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Citations for GE Life Sciences :
251 - 300 of 2580 citations for Recombinant Human Endothelin Converting Enzyme 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... was purified using an ÄKTAprime plus fast protein liquid chromatography (FPLC) system equipped with a 1-mL His-Trap HP column (GE Healthcare-Amersham Biosciences) and the high salt buffer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was filtered using 0.45 micrometer membranes (PES, ClearLine) and the protein purification was carried out by IMAC using 1 mL His-Trap FF columns (GE Healthcare Life Sciences) and a NGC chromatography system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... one of which was heating in a 65 °C water bath for 60 minutes and the second was using a Hi Trap QHP column (1 mL GE Healthcare, United States). When loading ...
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bioRxiv - Microbiology 2022Quote: ... cleavage was achieved after elution of GbpA from the column by adding 1 unit of PreScission protease enzyme and incubation overnight at 4°C per the instructions of the manufacturer (GE Healthcare).
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bioRxiv - Plant Biology 2020Quote: ... DNA samples digested with restriction enzymes were separated in 1% agarose gels and blotted onto Hybond-XL nylon membranes (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The second PCR joined these two spliced fragments to generate the PCR product encoding the H172Y mutated Mpro including the cleavage sites of the restriction enzymes for cloning into the vector PGEX-6p-1 (GE Healthcare). The amplified PCR product was digested with BamHI and XhoI and ligated into the vector PGEX-6p-1 digested with the same restriction enzymes ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNAs encoding human NGN3 protein fragments were sub-cloned into pGEX4T-1 (GE Healthcare), expressed in Escherichia coli ...
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bioRxiv - Molecular Biology 2021Quote: ... 100 μl purified recombinant CPF at 1 mg/ml was loaded onto a Superdex 200 10/300 GL increase column (GE Healthcare) pre-equilibrated with 50 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... the supernatant was collected for purification using His Trap Excel column (GE Healthcare). The proteins were labeled with an AlexaFluor dye through the ybbR-tag and gel-filtered via a Superdex 75 10/300 GL column ...
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bioRxiv - Cell Biology 2019Quote: ... His-NF1 GAP was purified using a HisTrap excel-affinity column (GE Healthcare) and eluted in buffer containing 50mM Tris ...
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bioRxiv - Neuroscience 2020Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was loaded on a Ni-NTA His-trap column (GE healthcare), washed with 10 column volume of buffer A containing 30 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were loaded on Hi-trap Q HP (GE Healthcare, cat.17115401) anion exchange column ...
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bioRxiv - Cell Biology 2020Quote: MCAK was first affinity purified by binding to His-Trap HP (GE Healthcare) in binding buffer (50mM NaPO4 ...
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bioRxiv - Molecular Biology 2020Quote: ... and lysates were cycled over a 5 ml His-Trap column (GE Healthcare) at 4°C overnight ...
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bioRxiv - Biophysics 2019Quote: ... further loaded onto a His-Trap HP column (GE Healthcare, Chicago, IL, USA), washed and then eluted in gradient with the same buffer supplemented with 250 mM imidazole pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The supernatant was purified by His Trap™ FF crude column (GE Healthcare) which was eluted first by 50 mM imidazole (Innochem) ...
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bioRxiv - Microbiology 2021Quote: ... The sera were purified on Hi-Trap Protein G HP columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and a Hi-trap Q HP anion exchange column (GE Healthcare Life Sciences). Fractions containing α-syn were identified by SDS-PAGE and Coomassie staining ...
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bioRxiv - Biochemistry 2019Quote: ... The dialyzed protein extract was purified via a His-trap column (GE Healthcare) previously equilibrated with 20 mM sodium phosphate buffer ...
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bioRxiv - Biochemistry 2021Quote: ... RNase HI was loaded onto a 16/600 Superdex 75 column (GE Healthcare), whilst PolI and Ligase were further purified on a 16/600 Superdex 200 column (GE healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded onto a 3ml His SpinTrap™ column (GE Healthcare) previously charged with Ni2+ and equilibrated in lysis buffer ...
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bioRxiv - Immunology 2020Quote: ... lysate was affinity purified using Cobalt-charged His-Trap Chelating column (GE Healthcare) through imidazole gradient elution ...
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bioRxiv - Immunology 2020Quote: ... Harvested supernatants were applied to a Hi-Trap Nickel excel columns (GE Healthcare) using an Akta pure (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ... Nbs from PE were purified using IMAC Hi-Trap columns (GE Healthcare, 17092003) following manufacturer’s instructions and eluted using 300 nM Imidazole ...
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bioRxiv - Microbiology 2023Quote: ... The F341_RBP was purified using Ni2+- NTA-affinity chromatography (His GraviTrap, GE Healthcare) according to manufacturer’s instructions and exchanged into PBS buffer using 10 kDA Amicon® Ultra-15 Centrifugal filter units ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was applied to a 5 ml His-TRAP column (GE Healthcare) and eluted with a gradient of 20 mM to 500 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... HIS-p300 was further purified on a Q sepharose column (HiTrap, GE Healthcare) in BC buffer and eluted between 100-600 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was loaded onto a nickel-chelated Hi-trap column (GE Healthcare) and eluted with a linear gradient of 25-500 mM imidazole in 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2024Quote: ... The NRP2-his was purified using a HiTrap IMAC FF Ni2+ (GE Healthcare) affinity column and Superdex 75 10/300 size exclusion chromatography (GE Healthcare ...
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bioRxiv - Plant Biology 2019Quote: ... The fusion protein was isolated from soluble cell lysate by Co-NTA affinity chromatography with two His-Trap 1 mL TALON Crude columns (GE Healthcare Life Sciences, 28953766). Chromatography buffers contained sodium phosphate 20 mM pH 8.0 ...
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bioRxiv - Genomics 2020Quote: ... The His6-tagged fusion protein was purified with a Ni2+-nitrilotriacetic acid column (GE Healthcare). The EtHAS B protein ...
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bioRxiv - Cell Biology 2021Quote: ... The tagged Ola1p was purified via IMAC using an ÄKTA Pure FPLC system (GE Healthcare) equipped with a Ni-NTA column (1 ml HisTrap HP column ...
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bioRxiv - Microbiology 2022Quote: ... Hexa-histidine tagged protein was purified using Nickle-based affinity chromatography (HisTrap HP, GE Healthcare) on Akta prime FPLC system ...
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bioRxiv - Biochemistry 2023Quote: ... StrepII-tagged Cas1-Cas2/3 complexes were affinity purified on StrepTrap HP resin (GE Healthcare) and eluted with Cas1-2/3 Lysis Buffer containing 3 mM desthiobiotin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2021Quote: ... Recombinant proteins were purified with a HisTrapTM HP (GE Healthcare, Sweden) by an AKTA pure system (GE Healthcare ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant RBD was captured by Ni-NTA resin (GE Healthcare) and eluted with 500 mM imidazole in HBS buffer ...
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bioRxiv - Plant Biology 2021Quote: ... The recombinant expressed protein was purified using HisTrap column (GE Healthcare). The His-Sumo tag was digested by ulp1 protease and removed by a second HisTrap column ...
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bioRxiv - Molecular Biology 2019Quote: ... Recombinant protein was purified using HiTrap DEAEFF (GE Healthcare, Chicago IL), HisTrapHP (Ni-NTA ...
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bioRxiv - Biochemistry 2020Quote: ... Recombinant proteins in 10 mM acetate buffer (pH 4.5, GE Healthcare) was then injected over the chip surface at a flow rate of 30 μl/min to couple the amino groups of the recombinant proteins to the carboxymethyl dextran matrix ...
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bioRxiv - Molecular Biology 2021Quote: All recombinant proteins were purified firstly by Ni-NTA (GE healthcare). TOFU-6/PICS-1 was further purified by HiLoad 16/600 Superdex 75 column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a GSTrap column and elution with 50 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant proteins were purified on an AKTA Purifier system (GE Healthcare) using a HiTrap TALON column and eluted with 50 mM sodium phosphate ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Recombinant protein was first purified on HisTrap HP column (GE Healthcare) using Buffer A (50mM Tris Base ...
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bioRxiv - Cell Biology 2022Quote: Recombinant GST-NF2 was bound to a GST resin (GE Healthcare), and MBP-NF2 was bound to an MBP resin (New England Biolabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... recombinant proteins were purified using Ni-Sepharose affinity chromatography (GE Healthcare). Final elution was carried out at 250 mM imidazole ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant proteins were purified by glutathione Sepharose 4B (GE Healthcare) or Amylose Resin High Flow (New England Biolabs ...
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bioRxiv - Genetics 2023Quote: ... was incubated with recombinant GST-DOCK3-PXXP or GST alone plasmids (constructs cloned into pGEX-6P-1 plasmid; GE Healthcare; Chicago, IL) in GST reaction buffer (250 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Biophysics 2019Quote: ... the protein was further purified using ion-exchange Hi-Trap Q HP-(GE Healthcare). The protein quality was assessed by SDS-PAGE and analytical size-exclusion chromatography using the Superpose 10/300 column (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... The dialyzed protein was then passed through a Hi-Trap Chelating column (GE Healthcare) and the unbound material was collected ...