtraction ...
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bioRxiv - Microbiology 2022Quote: GST-tagged protein was purified using Glutathione-based affinity chromatography (GSTrap FF GE Healthcare). Cells were thawed on ice and resuspended in Buffer F (20 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... The GST-tagged proteins were absorbed with Glutathione-Sepharose 4B affinity matrix (GE Healthcare) for 1.5hrs and recovered by 3C protease cleavage at 4°C overnight in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged proteins were purified with Glutathione Sepharose 4B (GE Healthcare, GE17-0756-01), washed and either eluted with glutathione of cleaved with PreScission protease (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... All poly-histidine tagged proteins were purified using the Ni-NTA agarose (GE Healthcare), and eluted with imidazole ...
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bioRxiv - Neuroscience 2019Quote: ... GST-tagged proteins were captured by incubation with 20 μl glutathione sepharose (GE Healthcare) beads for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: For SPR: MBP-tagged proteins were immobilised on Sensor chip CM5 (GE Healthcare, #BR100012) and purified untagged RAC1 Q61L was titrated at different concentrations and the response signals were recorded and fitted into a 1:1 binding model ...
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bioRxiv - Plant Biology 2023Quote: ... Tagged proteins were purified separately using Glutathione Sepharose 4B (17075601, GE Healthcare, Chicago, USA) or Ni-NTA affinity agarose beads (30210 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants of GST-tagged protein homogenates were loaded onto glutathione SepharoseTM beads (GE healthcare). Prior to the elution ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus MBP tag was removed by TEV protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant expressed protein was purified using a HisTrap column (GE Healthcare). The hexahistidine plus yeast sumo tag was removed by ulp1 protease digestion followed by a second step HisTrap column (GE Healthcare) ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant G6b-B proteins were immobilized on CM5 sensor chips (GE Healthcare) via an Fc antibody using the Human Antibody Capture Kit (GE Healthcare) ...
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bioRxiv - Immunology 2019Quote: ... Recombinant antibodies were purified from supernatants using protein-G beads (GE Healthcare).
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange on a HiPrepTM 26/10 desalting column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant protein was then isolated using Ni-NTA resin (GE healthcare) and eluted with a buffer containing 25 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant proteins were purified on a HiTrap SP HP column (GE Healthcare) and subsequently on a HiTrap Q HP column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... recombinant proteins were first purified using a Ni-NTA column (GE HealthCare) and the His-GST tag was removed by cleavage with 3C-PreScision protease and subsequent incubation with Ni2+ beads to remove the uncleaved protein and the tag from the solution ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...
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bioRxiv - Microbiology 2022Quote: ... and the obtained supernatant was purified using 0.22 μm filter and passed over a Strep-Tactin II column (GE Healthcare) to collect secreted TraC ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...
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bioRxiv - Plant Biology 2020Quote: ... tNPR1-His proteins were further purified by HiLoad 16/600 Superdex 200 (GE Healthcare) with 200 mM NaHCO3 ...
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bioRxiv - Neuroscience 2022Quote: ... together with a 5 ml HisTrap HP His tag protein purification column (GE Healthcare). The supernatant was loaded onto the column initially washed with 25 ml buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant fraction containing proteins were loaded on 5ml His-Trap column (GE Healthcare) and eluted with a linear gradient of elution buffer containing 40mM to 500mM Imidazole ...
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bioRxiv - Cell Biology 2021Quote: ... His-tag fusion proteins were purified from lysates using HisTrap HP column (GE Healthcare). The size and purity of the protein preparations were verified by SDS-PAGE and Coomassie Blue staining ...
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bioRxiv - Biochemistry 2022Quote: ... and His(6)GFP –AnkBD proteins were purified using Glutathione Sepharose 4B (GE Healthcare) or Talon® (Clontech ...
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bioRxiv - Microbiology 2022Quote: ... The purified protein was desalted using a His Trap Desalting columns from GE Healthcare according to the prescribed protocol and was stored at −80°C with 40 % glycerol.
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were purified over 1-2 ml His-Trap FF columns (GE Healthcare) with elution buffer containing 250 mM Imidazole ...
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bioRxiv - Plant Biology 2022Quote: ... The His-MDIS1CD protein is loaded onto a Ni-NTA agarose column (GE Healthcare) and eluted using elution buffer containing 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... coli as His-tag proteins and were purified using the IMAC principle (GE healthcare). Following purification ...
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bioRxiv - Molecular Biology 2019Quote: ... were supplemented with 20 mM imidazole to reduce nonspecific binding of unwanted protein to the HisTrap HP histidine-tagged protein purification columns (GE Healthcare). In brief ...
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bioRxiv - Neuroscience 2023Quote: ... Biotinylated proteins were isolated using streptavidin magnetic sepharose beads (GE Healthcare Cat# 28-9857-38) overnight at 4°C and then washed seven times in RIPA buffer ...
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bioRxiv - Biochemistry 2019Quote: ... Recombinant proteins were routinely purified by fast protein liquid chromatography (FPLC) using an AKTA purifier system (GE Healthcare), eluting with a linear gradient of 25 mM Tris-HCl pH 7.4 ...
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bioRxiv - Physiology 2023Quote: ... Recombinant proteins were purified using Hitrap Protein A HP column on an ÄKTA pure chromatography system (GE healthcare) and eluted with 0.1M Glycine pH 3.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted protein was separated on a 5 ml HiTrap STREP column (GE Healthcare, Little Chalfont, UK), and eluted with 2 mM desthiobiotin ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... the recombinant protein was purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Heparin HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... The recombinant proteins were purified through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was further purified through 5 mL HiTrap Q HP column (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant proteins were first purified on a HisTrap HP column (GE Healthcare), followed by buffer exchange using HiPrep 26/10 desalting column (GE Healthcare).
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bioRxiv - Cell Biology 2022Quote: ... The recombinant proteins were purified by Ni+ sepharose 6 fast flow (GE Healthcare), glutathione Sepharose 4B (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant tau proteins were immobilized on the CM5 sensor chip (Biacore, GE Healthcare) via amine coupling ...
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bioRxiv - Immunology 2021Quote: ... Recombinant IgG was purified using HiTrap Protein A columns (GE Healthcare Life Sciences). For IgA1 or IgA2 antibodies ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The recombinant protein was purified through a nickel-nitrilotriacetic acid column (GE Healthcare) and subsequently loaded on a gel filtration column Sephacryl S-200 HR (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Purified recombinant proteins were loaded onto Superdex 75 10/300 column (GE Healthcare) in 150mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... The recombinant proteins were purified by Ni2+-IMAC on HisTrap columns (GE Healthcare). Fractions ...
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bioRxiv - Immunology 2020Quote: ... The recombinant GST fusion proteins were affinity purified on GSH-Sepharose (GE Healthcare) from bacterial cultures incubated with 0.25 mM isopropyl-β-D-thiogalactopyranoside (Sigma-Aldrich ...