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Citations for GE Life Sciences :
401 - 450 of 4395 citations for Recombinant Human EGFR protein Fc tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Recombinant CEACAM6 protein consisting of the CEACAM6 extracellular domain fused to the constant region of human IgG were produced in HEK-293 cells and purified via protein G columns (GE Healthcare, Munich, Germany) as described previously (71).
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged products were then purified using Glutathione Sepharose 4B according to manufacturer’s instruction (GE Healthcare).
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bioRxiv - Microbiology 2022Quote: ... GST-tagged PfHDAC1 was purified from the soluble fraction with glutathione Sepharose 4B beads (GE Healthcare) using 20 mM reduced glutathione ...
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bioRxiv - Cell Biology 2021Quote: ... or GFP tagged INO1 were acquired on a DeltaVision Elite imaging system (GE Healthcare Life Sciences) with a 60x/1.42 NA oil ...
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bioRxiv - Biochemistry 2021Quote: ... Binding of phage or FLAG-tagged UbVs was detected using anti-M13-HRP (GE Healthcare 27942101) or anti-FLAG-HRP antibody (Sigma-Aldrich A8592) ...
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bioRxiv - Cancer Biology 2022Quote: ... The GST-tagged constructs for the single-molecule studies were purified using glutathione Sepharose (GE Healthcare), and eluted by TEV cleavage.
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bioRxiv - Biochemistry 2022Quote: Purified GST-tagged DIDATRX and DIDATRX fragments were immobilized on Glutathione sepharose 4B beads (GE Healthcare) under saturating conditions in binding buffer (20 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... removal of the GST-tagged PreScission protease by immunoaffinity pulldown using Glutathione Sepharose 4B (GE Healthcare) was performed and the purity of GSEC complexes was assessed by SDS-PAGE and Coomassie staining (InstantBlue Protein Stain ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% fetal calf serum (FCS; GE Healthcare Bio-Sciences, Chicago, IL), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant containing recombinant was purified on TALON Superflow resin (GE healthcare) per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Cleaved GFP-8His and the His-tagged protease were removed using a HisTrap nickel column (GE Healthcare), and the untagged target AtGAAP3 was harvested from the flow-through ...
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bioRxiv - Biochemistry 2021Quote: SARM1-dN tagged with strep-tag II and flag-tag was immunoprecipitated with StrepTactin resin (GE healthcare), washed with buffer W (100 mM Tris-HCl pH8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... L7TIR and MBP tagged L7TIR were subjected to Superose 6 10/300 gel filtration column (GE Healthcare). Fractions eluted at void volume were collected ...
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bioRxiv - Biochemistry 2019Quote: ... The cleaved GST-tag and GST-tagged TEV protease was removed by Glutathione Sepharose 4B (GE Healthcare) and the remaining BMAL1 PAS-AB protein was further purified by Superdex75 gel filtration chromatography (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: ... GST tagged Mcl-1 was purified using GST-affinity chromatography (Glutathione-Sepharose resin by GE Healthcare, Fastflow). Subsequent GST-tag cleavage was carried out under rotation overnight at 4°C (Thrombin from bovine plasma ...
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bioRxiv - Cell Biology 2020Quote: ... polymerized from a 1:20 ratio of Cy5-labeled (GE Healthcare) and unlabeled tubulin ...
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bioRxiv - Microbiology 2020Quote: ... were labeled using the Cy5 monoreactive Dye pack (PA25001, GE Healthcare) and filtered using Illustra microspin G25 columns (27532501 ...
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bioRxiv - Biochemistry 2021Quote: ... were directly labeled with Cy3 following the manufacturer’s guidelines (GE Healthcare). After 45 min incubation in the dark ...
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bioRxiv - Biochemistry 2020Quote: The N-terminus of Rad6 was labeled with Cy5 (GE Healthcare). Briefly ...
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bioRxiv - Microbiology 2022Quote: LecB was labeled with Cy3 mono-reactive NHS ester (GE Healthcare) or with biotin using NHS-PEG12-biotin (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Labeled oligonucleotides were purified using microspin G-25 columns (GE Healthcare) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Labeled RNA was purified on a G-50 column (GE Healthcare), following separation in and extraction from a polyacrylamide gel as described above.
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bioRxiv - Neuroscience 2021Quote: ... Control human blood samples were processed to remove the most abundant proteins using albumin and IgG depletion SpinTrap columns (GE Healthcare Cat 28-9480-20) following the manufacturers instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... FN was depleted from FCS using gelatin sepharose-4B columns (GE Healthcare, Uppsala, Sweden), and the culture medium was supplemented with Penicillin-Streptomycin 100 U/ml and TGF-β1 (5 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... and 10% fetal calf serum (FCS; HyClone, GE Healthcare Life Sciences, Chicago, IL, USA). T cells were cultured at a density of 1×106 cells/mL at 37 °C/5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... C-terminal FLAG-tagged library of 65 chaperones was prepared from the ORFeome library (purchased from GE Healthcare), and transferred from pDONR223 into pcDNA3.1-ccdb-FLAG using gateway cloning ...
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bioRxiv - Biochemistry 2020Quote: ... The His6-tagged phytochrome samples were first purified with NiNTA affinity purification using HisTrap™ columns (GE Healthcare), followed by size-exclusion chromatography (HiLoad™ 26/600 Superdex™ 200 pg ...
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bioRxiv - Biochemistry 2019Quote: ... His6–Ub-tagged SbtB7001 was purified from cell lysate using a 5 ml HisTrap FF column (GE Healthcare): the pre-equilibrated and lysate-loaded column was washed with binding buffer (20 mM NaH2PO4 ...
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bioRxiv - Plant Biology 2021Quote: ... The 6×His-tagged AVR-PikD (ligand) was immobilised on the Series S Sensor Chip NTA (GE Healthcare) and the HMA constructs (analytes ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was collected and the His-tagged sec49K was purified with HisTrap excel Ni beads (GE Healthcare), eluted with 300 mM imidazole and dialysed against buffer containing 50% glycerol (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Hexahistidine-tagged AldA was further purified through a Superdex-200 26/60 size-exclusion column (SEC) (GE healthcare) in 25 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... All Cy5-labeled DNA was detected using Fujifilm FLA-9000 (GE Healthcare). Competition assays using specific and nonspecific oligos were performed as previously described [11] using 0.2-4 μM of competitor ...
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bioRxiv - Microbiology 2021Quote: ... labeled probes were purified using Illustra MicroSpin G-50 columns (GE Healthcare) and 40 pmol of these probes was added to the blocked membranes ...
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Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon proteasebioRxiv - Microbiology 2021Quote: ... Labeled oligonucleotides were further purified via Microspin G-25 columns (GE Healthcare) to remove unincorporated 32P-γ-ATP ...
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bioRxiv - Molecular Biology 2020Quote: ... The FAM-labeled products were imaged via Amersham Typhoon scanner (GE Healthcare) and the radioactive labeled products were visualized by phosphorimaging.
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bioRxiv - Cell Biology 2020Quote: ... Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare), anti-mouse IgG (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Bound antibodies were detected using peroxidase-labeled anti-rabbit IgG (GE Healthcare), or anti-rat IgG (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The Cy5-labeled DNA was detected with a Typhoon FLA9500 (GE Healthcare).
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bioRxiv - Biophysics 2019Quote: ... The mutant histone was purified and labeled with Cy3 maleimide (GE Healthcare) under denaturing conditions (Harada et al. ...
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bioRxiv - Genetics 2020Quote: ... [P32]-Labeled oligonucleotide probe was purified through G25 microspin column (GE Healthcare), heated at 100°C for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Radioactively labeled probes were purified using MicroSpin G-25 columns (GE Healthcare). The binding reaction mixture was prepared in a 20 μl volume ...
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bioRxiv - Genetics 2020Quote: ... [P32]-Labeled oligonucleotide probe was purified through G25 microspin column (GE Healthcare), heated at 100°C for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... the labeled RNA was purified on a G-50 column (GE Healthcare) according to manufacturer’s instructions and extracted from a polyacrylamide gel as described above following visualization on a Phosphorimager (FLA-3000 Series ...
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bioRxiv - Immunology 2023Quote: ... HRP-labeled anti-M13 phage antibody diluted 1:3000 (GE Healthcare, RPN420) was used as a detection system ...
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bioRxiv - Developmental Biology 2024Quote: ... Then labeled oligos were purified using Micro-Spin G25 columns (GE HealthCare). 3,000,000 cpm of the P32 labeled StarFire probes were used to probe the membrane.
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bioRxiv - Microbiology 2023Quote: ... Labeled oligonucleotides were further purified with Microspin G-25 columns (GE Healthcare) to remove unincorporated 32P-γ-ATP ...
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bioRxiv - Microbiology 2023Quote: ... Labeled RNAs were then purified with Miscrospin G-50 columns (GE Healthcare) and stored at -20°C for EMSA assay later.
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bioRxiv - Biochemistry 2020Quote: ... we immobilised PTEN on flow-cell (FC) 2 of a CM4 sensor chip (GE Healthcare) using amine coupling (NHS/EDC) ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... The recombinant σ38 was firstly passed through 5 mL HisTrap HP column (GE Healthcare), and the eluted protein was diluted and incubated with purified His6-TEV protein expressed by pRK793 plasmid (Kapust et al ...
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bioRxiv - Immunology 2021Quote: ... Recombinant HA molecules were purified by FPLC using a HisTrap HP Column (GE Healthcare) and eluted with 250 mM imidazole.