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Citations for GE Life Sciences :
551 - 600 of 4432 citations for Recombinant Human EGFR protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... The proteins were purified using HiTrap protein A column (GE Healthcare, 17040303).
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bioRxiv - Molecular Biology 2023Quote: ... 1,000 μg of proteins were prepared and pre-cleared with 20 μl of protein A-Sepharose and protein G-Sepharose beads mixture (GE Healthcare). 2 μl of (HA ...
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bioRxiv - Cell Biology 2021Quote: ... of SARS-CoV-2: the sequence encoding the N-terminally GST-tagged cytoplasmic domain of S were cloned into the vector pGEX6p2 (GE Healthcare Life Sciences). For GST-S (1255-1273 ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged Cg1978-C-His to GyrA-N-Strep was analyzed by SPR analysis in a Biacore 3000 device (GE Healthcare, Freiburg, Germany) using a Sensor Chip CM5 (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... and 6xHis-SMT3/SUMO tagged IMPDH2 was initially purified by Ni affinity chromatography using either a HisTrap FF column (GE Healthcare Life Sciences) on an Äkta Start chromatography system or a handpacked HisPur™ Ni-NTA resin (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then hybridized with 32P-labeled oligonucleotide probes using the Rapid-hyb buffer (GE Healthcare). Radioactive membranes were exposed to PhosphorImager screens and signals were revealed using Typhoon imager (GE Healthcare) ...
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bioRxiv - Plant Biology 2020Quote: ... α [32P] dCTP-labeled probes were generated by random priming (Megaprime DNA labeling kit; GE Healthcare). Hybridization was performed in Church Buffer (1% [w/v] BSA ...
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bioRxiv - Cell Biology 2021Quote: ... The fluorescently labeled membrane was imaged by the Typhoon FLA 9500 scanner (GE Healthcare Life Sciences) per the manufacturer’s protocol.
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bioRxiv - Biophysics 2020Quote: ... 6-FAM labeled RNA products were visualized by Typhoon 95000 FLA Imager (GE Healthcare Life Sciences).
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bioRxiv - Immunology 2020Quote: ... The washed membrane was incubated with a secondary antibody labeled with horseradish peroxidase (HRP) (GE healthcare) for 1 hour at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The fluorescently-labeled mAb conjugates were purified via size exclusion chromatography (PD-10 column; GE Healthcare) and concentrated using 50 KDa cutoff amicon filters.
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bioRxiv - Molecular Biology 2022Quote: ... Human cDNA of JAZF1 was purchased from GE Healthcare. Open reading frame (ORF ...
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bioRxiv - Immunology 2021Quote: Human PBMCs were separated on Ficoll-Paque (GE Healthcare) by density centrifugation of heparinized blood from healthy donors (“Etablissement Français du Sang” EFS ...
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bioRxiv - Developmental Biology 2019Quote: ... 1ml supernatant (2mg protein) was incubated with 30μl Protein G Sepharose (GE Healthcare) for two hours on a rotator at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were pre-cleared using 40 μl of Protein G Sepharose (GE Healthcare), for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Soluble Ig-fused proteins were purified using protein A-Sepharose beads (GE Healthcare). Bound proteins were eluted with 0.1 M glycine (pH 2.5 ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were further incubated with protein A/G-sepharose beads (GE Healthcare) for 2 hours ...
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bioRxiv - Immunology 2022Quote: ... fusion protein was purified from cell supernatant using protein A column (GE healthcare). After digestion with TEV protease ...
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bioRxiv - Immunology 2021Quote: Purified recombinant antibodies were assessed by SDS-PAGE under nonreducing conditions using the Amersham Phastsystem™ (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant rhodopsin was purified by affinity chromatography (HisTrap™ FF Crude column, GE Healthcare Life Science, Chicago, IL) and gel filtration (HiPrep™ 26/10 desalting ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant Sau-MenD was purified at room temperature by IMAC with 5 mL HisTrap HP columns (GE Healthcare) and an imidazole gradient of 20–500 mM over 50 mL ...
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bioRxiv - Immunology 2023Quote: ... Recombinant HA molecules were purified from these culture supernatants by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with imidazole gradient ...
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bioRxiv - Cancer Biology 2022Quote: Human peripheral blood mononuclear cells (PBMC) were purified from human blood buffy coat using Ficoll Paque Plus (GE Healthcare, cat. 17-1440-02) reagent and CD14+ microbeads (MACS Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Recombinant 6xHis-tagged mouse GRP78 was expressed in E coli BL21 cells and purified on a HisTrap™ HP Nickel column (GE healthcare, Uppsala, Sweden). Twenty micrograms (0.3 nmol ...
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bioRxiv - Molecular Biology 2020Quote: ... Gels were stained with Coomassie Blue (Phast Gel ™ Blue R, GE Healthcare, Sweden).
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bioRxiv - Cell Biology 2022Quote: The real time protein-protein interaction of protease and PDZ domain proteins of PfHtrA2was analysed by SPR using Biacore T200 instrument (GE Healthcare, Uppsala, Sweden) as described previously [7] ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated in horseradish peroxidase-labeled goat anti-rabbit or goat anti-mouse antibodies (GE Healthcare; 1:10,000) for 1 h at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Labeled probes were cleaned up with MicoSpin S-200 HR columns (GE Healthcare Life Science, Little Chalfont) according to the manufacturer’s manual.
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bioRxiv - Biophysics 2020Quote: ... The labeled viruses were purified from excess dyes on a Nap-5 gel filtration column (GE Healthcare) that was equilibrated with 50 mM HEPES ...
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bioRxiv - Biophysics 2021Quote: ... The dual-labeled heterodimer was then purified by cation exchange chromatography (Mono S 10/100, GE Healthcare) in 25 mM Tris pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Excess P32 gamma-ATP was then separated from labeled AAF DNA by G50 spin column (GE Healthcare). Labeled AAF DNA was mixed 1:4 with unlabeled AAF DNA prior to use for electromobility shift assays (EMSA).
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bioRxiv - Molecular Biology 2019Quote: ... was labeled with dCTP α-32P using Amersham Rediprime II DNA Labelling kit (GE Healthcare, Buckinghamshire, England) and used for hybridization at 42 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit; GE Healthcare). Hybridizations were performed at 65°C in Rapid-Hyb buffer (GE Healthcare ...
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bioRxiv - Biophysics 2021Quote: ... H2A.Z(K120C)-H2B-3xFlag and Swc7 were all cysteine-labeled with maleimide-functionalized Cy3 dye (GE Healthcare) using previously published protocols (53) ...
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bioRxiv - Biochemistry 2022Quote: ... Radioactive labeled mitochondrial translation products were detected using Phosphor screens and Amersham Typhoon imaging system (GE Healthcare).
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bioRxiv - Microbiology 2023Quote: ... Biotin-labeled SARS CoV2 5’UTR RNA was immobilized on streptavidin-coated sensor chips (GE Healthcare Lifescience) to a final concentration of 300 Resonance Units (RU)/flow cell ...
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bioRxiv - Microbiology 2023Quote: ... a probe was labeled using the Amersham Gene Images AlkPhos Direct Labelling and Detection System (GE Healthcare) according to the manufacturer’s protocol with a DNA fragment generated by PCR using primer pair S01/S02 (Supp ...
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bioRxiv - Microbiology 2023Quote: ... Cy5-labeled cDNAs were detected by fluorescent mode using a Typhoon FLA 9500 (GE Healthcare Life Sciences) Gel Scanner and LPR Ch.2 filter and 635 nm laser.
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bioRxiv - Biochemistry 2023Quote: ... Radio-labeled analytical gels were dried onto Whatman paper and exposed to Storage Phospho Screens (GE Healthcare). The phosphoscreens were scanned with the Typhoon Trio+ with an excitation wavelength of 633 nm and an emission filter of 390 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Labeled probes were then purified trough a G50 column from the Illustra MicroSpin G50 kit (GE Healthcare) and precipitated overnight with Salmon sperm DNA (Sigma) ...
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bioRxiv - Developmental Biology 2021Quote: ... Extracted proteins were immunoprecipitated with Protein A Sepharose CL-4B beads (GE Healthcare, USA) and desired primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: Protein purification was carried out with the AKTA-prime protein purification system (GE Healthcare). First ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from supernatants by protein A affinity chromatography using ÄKTA (GE Healthcare) or NGC (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The protein-antibody complexes were captured with protein-G coupled Sepharose beads (GE Healthcare) by incubation at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: ... coupled to protein G- and/or protein A-sepharose beads (GE Healthcare UK Ltd.). Proteins were eluted from beads with 0.2M glycine HCl pH2.5 ...
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bioRxiv - Immunology 2022Quote: ... purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare), and buffer-exchanged into PBS as described (12).
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bioRxiv - Bioengineering 2023Quote: ... All fusion proteins were purified by using a protein A affinity column (GE Healthcare) and analyzed on SDS-PAGE in the reducing condition.
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bioRxiv - Cancer Biology 2024Quote: ... Protein lysate was incubated with Protein G SepharoseTM 4 Fast Flow beads (GE Healthcare) for pre-clearance for 30 min at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... mTLR9-ECD was further purified by ion-exchange chromatography with a monoS 4.6/100 PE column (GE Healthcare) in 10 mM MES pH 6.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The VHH72-Fc constructs were purified from the clarified culture medium using a 5 ml MabSelect SuRe column (GE Healthcare). After a wash step with McIlvaine buffer pH 7.2 ...